Effects of borate on isomerization and yeast fermentation of high xylulose solution and acid hydrolysate of hemicellulose

Hsiao, Humg-Yu; Chiang, Lin-Chang; Chen, Lifu; Tsao, George T.

doi:10.1016/0141-0229(82)90006-0

Cited by 48 publications

(15 citation statements)

References 20 publications

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“…Though the affinity of the facilitated diffusion system of C. shehatae for D-xylose is low as compared with that for glucose (Table 1), the relatively high Vmx of D-xylose transport ensures significant transport of D-xylose in the presence of glucose, at realistic concentrations of the two sugars as may be found in hemicellulose hydrolysates. For example, at concentrations of 6.02% (w/v) of D-xylose and 2.97% (w/v) of glucose, found by Hsiao et al (1982) in an acid hydrolysate of bagasse hemicellulose, the theoretical initial uptake rate of D-xylose by C. shehatae may be calculated to be 5.1 mmoles g-1 h-1 (using: Ks xylose 125 mM; Ki glucose 20 mM and Vm~x xylose 20 mmoles g-1 h-t). …”

Section: Resultsmentioning

confidence: 98%

Transport of hemicellulose monomers in the xylose-fermenting yeastCandida shehatae

Lucas

Uden

1986

Appl Microbiol Biotechnol

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Summary. Cells of Candida shehatae repressed by growth in glucose-or D-xylose-medium produced a facilitated diffusion system that transported glucose ( K s + 2 m M , Vm,x--+2.3 mmoles g-1 h-l), D-xylose (Ks + 125 mM, Vmax + 22.5 mmoles g-1 h-1) and D-mannose, but neither D-galactose nor L-arabinose.Cells derepressed by starvation formed several sugar-proton symports. One proton symport accumulated 3-0-methylglucose about 400-fold and transported glucose (Ks+0.12 mM, V m a x + 3 . 2 mmoles g-1 h-1) and D-mannose, a second proton symport transported D-xylose (Ks+ 1.0 mM, Vmax 1.4 mmoles g-1 h-1) and D-galactose, while L-arabinose apparently used a third proton symport. The stoicheiometry was one proton for each molecule of glucose or D-xylose transported. Substrates of one sugar proton symport inhibited non-competitively the transport of substrates of the other symports.Starvation, while inducing the sugar-proton symports, silenced the facilitated diffusion system with respect to glucose transport but not with respect to the transport of D-xylose, facilitated diffusion functioning simultaneously with the D-xylose-proton symport.

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Section: Resultsmentioning

confidence: 98%

Transport of hemicellulose monomers in the xylose-fermenting yeastCandida shehatae

Lucas

Uden

1986

Appl Microbiol Biotechnol

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“…Since this equation requires energy input, glycerol formation cannot solve the redox problem of the XR-XDH system. Since S. cerevisiae is capable of metabolizing xylulose, albeit at very low rates (Hsiao et al 1982;Wang and Schneider 1980), the first challenge in realizing efficient alcoholic fermentation of xylose by this yeast was the introduction of (a) heterologous enzyme(s) that convert xylose into xylulose, without causing cofactor imbalances. To quote the work of Bruinenberg et al (1983a): 'Efficient anaerobic fermentation of xylose to ethanol by yeasts apparently requires that the first two reactions of its metabolism be circumvented.…”

Section: Xylose Fermentationmentioning

confidence: 99%

Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: current status

Maris

Abbott

Bellissimi

et al. 2006

Antonie van Leeuwenhoek

437

276

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Fuel ethanol production from plant biomass hydrolysates by Saccharomyces cerevisiae is of great economic and environmental significance. This paper reviews the current status with respect to alcoholic fermentation of the main plant biomass-derived monosaccharides by this yeast. Wild-type S. cerevisiae strains readily ferment glucose, mannose and fructose via the Embden-Meyerhof pathway of glycolysis, while galactose is fermented via the Leloir pathway. Construction of yeast strains that efficiently convert other potentially fermentable substrates in plant biomass hydrolysates into ethanol is a major challenge in metabolic engineering. The most abundant of these compounds is xylose. Recent metabolic and evolutionary engineering studies on S. cerevisiae strains that express a fungal xylose isomerase have enabled the rapid and efficient anaerobic fermentation of this pentose.L-Arabinose fermentation, based on the expression of a prokaryotic pathway in S. cerevisiae, has also been established, but needs further optimization before it can be considered for industrial implementation. In addition to these already investigated strategies, possible approaches for metabolic engineering of galacturonic acid and rhamnose fermentation by S. cerevisiae are discussed. An emerging and major challenge is to achieve the rapid transition from proof-of-principle experiments under 'academic' conditions (synthetic media, single substrates or simple substrate mixtures, absence of toxic inhibitors) towards efficient conversion of complex industrial substrate mixtures that contain synergistically acting inhibitors.

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“…Interestingly, it has long been known that S. cerevisiae is able to slowly metabolise the pentose sugar d-xylulose [30,71]. This keto-isomer of xylose is phosphorylated to d-xylulose-5-phosphate by xylulokinase (XKS1, [57]) and subsequently metabolised via the non-oxidative part of the pentose phosphate pathway and glycolysis.…”

Section: Saccharomyces Cerevisiae and Fermentation Of Lignocellulosicmentioning

confidence: 99%

Development of Efficient Xylose Fermentation in Saccharomyces cerevisiae: Xylose Isomerase as a Key Component

Maris

Winkler

Kuyper

et al. 2007

Advances in Biochemical Engineering/Biotechnology

169

160

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Effects of borate on isomerization and yeast fermentation of high xylulose solution and acid hydrolysate of hemicellulose

Cited by 48 publications

References 20 publications

Transport of hemicellulose monomers in the xylose-fermenting yeastCandida shehatae

Transport of hemicellulose monomers in the xylose-fermenting yeastCandida shehatae

Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: current status

Development of Efficient Xylose Fermentation in Saccharomyces cerevisiae: Xylose Isomerase as a Key Component

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