Effects of bovine fatty acid synthase, stearoyl-coenzyme A desaturase, sterol regulatory element-binding protein 1, and growth hormone gene polymorphisms on fatty acid composition and carcass traits in Japanese Black cattle1

Matsuhashi, Tomohiro; Maruyama, Shin; Uemoto, Yoshinobu; Kobayashi, Naohiko; Mannen, Hideyuki; Abe, Tsuyoshi; Sakaguchi, Shuhei; Kobayashi, Eiji

doi:10.2527/jas.2010-3121

Cited by 126 publications

(97 citation statements)

References 45 publications

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“…This SCD gene has been extensively studied in cattle, compared with pig and chicken. The results indicated that this SCD gene has been linked to fatty acid composition in cattle (Matsuhashi et al, 2010;Ohsaki et al, 2009;Taniguchi et al, 2004). In pigs, the recent study reported the SCD gene was strongly affected in FA composition and melting point .…”

Section: Introductionmentioning

confidence: 90%

Association of FASN and SCD genes with fatty acid composition in broilers

Maharani¹,

Seo²,

Choi³

et al. 2013

Korean Journal of Agricultural Science

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: Fatty acids (FAs) were considered in activating nuclear hormone receptors that play significant roles in the cellular lipid metabolism by the regulation of several genes. Previously, fatty acid synthase (FASN) and stearoyl-CoA desaturase (SCD) genes have been known to regulating the FA metabolism. In this study, associations of FASN and SCD genes with fatty acid (FA) composition in broilers were investigated. Tissue samples from 95 Cobb 500 broilers were used for DNA extraction. The g.1222 A>G SNP located in intron 42 of FASN gene and 2 SNPs in SCD gene, one in exon 2 (g.3728A>G) and the other in exon 4 (g.12903G>A), were subjected for genotyping using PCR-RFLP method. One of the SNPs in SCD gene, SNP g.3728A>G had significant association with myristoleic acid (C14:1; P<0.05), palmitic acid (C16:0; P<0.05), palmitoleic acid (C16:1; P<0.05) and saturated FA (SFA; P<0.05). However, the SNP g.1222A>G in FASN gene had only suggestive association with arachidic acid (C20:0; P=0.08). The findings in this study suggest that the SNP in exon 2 of SCD gene can be used as a molecular marker for selecting birds having desirable FA composition in broilers.

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Section: Introductionmentioning

confidence: 90%

Association of FASN and SCD genes with fatty acid composition in broilers

Maharani¹,

Seo²,

Choi³

et al. 2013

Korean Journal of Agricultural Science

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“…When extending these investigations on commercial Japanese Black populations, both the SCD and FAS genotype have been significantly associated with 14:0, cis-9 14:1, 18:0 and cis-9 18:1 concentrations, whereas no effect of the SREBP1 genotype has been detected (Ohsaki et al, 2009;Matsuhashi et al, 2011). Elucidating the role of other genes involved in tissue lipogenesis can be expected leading to the possibility of developing DNA tests to select for muscle fatty acid composition and the identification of molecular markers for predicting the potential of individual animals to produce meat of a specific lipid composition.…”

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confidence: 99%

Recent developments in altering the fatty acid composition of ruminant-derived foods

Shingfield

Bonnet

Scollan

2013

Animal

434

443

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There is increasing evidence to indicate that nutrition is an important factor involved in the onset and development of several chronic human diseases including cancer, cardiovascular disease (CVD), type II diabetes and obesity. Clinical studies implicate excessive consumption of medium-chain saturated fatty acids (SFA) and trans-fatty acids (TFA) as risk factors for CVD, and in the aetiology of other chronic conditions. Ruminant-derived foods are significant sources of medium-chain SFA and TFA in the human diet, but also provide high-quality protein, essential micronutrients and several bioactive lipids. Altering the fatty acid composition of ruminant-derived foods offers the opportunity to align the consumption of fatty acids in human populations with public health policies without the need for substantial changes in eating habits. Replacing conserved forages with fresh grass or dietary plant oil and oilseed supplements can be used to lower medium-chain and total SFA content and increase cis-9 18:1, total conjugated linoleic acid (CLA), n-3 and n-6 polyunsaturated fatty acids (PUFA) to a variable extent in ruminant milk. However, inclusion of fish oil or marine algae in the ruminant diet results in marginal enrichment of 20-or 22-carbon PUFA in milk. Studies in growing ruminants have confirmed that the same nutritional strategies improve the balance of n-6/n-3 PUFA, and increase CLA and longchain n-3 PUFA in ruminant meat, but the potential to lower medium-chain and total SFA is limited. Attempts to alter meat and milk fatty acid composition through changes in the diet fed to ruminants are often accompanied by several-fold increases in TFA concentrations. In extreme cases, the distribution of trans 18:1 and 18:2 isomers in ruminant foods may resemble that of partially hydrogenated plant oils. Changes in milk fat or muscle lipid composition in response to diet are now known to be accompanied by tissue-specific alterations in the expression of one or more lipogenic genes. Breed influences both milk and muscle fat content, although recent studies have confirmed the occurrence of genetic variability in transcript abundance and activity of enzymes involved in lipid synthesis and identified polymorphisms for several key lipogenic genes in lactating and growing cattle. Although nutrition is the major factor influencing the fatty acid composition of ruminant-derived foods, further progress can be expected through the use of genomic or marker-assisted selection to increase the frequency of favourable genotypes and the formulation of diets to exploit this genetic potential.

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“…The SS genotype frequency in Japanese Black varied from 0.07 (9) to 0.27 (10), whereas the heterozygosity level ranged between 0.47 (9) and 0.72 (10). The remaining studied Asian cattle breeds were Brahman, Red Chittagong (5), Japanese Brown, and Mongolian (8), with S allele frequencies of 0.00, 0.05, 0.42, and 0.12, respectively.…”

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confidence: 99%

“…The 84-bp in/del polymorphism in SREBP1 was deeply investigated in Asian beef cattle breeds, especially in Japanese Black (3,(8)(9)(10) and Hanwoo, also known as Korean cattle (4,7). The SS genotype frequency in Japanese Black varied from 0.07 (9) to 0.27 (10), whereas the heterozygosity level ranged between 0.47 (9) and 0.72 (10).…”

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confidence: 99%

Insertion/deletion polymorphism of the sterol regulatory element-binding protein 1 gene in different cattle breeds

Proskura

2014

Turk J Vet Anim Sci

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Sterol regulatory element-binding protein 1 (SREBP1) is the main transcription factor linked to lipogenesis regulation. SREBP1 regulates the expression of the FASN and ACACA genes coding for the 2 major lipogenic enzymes involved in de novo fatty acid synthesis, fatty acid synthase and acetyl-CoA carboxylase, respectively (1). The SREBP1 pathway plays a key role in the regulation of milk fat synthesis, and thus the variation within the SREBP1 gene may influence fat content and especially fatty acid composition in bovine milk and meat (2). Hoashi et al. (3) determined the complete sequence of this gene. They discovered an insertion/deletion (ins/del) of 84 bp in intron 5 of the SREBP1 gene. The short (S)-and long (L)-type alleles were identified. Recently, several studies have indicated an association between this ins/ del polymorphism and fatty acid composition in the milk and meat of cattle (2,4-6). The aim of this study was to analyze the genotype distribution of the above-mentioned polymorphism in cattle populations of different breeds.The study included a total of 662 individuals of the following breeds: Montbeliard, Limousin, HolsteinFriesian, Angus, Jersey, and Charolais. DNA was extracted from whole peripheral blood, collected from the jugular vein into test tubes containing an anticoagulant (K 3 EDTA) using the MasterPure DNA Purification Kit for Blood (Epicentre Biotechnology). The primers used for amplification were those described previously (7). PCR amplifications were performed in a 10-µL volume of reaction mixture containing: approximately 60 ng of genomic DNA, 15 pmol of each primer, 1X Taq DNA polymerase buffer with (NH 4 ) 2 SO 4 , 1.5 mM MgCl 2 , 0.2 mM dNTP, 0.4 U of Taq DNA polymerase, and nucleasefree deionized water up to 10 µL. Amplifications were carried out according to the following temperature profile: initial denaturation at 94 °C for 5 min, followed by 35 cycles (denaturation at 94 °C for 30 s, primer annealing at 56 °C for 45 s, extension at 72 °C for 45 s) and final extension at 72 °C for 5 min. PCR products were separated in a 1.5% agarose gel in 1X TBE buffer and stained with ethidium bromide. After electrophoresis, DNA bands were visualized under UV light and photographed. Two alleles (L and S, corresponding to bands of 492 and 408 bp, respectively) and 3 genotypes (LL, LS, and SS) were identified.The analysis included 6 Polish cattle herds consisting of beef (Limousin, Angus, Charolais), dairy (HolsteinFriesian, Jersey), and dual-purpose (Montbeliard) breeds. Allele and genotype frequencies obtained in the present study are given in the Table. There was no polymorphism in the SREBP1 locus in dairy cattle populations. All HolsteinFriesian and Jersey individuals were LL homozygotes. Kaneda et al. (8) also reported monomorphism inAbstract: In the present study, an 84-bp insertion/deletion polymorphism of the sterol regulatory element-binding protein 1 (SREBP1) gene was investigated. The analysis included 6 Polish cattle herds consisting of beef (Limousin, Angus, Charolais), dairy...

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Effects of bovine fatty acid synthase, stearoyl-coenzyme A desaturase, sterol regulatory element-binding protein 1, and growth hormone gene polymorphisms on fatty acid composition and carcass traits in Japanese Black cattle1

Cited by 126 publications

References 45 publications

Association of FASN and SCD genes with fatty acid composition in broilers

Association of FASN and SCD genes with fatty acid composition in broilers

Recent developments in altering the fatty acid composition of ruminant-derived foods

Insertion/deletion polymorphism of the sterol regulatory element-binding protein 1 gene in different cattle breeds

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