Effects of Bovine Serum Albumin on Boar Sperm Quality During Liquid Storage at 17°C

Zhang, Xiao-Gang; Gj, Yan; Jy, Hong; Zz, Su; Gao, Yang; Qw, Li; Jh, Hu

doi:10.1111/rda.12481

Cited by 51 publications

(52 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Many studies have demonstrated that preservation of boar spermatozoa for long periods requires a decrease in their metabolic activity, which is achieved by dilution in an appropriate medium and lowering the temperature to 15-17°C (Johnson et al, 2000). In addition, acidic or hypoxic environments can reduce sperm metabolism (Zhang et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

“…The samples were transferred to the laboratory within 2 h. After evaluating the quality of the fresh semen samples, they were centrifuged at 750xg for 3 min at 17°C. The supernatant was then removed, and the semen pellet was re-suspended at a concentration of 1.0Xg 10 8 spermatozoa/mL in modified Modena solution (Zhang et al, 2015). Finally, the semen samples were all stored in an incubator at 17°C.…”

Section: Methodsmentioning

confidence: 99%

“…Of the estimated 19 million AIs of pigs that are conducted worldwide, >99% use freshly diluted liquid semen, whereas frozen semen accounts for only 1% of all pig AI because of low sperm survival rates after thawing (Gerrits et al, 2005;Frydrychová et al, 2015). Boar sperm is extremely vulnerable to low temperatures because of its low cholesterol to phospholipid ratio (Zhang et al,2015), and the quality of sperm after cryopreservation is lower than that after liquid preservation, which limits the use of frozen semen in pig AI (Yi et al, 2008). Semen in the liquid state is either used on the same day it is collected, or stored at 15-20 °C for 1-5 days (Johnson et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of negative pressure on boar semen quality during liquid storage at 17°C

Wei

et al. 2017

IJAR

View full text Add to dashboard Cite

This study aimed to investigate the influence of negative pressure on boar semen quality during liquid preservation at 17°C. Semen samples from ten large white boars were collected and pooled, divided into four equal parts, and diluted with Modena containing 0.4% (w/v) of bovine serum albumin. The semen samples were placed in a closed container with valve, and a negative pressure was applied for 2-5 minutes using a vacuum pump with a barometer. The control group had no treatment, the P200 group was treated at 200 mbar, the P400 group at 400 mbar, and the P800 group at 800 mbar. During liquid preservation, sperm motility, total antioxidant capacity, and semen H 2 O 2 content were analyzed every 24 h. The effective survival time of boar semen during preservation was evaluated. The results indicated that a suitable negative pressure decreased the effects on reactive oxygen species on boar sperm quality during liquid preservation compared with the control group. Among all the groups, the 400 mbar negative pressure group had the highest sperm motility, total antioxidant capacity, and the percentage of spermatozoa with high mitochondrial membrane potential. The P400 group also had semen H 2 O 2 content than the other groups. A suitable negative pressure improves sperm quality by reducing oxidative stress and the respiratory metabolism of sperm, and the optimum negative pressure is 400 mbar.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of negative pressure on boar semen quality during liquid storage at 17°C

Wei

et al. 2017

IJAR

View full text Add to dashboard Cite

show abstract

“…The results of SDS-PAGE of the SCP extender (unpublished data) indicated an abundance of proteins with a molecular weight of 60-66 kDa. Earlier studies demonstrated that BSA-supplemented extenders (molecular weight of approximately 65-66 kDa) reduced oxidative stress and improved the quality of boar spermatozoa stored in a liquid state at 17 o C (Zhang et al 2015). It should be noted, however, that BSA in the semen extender effused cholesterol in the plasma membrane of sperm cells, which increased the levels of reactive oxygen species (ROS) (Radomil et al 2011, Lee andPark 2015).…”

Section: Discussionmentioning

confidence: 99%

Effect of boar ejaculate fraction, extender type and time of storage on quality of spermatozoa

Dziekońska¹,

Świąder²,

Koziorowska-Gilun³

et al. 2017

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

The aim of this study was to investigate the effect the sperm-rich fraction (F1) and the post-F1 fraction (F2) on the quality of boar spermatozoa stored in a liquid state. Ejaculates were collected from three Polish Landrace boars. Each ejaculate fraction was diluted with BTS short-term extender and Safe-Cell Plus (SCP) long-term extender and stored for seven days (D1-D7) at 17 o C. Analyses included sperm motility parameters, normal apical ridge (NAR) acrosomes and plasma membrane integrity (PMI). Prior to the dilution of fractions, marked changes (p<0.05) were noted between F1 and F2 in progressive motility (PMOT), velocity average pathway (VAP) and velocity straight line (VCL). After the ejaculate was diluted, the type of fraction and type of extender significantly affected (p<0.05) PMOT, being markedly higher (p<0.05) for F1 extended in BTS. No marked changes (p<0.05) were observed between F1 and F2 extended in SCP for any of the analyzed sperm quality parameters during seven days of storage. Significantly higher (p<0.05) values of sperm quality parameters were noted in F1 compared with F2 for BTS on D7 of storage. The results of the four-way ANOVA analysis indicate that boar, fraction of ejaculate, extender type and day of storage had significant effects on the quality of boar stored spermatozoa. The F1 was characterised by higher quality of spermatozoa during storage in comparison with F2 in the short-term extender. Using the long-term extender containing the proteins allowed for a better application of F2, which could be important for the pig industry.

show abstract

“…Alvarez and Storey (1995) reported that BSA is a very potent inhibitor of lipid peroxidation in the spermatozoa, and this type of damage reduces sperm motility. It was proven that BSA added to the Modena extender exhibits antioxidant properties, reduces oxidative stress, and enhances the quality of boar semen stored at a temperature of 17 o C (Zhang et al 2015). The content of antioxidants in the stored semen plays an important role in the survivability of the cryopreserved spermatozoa (Bilodeau et al 2000, Funahashi andSano 2005).…”

Section: Discussionmentioning

confidence: 99%

Effects of storage in different semen extenders on the pre-freezing and post-thawing quality of boar spermatozoa

Dziekońska¹,

Zasiadczyk²,

Lecewicz³

et al. 2015

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

The aim of this study was to investigate the effects of storage of semen in different commercial extenders on the pre-freezing and post-thawing quality of boar spermatozoa. Semen was diluted in BTS, Androhep (AH) and Gedil (GD), stored for 24 h at 17 o C, and then frozen in accordance with the cryopreservation protocol. Analyses of the quality of spermatozoa included: motility, normal apical ridge (NAR) acrosome, plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), measurements of ATP content and activity of superoxidase dismutase (SOD) and glutathione peroxidase (GPx). Prior to the freezing process, no significant effect of the extender on the sperm quality parameters was noted. After thawing the spermatozoa it was demonstrated that the type of extender used influenced PMI, MMP, ATP content and activity of GPx. In the AH extender the percentage of spermatozoa with PMI and ATP content in spermatozoa was significantly higher (P<0.05) as compared to the BTS or GD extenders. In addition, semen stored in the AH was characterised by a statistically higher (P<0.05) percentage of spermatozoa with MMP and increased activity of GPx as compared with the BTS. The results obtained indicate that for the cryopreservation process, boar spermatozoa stored for 24 hours in liquid state can be used. However, the type of extender used prior to freezing may have a significant effect on the post-thawing quality of the spermatozoa. The AH extender better secured the quality of thawed boar spermatozoa as compared with the BTS or GD.

show abstract

Effects of Bovine Serum Albumin on Boar Sperm Quality During Liquid Storage at 17°C

Cited by 51 publications

References 34 publications

Effects of negative pressure on boar semen quality during liquid storage at 17°C

Effects of negative pressure on boar semen quality during liquid storage at 17°C

Effect of boar ejaculate fraction, extender type and time of storage on quality of spermatozoa

Effects of storage in different semen extenders on the pre-freezing and post-thawing quality of boar spermatozoa

Contact Info

Product

Resources

About