Effects of brassinosteroid on cotton regeneration via somatic embryogenesis

Abstract

Brassinolide (BR), which is the most biologically active brassinosteroid, was used to examine the potential effect of hormone on cotton somatic embryogenesis. Ten-day-old cotton (Gossypium hirsutum L., cv. Cooker) seedlings were used for explant source and hypocotyls were removed and cultured on MS basal medium with B5 vitamins supplemented with 1 mg/L 6-benzylaminopurine + 0.5 mg/L kinetin for callus induction. After one month proliferating calli pieces were collected and cultured on MS basal medium containing various concentrations of BR (0.1, 0.5, 1.0 µM) with their controls. BR treatments were negatively effective on the fresh weight of calli when compared to control. Differential somatic embryogenesis maturation rates due to BR treatment were observed. Somatic embryogenesis was stimulated especially for transition to cotyledonary phase at 0.5 mg/L BR. Histological preparations from embryogenic calli and somatic embryos at different stages of development revealed the spontaneous polyploidisation during early somatic embryogenesis on BRtreated calli. Present results suggest that BR negatively effected calli growth, however, had a stimulating role in maturation of somatic embryos.

Effects of homobrassinolide in barley callus culture

“…BL along with IAA and BA increased shoot regeneration and shoot height in Spartina patens (Lu et al 2003). BR stimulated somatic embryogenesis in cotton (Aydin et al 2006). BRs increased protein content and CAT and SOD activity in Brassica juncea L. under field conditions (Sirhindi et al 2009).…”

“…BL along with indole-3-acetic acid (IAA) and 6-benzylaminopurine (BA) increased callus fresh and dry weight in Spartina patens, a halophyte monocot (Lu et al 2003). BR treatment was also negatively effective on the fresh weight of calli in cotton (Aydin et al 2006). BL along with IAA and BA increased shoot regeneration and shoot height in Spartina patens (Lu et al 2003).…”

Plant Wnt:Deciphering a Novel Signalling Pathway in Plants

“…On the contrary, based on the signalling pattern, Br signalling in plants has been correlated with metazoan Wnt like signalling. It is reported that Br is involved in the process of callusing, somatic embryogenesis 82 and promotes fibre initiation and elongation in Gossypium 83,84 . This role of Br is rather comparable to that of Wnt's action in animals where Wnt promotes embryogenesis and cell reprogramming.…”

Bamboo Regeneration via Embryogenesis and Organogenesis

“…Different kinds of plant growth regulators are needed in different stage of bamboo regeneration, and auxin and cytokinin are the critical components for the morphogenesis in vitro. Besides, ethylene, abscisic acid (ABA) and brassinosteroid (BR) will also have different effects on embryogenesis and organogenesis (Aydin et al, 2006;Torrizo & Zapata, 1986;Vain et al, 1989).…”