Effects of Brazilian and Bulgarian propolis on bactericidal activity of macrophages against Salmonella Typhimurium

Orsi, R. O.; Sforcin, José Maurício; Funari, Sílvia Regina Cunha; Bankova, Vassya

doi:10.1016/j.intimp.2004.10.003

Cited by 89 publications

(64 citation statements)

References 28 publications

(32 reference statements)

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“…A própolis tem sido utilizada no combate a diversas patologias, sendo amplamente utilizada como antibacteriana, antiviral, antitumoral, antifúngica, antioxidante e imunomoduladora, apresentando muitas outras atividades biológicas (Dobrowolski et al, 1991;Amoros et al, 1992;Castro, 2001;Fernandes Jr. et al, 2005;Gonsales et al, 2005a;Orsi et al, 2005;Orsi et al, 2006).…”

Section: Introductionunclassified

Propriedade físico-química da própolis em função da sazonalidade e método de produção

et al. 2010

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PALAVRAS CHAVE ADICIONAISApis mellifera. Abelhas africanizadas. ADDITIONAL KEYWORDSApis mellifera. Africanized bees. RESUMOO objetivo do presente trabalho foi avaliar o efeito da sazonalidade sobre algumas caracterís-ticas físico-químicas (extrato seco, flavonóides totais, pH e atividade de oxidação) dos extratos alcoólicos (EAP) da própolis obtida mensalmente, durante um ano, por diferentes técnicas (coletor de própolis inteligente, tela plástica e raspagem) em quinze colméias de abelhas Apis mellifera africanizadas. Não foram observadas diferenças significativas (p>0,05) nas análises físico-quími-cas dos EAP, em função da sazonalidade e téc-nica de produção. SUMMARYThe aim of this work was to investigate monthly, for one year the seasonality effect on the physicochemical properties (dry weight, flavonoids contents, pH and antioxidant activity) of ethanolic extract (EAP) of propolis produced by three different techniques (intelligent propolis collector, plastic screen or scraping) fifteen colonies of africanized bees. No differences (p>0.05) in the physicochemical properties due to the technique of propolis production and seasonality were observed. INTRODUÇÃOA própolis tem sido utilizada no combate a diversas patologias, sendo amplamente utilizada como antibacteriana, antiviral, antitumoral, antifúngica, antioxidante e imunomoduladora, apresentando muitas outras atividades biológicas (Dobrowolski et al., 1991;Amoros et al., 1992;Castro, 2001;Fernandes Jr. et al., 2005;Gonsales et al., 2005a;Orsi et al., 2005;Orsi et al., 2006).Entretanto, diversos fatores podem interferir na qualidade da própolis, como sazonalidade e tipo de coletor utilizado (Lima, 2006). No Brasil, para assegurar a qualidade do produto, utilizam-se as normas do Considerando-se que a procura pela própolis tem crescido nos últimos anos, principalmente pelo interesse da população para os chamados produtos naturais, o levantamento de dados que contribuam para o conhecimento de fatores que possam interferir com a qualidade do produto, são de suma importância.Diante do exposto, o objetivo do presente trabalho foi avaliar o efeito da sazonalidade sobre algumas características físico-químicas (extrato seco, flavonóides totais, pH e atividade de oxidação) dos extratos alcoólicos (EAP) da própolis obtida por diferentes técnicas de produção (coletor

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Section: Introductionunclassified

Propriedade físico-química da própolis em função da sazonalidade e método de produção

et al. 2010

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“…General medicinal uses of propolis include treatment of the cardiovascular and blood systems disorder (anemia), respiratory apparatus (for various infections), dental care (Ikeno et al 1991), dermatology (tissue regeneration, ulcers, excema, wound healing--particularly burn wounds, mycosis, mucous membrane infections and lesions), cancer treatment (Grunberger et al 1988;Scheller et al 1989b;Hausen et al 1992), immune system support and improvement (Scheller et al 1989a), digestive tract disorders (ulcers and infections), liver protection and support and many others. Propolis compounds including flavonoids, phenolic acids and its esters have anti-inflammatory, antibacterial, antiviral, immunomodulatory, antioxidant and antiproliferative effects (Hu et al 2005;Noelker et al 2005;Orsi et al 2005;Kim et al 2006). Propolis is shown to inhibit cell division of unmoral cell and protein synthesis.…”

Section: Introductionmentioning

confidence: 99%

Effect of the water extracts of propolis on stimulation and inhibition of different cells

et al. 2007

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The water extracts of propolis (WEP) could inhibit growth of different cell lines namely McCoy, HeLa, SP2/0, HEp-2, and BHK21 and stimulate growth of normal cell named human lymphocyte, rat kidney, rat liver, and rat spleen. In these experiments 1 and 2 mg of WEP were added to 1 ml RPMI media with 5% FCS. Cell counts and cell viability of propolis-treated and propolis-free cells were assessed by Trypan blue dye exclusion test and MTT assay. The results showed that in case of McCoy, HeLa, SP20, HEp-2, and BHK21 cell lines, the water extracts of propolis could inhibit cell growth as well as reduction on size of the cells. In contrast the same amount of WEP could stimulate growth of normal cells up to 60% with the same concentration used for cell lines. Thus our study indicates that although WEP consists only of the soluble part of propolis, it enables to inhibit different cell lines and increase growth of normal cells. This indicates also that WEP contains the specific compounds with bioactivity against cell lines. Although propolis contain different number of compounds it is clear that WEP has enough biological compounds useful for the treatment of some diseases, medical and related applications.

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“…The wide use of propolis is due to its biological properties, such as antimicrobial, immunomodulatory, antitumoral activity [14][15][16][17][18][19][20][21] . Moreover, some clinical and experimental investigations have pointed to the antiparasite properties of propolis extracts 22,23 .…”

Section: Resultsmentioning

confidence: 99%

The scolocidal effect of propolis on protoscoleces and daugther cysts

Özçelık¹,

Sümer²,

Değerlı³

2015

Cumhuriyet Med J

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SUMMARYObjective: Scolocidal elements are used in the aim of not scattering the protoscoleces' outside the cyst during the treatment of cyst hydatid in our present-day. It is hoped from the scolocidal materials; besides its effect on protoscoleces, not being toxic, being easy practicable, not causing sclerozan cholanjit and if possible, penetrating in the daughter vesicules. In this research, the effect of propolis which is a bee product on protoscoleces were investigated. Methods: In our work, daughter vesicules and protoscoleces obtain from the liver of a patient during surgery and propolis from the Black Sea Region were used. Viability tests were done by using 0.1% eosine solution. After propolis disolved in dimethylsulfoxide, within the range of 50 mg/mL and 0.010 mg/mL using by 0.9% serum physiological solution 100 µl into each of the tube and in 1.,3.,5.,10.,20.,and 30. minutes the rates of viability of protoscolex (4000 protoscolex/mL) are fixed. The research were subjected to daughter vesicules that is 4-7 mm in diameters. The concentration of propolis at 0.80 and 3.12 mg/mL were investigated viability rates of protoscolex at different incubation time and whether the possibilty of penetration to the daughter vesicules. Results: It was seen that protoscolexs were effected by the Propolis at 0.100 mg/mL concentration in the first minute, at 0.050 mg/mL concentration in the 3. minutes, at 0.025 mg/mL concentration at 10. minutes and at 0.010 mg/mL concentration in 20. minutes. For protoscolex in the vesicules at 0.80 mg/mL concentration in 20. minute, in 3.12 mg/mL concentration in 10. minutes were found to be lethal. Conclusions: As the protoscolex could be killed by Propolis which penetrate to daughter cysts in a very short time as 10 minutes, we thing that it will be beneficial completing advanced studies.

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Effects of Brazilian and Bulgarian propolis on bactericidal activity of macrophages against Salmonella Typhimurium

Cited by 89 publications

References 28 publications

Propriedade físico-química da própolis em função da sazonalidade e método de produção

Propriedade físico-química da própolis em função da sazonalidade e método de produção

Effect of the water extracts of propolis on stimulation and inhibition of different cells

The scolocidal effect of propolis on protoscoleces and daugther cysts

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