Effects of butanol on high value product production in Schizochytrium limacinum B4D1

Zhang, Ke; Chen, Limei; Liu, Jianmin; Gao, Feng; He, Rong; Chen, Wuxi; Guo, Wei; Chen, Shulin; Li, Demao

doi:10.1016/j.enzmictec.2017.03.007

Cited by 42 publications

(31 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In conclusion, most key genes related to the synthesis of fatty acid and fatty acid precursors were significantly downregulated under the H condition and significantly decreased fatty acid bioaccumulation in S. limacinum B4D1, in agreement with the experimental results. Previous studies have reported that high concentrations of other short-chain alcohols, ethanol (40 ml/liter) and butanol (8 g/liter), lead to a decrease in both biomass (ϳ70% and ϳ60%, respectively) and lipid accumulation (ϳ50% and ϳ40%, respectively) (23,40). In addition, ethanol and butanol also altered the fatty acid profiles of Schizochytrium.…”

Section: Discussionmentioning

confidence: 92%

“…There are two known metabolic pathways for fatty acid biosynthesis in Schizochytrium: a fatty acid synthase pathway and a polyunsaturated fatty acid synthase pathway (23,25,26). Acetyl-CoA and NADPH are considered the same precursors for both pathways (11).…”

Section: Discussionmentioning

confidence: 99%

“…The medium was divided into seed medium and fermentation medium. The seed medium and conditions were the same as those used in our previous study (23). The fermentation medium was composed of the following (per liter): artificial seawater salt, 15 g; glucose, 80 g; yeast extract, 4 g; peptone, 4 g; MgCl 2 •6H 2 O, 6.4 g; CaCl 2 •2H 2 O, 1 g; MgSO 4 •7H 2 O, 5 g; and KCl, 2 g. Both media were sterilized at 115°C for 20 min before inoculation.…”

Section: Methodsmentioning

confidence: 99%

“…(iii) Measurement of dry cell weight. The cells were harvested by centrifugation (14,972 ϫ g, 5 min), washed with distilled water, and then dried in a freeze dryer (FD-1C-50; BJBYK Co., Beijing, China) until the weight was constant (23).…”

Section: Methodsmentioning

confidence: 99%

“…However, little is known regarding the pathway of Schizochytrium's astaxanthin biosynthesis. In a previous study, Zhang et al have proposed potential pathways for the production of fatty acids, squalene, sterol, and carotenoids in Schizochytrium and have also observed an interesting phenomenon in which the red color of Schizochytrium darkens with the induction of butanol (23), owing to astaxanthin accumulation.…”

mentioning

confidence: 98%

See 4 more Smart Citations

Effects of Methanol on Carotenoids as Well as Biomass and Fatty Acid Biosynthesis in Schizochytrium limacinum B4D1

Liao

Gao

et al. 2019

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

Schizochytrium is a promising source for the production of docosahexaenoic acid and astaxanthin. The effects of different methanol concentrations on astaxanthin, biomass, and production of the lipids, squalene, and total sterol in Schizochytrium limacinum B4D1 were investigated. Astaxanthin began to accumulate when the methanol concentration reached 3.2% and peaked at 5.6% methanol, with a 2,000-fold increase over that in the control. However, under cultivation with 5.6% methanol, the biomass, lipids, squalene, and total sterol decreased to various degrees. Transcriptomic analysis was performed to explore the effects of different methanol concentrations (0%, 3.2%, and 5.6%) on the expression profile of B4D1. Three key signaling pathways were found to play important roles in regulating cell growth and metabolism under cultivation with methanol. Five central carbon metabolism-associated genes were significantly downregulated in response to 5.6% methanol and thus were expected to result in less ATP and NADPH being available for cell growth and synthesis. High methanol conditions significantly downregulated three genes involved in fatty acid and squalene/sterol precursor biosynthesis but significantly upregulated geranylgeranyl diphosphate synthase, lycopene β-cyclase, and β-carotene 3-hydroxylase, which are involved in astaxanthin synthesis, thus resulting in an increase in the levels of precursors and the final production of astaxanthin. Additionally, the transcriptional levels of three stress response genes were upregulated. This study investigates gene expression profiles in the astaxanthin producer Schizochytrium when grown under various methanol concentrations. These results broaden current knowledge regarding genetic expression and provide important information for promoting astaxanthin biosynthesis in Schizochytrium. IMPORTANCE Schizochytrium strains are usually studied as oil-producing strains, but they can also synthesize other secondary metabolites, such as astaxanthin. In this study, methanol was used as an inducer, and we explored its effects on the production of astaxanthin, a highly valuable substance in Schizochytrium. Methanol induced Schizochytrium to synthesize large amounts of astaxanthin. Transcriptomic analysis was used to investigate the regulation of signaling and metabolic pathways (mainly relative gene expression) in Schizochytrium grown in the presence of various concentrations of methanol. These results contribute to the understanding of the underlying molecular mechanisms and may aid in the future optimization of Schizochytrium for astaxanthin biosynthesis.

show abstract

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 98%

See 3 more Smart Citations

Effects of Methanol on Carotenoids as Well as Biomass and Fatty Acid Biosynthesis in Schizochytrium limacinum B4D1

Liao

Gao

et al. 2019

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

Selecting Endogenous Promoters for Improving Biosynthesis of Squalene in Schizochytrium sp.

Nong,

Zhang,

et al. 2024

Biotechnology Journal

View full text Add to dashboard Cite

Squalene (C30H50) is an acyclic triterpenoid compound renowned for its myriad physiological functions, such as anticancer and antioxidative properties, rendering it invaluable in both the food and pharmaceutical sectors. Due to the natural resource constraints, microbial fermentation has emerged as a prominent trend. Schizochytrium sp., known to harbor the intact mevalonate acid (MVA) pathway, possesses the inherent capability to biosynthesize squalene. However, there is a dearth of reported key genes in both the MVA and the squalene synthesis pathways, along with the associated promoter elements for their modification. This study commenced by cloning and characterizing 13 endogenous promoters derived from transcriptome sequencing data. Subsequently, five promoters exhibiting varying expression intensities were chosen from the aforementioned pool to facilitate the overexpression of the squalene synthase gene squalene synthetase (SQS), pivotal in the MVA pathway. Ultimately, a transformed strain designated as SQS‐3626, exhibiting squalene production 2.8 times greater than that of the wild‐type strain, was identified. Finally, the optimization of nitrogen source concentrations and trace element contents in the fermentation medium was conducted. Following 120 h of fed‐batch fermentation, the accumulated final squalene yield in the transformed strain SQS‐3626 reached 2.2 g/L.

show abstract

Microbial Biofactories: A Promising Approach Towards Sustainable Omega-3 Fatty Acid Production

Rawat,

Mundepi,

Pant

et al. 2023

Food Microbial Sustainability

View full text Add to dashboard Cite

Effects of butanol on high value product production in Schizochytrium limacinum B4D1

Cited by 42 publications

References 27 publications

Effects of Methanol on Carotenoids as Well as Biomass and Fatty Acid Biosynthesis in Schizochytrium limacinum B4D1

Effects of Methanol on Carotenoids as Well as Biomass and Fatty Acid Biosynthesis in Schizochytrium limacinum B4D1

Selecting Endogenous Promoters for Improving Biosynthesis of Squalene in Schizochytrium sp.

Microbial Biofactories: A Promising Approach Towards Sustainable Omega-3 Fatty Acid Production

Contact Info

Product

Resources

About