Effects of butyrate and insulin and their interaction on the DNA synthesis of rumen epithelial cells in culture

Neogrády, S.; Gálfi, P.; Kutas, F.

doi:10.1007/bf01990459

Cited by 33 publications

(13 citation statements)

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“…The reduction of apoptosis is another important factor that can increase cell accumulation in rumen mucosa (32,36,45). For example, by downregulation of IGFBP3, butyrate is thought to slow apoptosis, thereby encouraging rumen tissue growth (32,45).…”

Section: Discussionmentioning

confidence: 99%

Modulation of vH⁺-ATPase is part of the functional adaptation of sheep rumen epithelium to high-energy diet

Kuzinski

Žitňan

Albrecht

et al. 2012

American Journal of Physiology-Regulatory, Integrative and Comp

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Ruminal vacuolar H(+)-ATPase (vH(+)-ATPase) activity is regulated by metabolic signals. Thus, we tested whether its localization, expression, and activity were changed by different feeding. Young male sheep (n = 12) were either fed hay ad libitum (h) or hay ad libitum plus additional concentrate (h/c) for 2 wk. The vH(+)-ATPase B subunit signal was predominantly found in the cell membrane and cytosol of rumen epithelial cells (REC) with basal/parabasal phenotype. The elevated number (threefold) of these cells in rumen mucosa of h/c-fed sheep reflects a high proliferative capacity and, explains the 2.3-fold increase of the total number of vH(+)-ATPase-expressing REC. However, in accordance with a 58% reduction of the vH(+)-ATPase B subunit mRNA expression in h/c-fed sheep, its protein amount per single REC was decreased. Using the fluorescent probe BCECF and selective inhibitors (foliomycin, amiloride), the contribution of vH(+)-ATPase and Na(+)/H(+) exchanger to intracellular pH (pH(i)) regulation was investigated. REC isolated from h/c-fed sheep keep their pH(i) at a significantly higher level (6.91 ± 0.03 vs. 6.74 ± 0.05 in h-fed sheep). Foliomycin or amiloride decreased pH(i) by 0.16 ± 0.02 and 0.57 ± 0.04 pH units when applied to REC from h-fed sheep, but the effects were markedly reduced (-88 and -33%) after concentrate feeding. Nevertheless, we found that REC proliferation rate and [cAMP](i) were reduced after foliomycin-induced vH(+)-ATPase inhibition. Our results provide the first evidence for a role of vH(+)-ATPase in regulation of REC proliferation, most probably by linking metabolically induced pH(i) changes to signaling pathways regulating this process.

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Section: Discussionmentioning

confidence: 99%

Modulation of vH⁺-ATPase is part of the functional adaptation of sheep rumen epithelium to high-energy diet

Kuzinski

Žitňan

Albrecht

et al. 2012

American Journal of Physiology-Regulatory, Integrative and Comp

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“…Feeding induced adaptations of rumen epithelium growth are largely due to propionate and butyrate in the rumen fluid [34,35,41,68,85,101]. In the 1980's, German and Israeli scientists [27,71,78] showed that a reduction takes place in the surface area of the rumen papillae due to reduced feed intake in the dry period, and that this reduction could partly be prevented by giving an energy-rich lactation feed throughout the dry period.…”

Section: Rumen Epitheliummentioning

confidence: 99%

Priming the dairy cow for lactation: a review of dry cow feeding strategies

Friggens¹,

Andersen²,

Larsen³

et al. 2004

Anim. Res.

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-This paper reviews important aspects of dry cow feeding with emphasis placed on the preparation of the cow for the subsequent lactation. A number of feeding strategies, which have been proposed in recent years, are examined, alongside considerations of the effects of dry period length, levels of body reserves at calving and parity. A number of studies have reported the effect of varying the length of the dry period (in the range 0 to 70 days) on subsequent production and health. An 8 week dry period seems optimal though this should be adjusted to allow for the body condition score of cows at drying off. Avoiding extremes of body condition score at calving is desirable, so condition should be monitored throughout the lactation cycle. Attempting to achieve large changes in body reserves in the dry period has been associated with increased problems postpartum. The feeding strategies that are reviewed are strategies to decrease body mobilisation, prime for body mobilisation, prime the rumen for increased intake, prime for milk protein production and prime for calcium mobilisation and absorption. These strategies are also evaluated relative to their biological basis and reported physiological effects. Feeding high starch feeds in the latter part of the dry period was found not to provide clear benefits in terms of decreasing early lactation body mobilisation or increasing early lactation intake. Strategies aimed at priming the cow for the increased mobilisation of body lipid and body calcium that occurs in early lactation appear more favourable both from the point of view of making biological sense and from the available results reviewed. dry period / feeding / body condition score / dairy cattle / health Résumé -Préparation des vaches laitières à la lactation : bilan des stratégies d'alimentation des vaches taries. Cet article passe en revue les aspects importants de l'alimentation des vaches taries en mettant l'accent sur la préparation de la vache à la lactation suivante. Plusieurs stratégies d'alimentation proposées au cours des dernières années sont examinées, dans le même temps que des considérations portant sur les effets de la durée de la période sèche, du niveau des réserves corporelles au vêlage et du rang de lactation. Un certain nombre d'études ont montré que le fait de * Corresponding author: n.friggens@agrsci.dk 454 N.C. Friggens et al.faire varier la durée de la période sèche (dans une plage de 0 à 70 jours) avait un effet sur la production et la santé lors de la lactation suivante. Une période sèche de 8 semaines semble optimale, quoiqu'elle doive être ajustée en tenant compte de la note d'état corporel des vaches au moment du tarissement. Il est préférable d'éviter des notes extrêmes au vêlage ; l'état corporel devrait donc être surveillé tout au long du cycle de lactation. Les tentatives de vouloir effectuer de grandes modifications du niveau des réserves corporelles pendant la période sèche ont été associées à une augmentation des problèmes postpartum. Les stratégies d'alimentation exposé...

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“…Daily fluctuations in butyrate concentration have a very pronounced positive effect on the proliferation of the ruminal epithelium in 6itro (Sakata & Tamate, 1978). The positive effect of propionate and butyrate on proliferation could be explained by the metabolization of the VFA in the ruminal mucosa (Sander et al, 1959), the indirect effect of VFA on blood flow in the mucosa (Fell & Weekes, 1975) or the indirect effect of butyrate and propionate on insulin secretion (Brockman, 1984), which has a positive effect on cell proliferation in the rumen epithelium in sheep (Sakata & Tamate, 1978, 1979Neogrady et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

Effect of Dry Cow Feeding Strategy on Rumen pH, Concentration of Volatile Fatty Acids and Rumen Epithelium Development

Andersen¹,

Sehested²,

Ingvartsen³

1999

Acta Agriculturae Scandinavica, Section A - Animal Science

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Effects of butyrate and insulin and their interaction on the DNA synthesis of rumen epithelial cells in culture

Cited by 33 publications

References 12 publications

Modulation of vH⁺-ATPase is part of the functional adaptation of sheep rumen epithelium to high-energy diet

Modulation of vH⁺-ATPase is part of the functional adaptation of sheep rumen epithelium to high-energy diet

Priming the dairy cow for lactation: a review of dry cow feeding strategies

Effect of Dry Cow Feeding Strategy on Rumen pH, Concentration of Volatile Fatty Acids and Rumen Epithelium Development

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Effects of butyrate and insulin and their interaction on the DNA synthesis of rumen epithelial cells in culture

Cited by 33 publications

References 12 publications

Modulation of vH+-ATPase is part of the functional adaptation of sheep rumen epithelium to high-energy diet

Modulation of vH+-ATPase is part of the functional adaptation of sheep rumen epithelium to high-energy diet

Priming the dairy cow for lactation: a review of dry cow feeding strategies

Effect of Dry Cow Feeding Strategy on Rumen pH, Concentration of Volatile Fatty Acids and Rumen Epithelium Development

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Modulation of vH⁺-ATPase is part of the functional adaptation of sheep rumen epithelium to high-energy diet

Modulation of vH⁺-ATPase is part of the functional adaptation of sheep rumen epithelium to high-energy diet