Effects of CaCl2 hydrothermal treatment of titanium implant surfaces on early epithelial sealing

Oshiro, Wakana; Ayukawa, Yasunori; Atsuta, Ikiru; Furuhashi, Akihiro; Yamazoe, Jyunichi; Kondo, Ryosuke; Sakaguchi, Mami; Matsuura, Yuri; Tsukiyama, Yoshihiro; Koyano, Kiyoshi

doi:10.1016/j.colsurfb.2015.04.025

Cited by 30 publications

(45 citation statements)

References 25 publications

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“…These implants were remodeled from previously described designs . The surface topographies were similar to the experimental plates used in our culture experiments …”

Section: Methodsmentioning

confidence: 99%

“…4,9 The surface topographies were similar to the experimental plates used in our culture experiments. 10 Immunohistochemistry (light microscopy) At 4 weeks after implantation, the rats were euthanized. The oral mucosa was removed from the maxillary bone, and cut the sections on coronal plane using a cryostat (−20 C).…”

Section: Animalsmentioning

confidence: 99%

“…Adhesion assays of OECs were separated adherent and nonadherent cells to by using a rotary shaker (NX-20; Nissin, Tokyo, Japan). 10 Percentage of adherent cells was defined as the adhesive power of the cells.…”

Section: Adhesion Assaymentioning

confidence: 99%

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Epithelial sealing effectiveness against titanium or zirconia implants surface

Atsuta

Ayukawa

Furuhashi

et al. 2019

J Biomedical Materials Res

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The aims of implant treatment now involve not only restoration of mastication function, but also recovery of esthetics. Currently, zirconia is widely used as an esthetic material for implant abutment. Therefore, it is very important to understand the efficacy of zirconia for epithelial sealing as an implant material. We compared the effects of materials on the sealing of the peri‐implant epithelium (PIE) to titanium (Ti) or zirconia (Zr) implants, for application to clinical work. Maxillary first molars were extracted from rats and replaced with Ti or Zr implants. The sealing of the PIE to the implants was evaluated with immunohistochemistry observation and HRP analysis. The morphological and functional changes in rat oral epithelial cells (OECs) cultured on Ti or Zr plates were also evaluated. After 4 weeks, the PIE on the Ti and Zr implants showed similar structures. The Zr implants appeared to form a weak epithelial seal at the tissue–implant interface, and exhibited markedly less adhesive structures than the Ti implants under electron microscopic observation. In the in vitro experiments, decreased expression levels of adhesion proteins were observed in OECs cultured on Zr plates compared with those cultured on Ti plates. In addition, the cell adherence on Zr plates was reduced, while the cell migration was low on Ti plates. Zr is a better choice for an esthetic implant material, but needs further improvement for integration with the epithelial wound healing process around a dental implant. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2019.

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“…These implants were remodeled from previously described designs . The surface topographies were similar to the experimental plates used in our culture experiments …”

Section: Methodsmentioning

confidence: 99%

Section: Animalsmentioning

confidence: 99%

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Epithelial sealing effectiveness against titanium or zirconia implants surface

Atsuta

Ayukawa

Furuhashi

et al. 2019

J Biomedical Materials Res

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“…All experimental procedures were approved by the Ethics Committee on Animal Experimentation at Kyushu University (Approval Number: A25-133-0), Japan, in accordance with the ARRIVE guidelines and the Guidelines of the Japanese Physiological Society. Surgical implantation was performed in accordance with previously published protocols [ 4 , 16 ]. Briefly, 6-week-old Wistar rats (27 males, 120–150 g) underwent immediate implant placement as follows: the maxillary right first molars were removed and an experimental implant was screwed into the socket under systemic chloral hydrate under systemic anesthesia.…”

Section: Methodsmentioning

confidence: 99%

The influence of systemically or locally administered mesenchymal stem cells on tissue repair in a rat oral implantation model

et al. 2018

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BackgroundMultipotent mesenchymal stem cells (MSCs) are used clinically in regenerative medicine. Our previous report showed systemically injected MSCs improved peri-implant sealing and accelerated tissue healing. However, the risks of systemic MSC administration, including lung embolism, must be considered; therefore, their local application must be assessed for clinical safety and efficacy. We investigated differences in treatment effect between local and systemic MSC application using a rat oral implantation model.MethodsRat bone marrow-derived MSCs were isolated and culture-expanded. The rat’s right maxillary first molars were extracted and replaced with experimental titanium implants. After 24 h, MSCs (1 × 106/ml) were systemically or locally injected into recipient rats via the tail vein (systemic group) or buccal subcutaneous tissue (local group), respectively. Rats treated in the absence of MSCs were included as a control (control group). The maxillary epithelium was assessed histologically after 4 weeks to evaluate laminin-332 (Ln-332) distribution and horseradish peroxidase invasion, as indicators of peri-implant epithelium (PIE) formation and PIE sealing to the implant surface, respectively. The effect of MSCs on rat oral epithelial cell (OEC) morphology was determined by coculture.ResultsSystemic group MSCs accumulated early at the peri-implant mucosa, while local group MSCs were observed in various organs prior to later accumulation around the implant surface. PIE formation and Ln-332-positive staining at the implant interface were enhanced in the systemic group compared with the local and control groups. Furthermore, OEC adherence on implants was reduced in high-density compared with low-density MSC cocultures.ConclusionsLocal MSC injection was more ineffective than systemic MSC injection at enhancing PIE sealing around titanium implants. Thus, although local MSC administration has a wide range of applications, further investigations are needed to understand the exact cellular and molecular mechanisms of this approach prior to clinical use.

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“…Unfortunately, this means that there must be a trade-off between the ability to deposit apatite particles and the esthetic appearance of titanium implants. Thus far, there are many reports concerning the effects of hydrothermal treatment on the surface of metallic titanium and its physico-chemical and biological properties [31][32][33][34][35][36][37]. Fluorine-contaminated titanium implants were strongly discolored after autoclaving (by applying high-pressure steam sterilization treatment at 132°C for 20 min) due to the growth of a surface oxide [38].…”

Section: Introductionmentioning

confidence: 99%