Effects of CaCl2 Treatment Alleviates Chilling Injury of Loquat Fruit (Eribotrya japonica) by Modulating ROS Homeostasis

Abstract

The effects of calcium chloride (CaCl2) treatment on chilling injury (CI), reactive oxygen species (ROS) metabolism, and ascorbate-glutathione (AsA-GSH) cycle in loquat fruit at 1 °C storage for 35 d were investigated. The results indicated that CaCl2 treatment remarkably suppressed the increase in browning index and firmness as well as the decrease in extractable juice rate. CaCl2 treatment also decreased the production of superoxide radical (O2•−), hydrogen peroxide (H2O2) content, but increased the 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical (OH•) scavenging ability, the activities of superoxide dismutase (SOD), catalase (CAT), and their gene expressions. Moreover, compared to the control loquat fruit, CaCl2-treated fruit maintained higher contents of AsA, GSH, higher levels of activities of ascorbate peroxidase (APX), glutathione reductase (GR), dehydroascorbate reductase (DHAR), and monodehydroascorbate reductase (MDHAR) and expressions of EjAPX, EjGR, EjMDHAR, and EjDHAR, but exhibited lower glutathione disulfide (GSSG) content. These results suggested that CaCl2 treatment alleviated CI in loquat fruit through enhancing antioxidant enzymes activities and AsA-GSH cycle system to quench ROS.

Postharvest Treatment of ‘Florida Prince’ Peaches with a Calcium Nanoparticle–Ascorbic Acid Mixture during Cold Storage and Its Effect on Antioxidant Enzyme Activities

“…Hence, AA can be used as a stabilizer of the network of AEAs [15]. The results are in line with other studies conducted on peach [49,72], persimmon fruit [73], and banana [44].…”

“…By modulating SOD, POD, CAT, and the AsA-GSH cycle, CaCl 2 increased chilling tolerance in green peppers [79]. A previous study on loquat fruit found that CaCl 2 treatment increased cold tolerance by regulating energy metabolism and accumulating osmotic substances [72,80].…”

Transcriptional and Physiological Analyses to Assess the Effects of a Novel Biostimulant in Tomato

“…Our results on the lower expression levels of SODCC.1 in water-stressed plants treated with the novel biostimulant, compared to plants treated with the standard CaCl2 fertilizer, are following these last observations. Anyway, several studies on calcium chloride applications have shown the capacity of this mineral fertilizer to induce increased activities and gene expressions of superoxide dismutase and catalase which are protecting from oxidative stress produced by cold, pathogens, and drought ( Xu et al, 2013 ; Shi et al, 2014 ; Chakraborty et al, 2017 ; Hou et al, 2021 ). Nevertheless, we did not observe a significant up-regulation of superoxide dismutase following SOB02 treatments in WW conditions and the physiological results in our experiment seem to encourage the interpretation of the lower expression of SODCC.1 gene in SOB01 plants as a reduction of the water stress susceptibility of the plants.…”

Membrane Fatty Acids and Physiological Disorders in Cold-Stored ‘Golden Delicious’ Apples Treated with 1-MCP and Calcium Chloride

“…Calcium chloride was already considered as having a positive effect on ROS homeostasis in loquat fruit (Eribotrya japonica). The authors suggested that CaCl 2 treatment alleviated chilling injury through rising antioxidant enzyme activities and the ascorbate-glutathione (AsA-GSH) cycle system to scavenge ROS [41], which could, therefore, prevent the lipid peroxidation.…”