2006
DOI: 10.1080/14756360500480533
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Effects of cadmium and zinc ions on purified lamb kidney cortex glucose-6-phosphate dehydrogenase activity

Abstract: Glucose-6-phosphate dehydrogenase (G-6-PD) is the first enzyme in the pentose phosphate pathway. Cadmium is a toxic heavy metal that inhibits several enzymes. Zinc is an essential metal but overdoses of zinc have toxic effects on enzyme activities. In this study G-6-PD from lamb kidney cortex was competitively inhibited by zinc both with respect to glucose-6-phosphate (G-6-P) and NADP+ with Ki values of 1.066 +/- 0.106 and 0.111 +/- 0.007 mM respectively whereas cadmium was a non-competitive inhibitor with res… Show more

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Cited by 12 publications
(7 citation statements)
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“…Understanding the mechanism of inhibition is necessary in order to know how to regenerate the activity of the inhibited enzyme. In general, in the case of metal cations, the interaction mechanism is not completely known, but the action revealed by different metals on the activity of oxidases and dehydrogenases is usually by binding of the metal salts to the thiol groups of the proteins [26][27][28]. The affinity of Pb 2+ and Cd 2+ for SH groups and of Zn 2+ for carboxyl groups has already been reported [29].…”
Section: Repeatability Reproducibility and Stability Of The Biosensormentioning
confidence: 99%
“…Understanding the mechanism of inhibition is necessary in order to know how to regenerate the activity of the inhibited enzyme. In general, in the case of metal cations, the interaction mechanism is not completely known, but the action revealed by different metals on the activity of oxidases and dehydrogenases is usually by binding of the metal salts to the thiol groups of the proteins [26][27][28]. The affinity of Pb 2+ and Cd 2+ for SH groups and of Zn 2+ for carboxyl groups has already been reported [29].…”
Section: Repeatability Reproducibility and Stability Of The Biosensormentioning
confidence: 99%
“…Treatment with zinc and H 2 O 2 at the same time has caused higher H 2 O 2 toxicity than H 2 O 2 alone, suggesting that zinc is a direct pro-oxidant in combination with H 2 O 2 in vitro [64]. High zinc doses may be harmful on human health, because zinc can generate methemoglobin increase and oxidative stress via free radical in normal human erythrocytes, impaired immune and inflammatory responses (following an excess dose of 300 mg/day) and copper deficiency-related hemolytic anemia, because zinc may impair gastrointestinal copper absorption and inhibits G6PD activity, as observed in in vitro studies, leading to oxidative damage and consequent hemolytic anemia in G6PD deficient people [65].…”
Section: Mineralsmentioning
confidence: 99%
“…Aluminum also causes encephalopathy, anemia, and bone diseases (21). Heavy metals are generally known as enzyme inhibitors but various heavy metals, including bromine, zinc exist in the polypropylene and are categorized as endocrine disruptors and impairs thyroid hormone metabolism in the organisms (28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40). Additionally, zinc and cadmium impair mineral metabolism and parathyroid hormone function leading to the dysfunction in the endocrine metabolism in the organisms (32,41).…”
Section: Surgical Masks and Endocrine Disruptorsmentioning
confidence: 99%