Effects of Cadmium on the Activity of Matrix Metalloproteinases and Metallothionein Level in the Rat Brain

Fomenko, O. Z.; Shiyntum, Honore N.; Шевцова, А. И.; Ушакова, Г. А.

doi:10.1007/s11062-017-9645-7

Cited by 6 publications

(7 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The findings of this study are in accordance with the findings of other authors showing that Cd exposure may induce MMPs and TIMPs in various tissues and organs in the body, as well as in cell cultures [31,48,49]. On the contrary, there are also data showing that Cd may decrease the activity of MMP-2 in the prostate and testis and increase the activity of this metalloproteinase in the brain [48,50]. The lack of correlations between the liver and serum levels of MMP-1, TIMP-1, and TIMP-2 show that a measurement of the serum concentrations of these variables-unlike MMP-2, in the case of which the negative correlation between its concentration in the liver and serum occurred-cannot have predictive value regarding the MMP/TIMP status in the liver.…”

Section: Discussionsupporting

confidence: 93%

“…Only deMoura et al [36] have revealed that an eight-week intoxication with this xenobiotic had no impact on the activity of MMP-2 in rat livers. The findings of this study are in accordance with the findings of other authors showing that Cd exposure may induce MMPs and TIMPs in various tissues and organs in the body, as well as in cell cultures [31,48,49]. On the contrary, there are also data showing that Cd may decrease the activity of MMP-2 in the prostate and testis and increase the activity of this metalloproteinase in the brain [48,50].…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

The Impact of a Polyphenol-Rich Extract from the Berries of Aronia melanocarpa L. on Collagen Metabolism in the Liver: A Study in an In Vivo Model of Human Environmental Exposure to Cadmium

et al. 2020

View full text Add to dashboard Cite

This study examined whether a polyphenol-rich extract from the berries of Aronia melanocarpa L. (AE; chokeberries) may protect from the impact of cadmium (Cd) on the metabolism of collagen in the liver. The study was conducted in an experimental model (rats that were fed a diet containing 1 or 5 mg Cd/kg for 3–24 months) of human exposure to this xenobiotic during a lifetime. The concentration of total collagen and the expression of collagen types I and III at the mRNA and protein levels, as well as the concentrations of matrix metalloproteinases (MMP-1 and MMP-2) and their tissue inhibitors (TIMP-1 and TIMP-2), were assayed. The administration of Cd and/or AE had only a slight and temporary impact on the concentration of total collagen in the liver. The supplementation with AE significantly prevented Cd-mediated changes in the expression of collagen types I and III at the mRNA and protein levels and their ratio (collagen III/collagen I), as well as a rise in the concentrations of MMPs and TIMPs in this organ. The results allow the conclusion that the intake of chokeberry products in the case of Cd intoxication may be effective in prevention from this xenobiotic-induced disturbance in collagen homeostasis in the liver.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 92%

The Impact of a Polyphenol-Rich Extract from the Berries of Aronia melanocarpa L. on Collagen Metabolism in the Liver: A Study in an In Vivo Model of Human Environmental Exposure to Cadmium

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Hippocampal tissues were homogenized in a buffer solution consisting of 25 mM Tris-HCl, 1 mM EDTA, 2 mM βdithiothreitol, 0.2 mM PMSF, and 0.01% Merthiolate; pH 7,4 (100 mg of tissue/1 ml buffer) (23). Using differential ultracentrifugation, the water-soluble (containing the soluble forms of neural cell adhesion molecule-NCAM and glial fibrillary acidic protein-GFAP), membrane (containing the membrane form of NCAM), and cytoskeletal (containing the filamentous form of GFAP) protein fractions were obtained (26). We employed competitive ELISA to determine astrocyte-and neuron-specific proteins.…”

Section: Biochemical Analysis (Enzyme-linked Immunosorbent Assay)mentioning

confidence: 99%

Varying Dietary Component Ratios and Lingonberry Supplementation May Affect the Hippocampal Structure of ApoE–/– Mice

et al. 2022

View full text Add to dashboard Cite

ObjectiveThis study aimed to investigate and compare the morphological and biochemical characteristics of the hippocampus and the spatial memory of young adult ApoE–/– mice on a standard chow diet, a low-fat diet (LFD), a high-fat diet (HFD), and an HFD supplemented with lingonberries.MethodsEight-week-old ApoE–/– males were divided into five groups fed standard chow (Control), an LFD (LF), an HFD (HF), and an HFD supplemented with whole lingonberries (HF+WhLB) or the insoluble fraction of lingonberries (HF+InsLB) for 8 weeks. The hippocampal cellular structure was evaluated using light microscopy and immunohistochemistry; biochemical analysis and T-maze test were also performed. Structural synaptic plasticity was assessed using electron microscopy.ResultsApoE–/– mice fed an LFD expressed a reduction in the number of intact CA1 pyramidal neurons compared with HF+InsLB animals and the 1.6–3.8-fold higher density of hyperchromic (damaged) hippocampal neurons relative to other groups. The LF group had also morphological and biochemical indications of astrogliosis. Meanwhile, both LFD- and HFD-fed mice demonstrated moderate microglial activation and a decline in synaptic density. The consumption of lingonberry supplements significantly reduced the microglia cell area, elevated the total number of synapses and multiple synapses, and increased postsynaptic density length in the hippocampus of ApoE–/– mice, as compared to an LFD and an HFD without lingonberries.ConclusionOur results suggest that, in contrast to the inclusion of fats in a diet, increased starch amount (an LFD) and reduction of dietary fiber (an LFD/HFD) might be unfavorable for the hippocampal structure of young adult (16-week-old) male ApoE–/– mice. Lingonberries and their insoluble fraction seem to provide a neuroprotective effect on altered synaptic plasticity in ApoE–/– animals. Observed morphological changes in the hippocampus did not result in notable spatial memory decline.

show abstract

“…Cd (at a dose of 15 ppm for 20 weeks) reduces both MMP2 and MMP9 activity [ 43 ]. In another study, prolonged Cd exposure resulted in a decrease in metallothionein and MMP2 levels and an increase in the level of MMP9 [ 27 ]. In the current study, Cd decreased the serum MMP2 level although it did not change the MMP9 level.…”

Section: Discussionmentioning

confidence: 99%

Pomegranate peel extract, N-Acetylcysteine and their combination with Ornipural alleviate Cadmium-induced toxicity in rats

Korkmaz

Güngör

Demirbaş

et al. 2023

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

Cadmium is a major environmental pollutant and a highly toxic metal. It was aimed to determine the effects of pomegranate peel extract (PPE), N-acetylcysteine (NAC) alone and along with Ornipural on cadmium-induced toxicity. Forty-six Wistar Albino male rats were divided into 6 groups and the groups were formed into healthy control, Cadmium group (5 mg/kg/day, oral), Cadmium + Pomegranate peel extract (500 mg/kg, oral), Cadmium + N-acetylcysteine (100 mg/kg, oral), Cadmium + Pomegranate peel extract (500 mg/kg, oral) + Ornipural (1 mL/kg, subcutaneous) and Cadmium + N-acetylcysteine (100 mg/kg, oral) + Ornipural (1 mL/kg, subcutaneous). Cadmium accumulated heavily in both liver and kidney tissue. The administration of N-acetylcysteine and pomegranate peel extract alone reduced cadmium levels in both tissues. N-acetylcysteine treatment prevented the increase in ALT and MDA levels by cadmium damage. N-acetylcysteine + Ornipural treatment inhibited the increase in liver 8-OHdG level in the liver. N-acetylcysteine and N-acetylcysteine + Ornipural treatments prevented the reduced serum MMP2 level. N-acetylcysteine and Pomegranate peel extract + Ornipural treatments significantly reduced the increased liver iNOS level in the liver. In conclusion, NAC therapy may be a successful treatment option for cadmium toxicity. However, further research is needed on the effects of PPE and Ornipural combinations for the treatment of cadmium toxicity. In future studies, various doses of these treatment options (with chelators) should be investigated for cadmium toxicity.

show abstract

Effects of Cadmium on the Activity of Matrix Metalloproteinases and Metallothionein Level in the Rat Brain

Cited by 6 publications

References 6 publications

The Impact of a Polyphenol-Rich Extract from the Berries of Aronia melanocarpa L. on Collagen Metabolism in the Liver: A Study in an In Vivo Model of Human Environmental Exposure to Cadmium

The Impact of a Polyphenol-Rich Extract from the Berries of Aronia melanocarpa L. on Collagen Metabolism in the Liver: A Study in an In Vivo Model of Human Environmental Exposure to Cadmium

Varying Dietary Component Ratios and Lingonberry Supplementation May Affect the Hippocampal Structure of ApoE–/– Mice

Pomegranate peel extract, N-Acetylcysteine and their combination with Ornipural alleviate Cadmium-induced toxicity in rats

Contact Info

Product

Resources

About