Effects of calcitonin gene-related peptide and vasoactive intestinal peptide on nicotine-induced sweating in man

Tanaka, Etsuro; Uchiyama, S.; Nakano, Shoichi

doi:10.1016/0165-1838(90)90258-k

Cited by 15 publications

(12 citation statements)

References 14 publications

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“…Coexistence of CGRP in a subpopulation of VIP-ir cholinergic nerves to the sweat glands and in the sympathetic ganglia has been previously shown in rat (Landis and Fredieu, 1986) and cat (Lindh et al, 1988(Lindh et al, , 1989. The coexistence of VIP and CGRP is functionally significant since both have vasodilator properties (Bloom and Edwards, 1980;Brain et al, 1985) and modulate nicotine-induced sweat secretion (Tanaka et al, 1990). A direct action on the eccrine sweat gland has been proposed for VIP, as its addition to isolated sweat glands results in accumulation of CAMP in clear cells (Sato and Sato, 1987).…”

Section: Discussionmentioning

confidence: 81%

Innervation of cutaneous structures in the mouse hind paw: A confocal microscopy immunohistochemical study

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Verdú

Wendelschafer‐Crabb

et al. 1995

J of Neuroscience Research

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The normal innervation of structures in mouse foot pads was investigated with immunohistochemistry and confocal microscopy. Nerves were visualized by incubating Zamboni fixed, thick, frozen sections with antibodies to protein gene product 9.5 (PGP 9.5), vasoactive intestinal peptide, substance P, calcitonin gene-related peptide, and protein zero. The antibodies were localized using cyanine 3.18 labeled anti-rabbit gamma globulin. PGP 9.5 immunolocalization showed dense nerve bundles at the base of the foot pad with branches to larger blood vessels, sweat glands and epidermis. Sweat gland tubules were surrounded by numerous sudomotor axons; single fibers accompanied the sweat duct toward the skin's surface. Nerve bundles containing myelinated and unmyelinated axons ran through and around the centrally located sweat gland cluster to end in free nerve endings and Meissner's-like corpuscles at the apex of the foot pad. Other bundles running parallel to the epidermis gave arcuate branches that supplied epidermis on the sides of the pads with a rich nerve network, principally with free nerve endings that often reached the most superficial cell layers of epidermis. Calcitonin gene-related peptide-immunoreactive (-ir) nerves were distributed to dermis and epidermis in lower density than PGP 9.5-ir fibers. Substance P-ir fibers were less numerous; most terminated as free endings in deeper layers of epidermis. Vasoactive intestinal peptide-ir nerves almost exclusively innervated sweat glands, ducts and blood vessels, but not epidermis. The mouse hind paw has potential to serve as a model system for investigations of functional and morphological changes that affect peripheral and autonomic nerves under diverse experimental conditions.

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Section: Discussionmentioning

confidence: 81%

Innervation of cutaneous structures in the mouse hind paw: A confocal microscopy immunohistochemical study

Navarro

Verdú

Wendelschafer‐Crabb

et al. 1995

J of Neuroscience Research

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“…Similarly, SP has vasodilator effects (Pernow, 1983) and is often found to colocalise with CGRP in nerves. Moreover, it has been reported that CGRP (and VIP) exert an influence on human sweating under physiological conditions (Tanaka et al 1990). Our findings therefore suggest that CGRP and SP-containing cells are involved in the local vasodilation associated with increased sweat production as well as in regulation of the contractile activity of myoepithelial cells, e.g.…”

Section: mentioning

confidence: 99%

Immunohistochemical evidence suggests intrinsic regulatory activity of human eccrine sweat glands

et al. 1999

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Immunohistochemistry of normal eccrine sweat glands was performed on paraffin sections of human skin. Immunoreactivity (ir) for neuron specific enolase, S100 protein (S100), regulatory peptides, nitric oxide synthase type I (NOS-I) and choline-acetyltransferase (ChAT) was found in small nerve bundles close to sweat glands. In the glands, secretory cells were labelled with anticytokeratin antibody. Using antibodies to S100, calcitonin gene-related peptide (CGRP) and substance P (SP) a specific distribution pattern was found in secretory cells. Granulated (dark) and parietal (clear) cells were immunopositive for CGRP, and S100 and SP, respectively. Immunoreactivity was diffuse in the cytoplasm for CGRP and S100, and peripheral for SP. Myoepithelial cells were not labelled. Electron microscopy revealed electron dense granules, probably containing peptide, in granulated cells. Using antibodies to NOS-I and ChAT, ir was exclusively found in myoepithelial cells. Immunoreactivity for the atrial natriuretic peptide was absent in sweat glands. These results provide evidence for the presence of both regulatory peptides involved in vasodilation and key enzymes for the synthesis of nitric oxide and acetylcholine in the secretory coil of human sweat glands. It is suggested that human sweat glands are capable of some intrinsic regulation in addition to that carried out by their nerve supply.Key words : Neuropeptides ; nitric oxide synthase ; choline-acetyltransferase ; substance P ; calcitonin gene-related peptide. Eccrine sweat glands are widespread in human skin and represent a major source of evaporative heat loss from the body. They consist of a secretory coil located deep in the reticular layer of the dermis or in the hypodermis and a duct reaching the skin surface. Three cell types are consistently described in the secretory coil of eccrine sweat glands namely, the ' clear ', ' dark ', and myoepithelial cells (Ellis, 1967 ; Breathnach, 1971). These cell types have been associated with water and ion secretion, production of sweat proteins, and the mechanism of sweat transfer to the excretory duct, respectively (Kurosumi et al. 1984 ;Sato et al. 1989). Typically, myoepithelial cells are placed in a basal position in the epithelium, possess a few, long cell processes running on the basal lamina, and they do not reach the lumen. In contrast,Correspondence to Dr Carlo Zancanaro, Istituto di Anatomia Umana ed Istologia, Strada Le Grazie, 8, I-37134 Verona, Italy. Tel. : j39-45-8098155 ; fax j39-45-8098163 ; e-mail : Carloz!borgoroma.univr.it both clear and dark cells contact the lumen. Preliminary light and electron microscopic investigations on human sweat glands showed that most clear cells are flask-shaped and are placed in a parietal position in the secretory epithelium ; their contact with the lumen is mainly by means of intra and intercellular secretory canaliculi provided with short microvilli. Dark cells consistently show extensive, direct contact with the lumen and thin basal processes. On the ba...

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“…of this neuropeptide on exocrine glands (including the main lacrimal gland of a variety of species) has been demonstrated by a number of researchers (Lundberg et al, 1980 ;Dartt et al, 1984 ;Stolze and Sommer, 1985 ;Tanaka et al, 1990). In addition, the mucine secretion of the goblet cells of the bulbar conjunctiva of rats has been stimulated by VIP both in vivo and in vitro (Kessler and Dartt, 1994 ;Dartt et al, 1996).…”

Section: Discussionmentioning

confidence: 94%

Vasoactive Intestinal Polypeptide (VIP) Innervation of the Human Eyelid Glands

Seifert

Spitznas

1999

Experimental Eye Research

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Effects of calcitonin gene-related peptide and vasoactive intestinal peptide on nicotine-induced sweating in man

Cited by 15 publications

References 14 publications

Innervation of cutaneous structures in the mouse hind paw: A confocal microscopy immunohistochemical study

Innervation of cutaneous structures in the mouse hind paw: A confocal microscopy immunohistochemical study

Immunohistochemical evidence suggests intrinsic regulatory activity of human eccrine sweat glands

Vasoactive Intestinal Polypeptide (VIP) Innervation of the Human Eyelid Glands

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