Effects of calcitonin on elemental distribution and ultrastructure of rat submandibular gland acinar cells

Sagulin, G.-B.; Wróblewski, J; Arver, Stefan; Roomans, Godfried M.

doi:10.1111/j.1748-1716.1985.tb07767.x

Cited by 6 publications

(5 citation statements)

References 14 publications

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“…T h e CT-induced increase in total cellular calcium levels in submandibular acinar cells (Sagulin et al 1985) is not in contradiction with the present findings that CT does not affect 1987). affect the calcium/protein ratio in the final saliva.…”

Section: Measurements Of [Ca'+]contrasting

confidence: 60%

“…In a previous study we found that C T causes accumulation of mucus in rat submandibular acinar cells (Sagulin et al 1985). It appears reasonable to suggest that the inhibition of cAMP production in the glands by C T would be at least partly responsible for the observed inhibition of mucus secretion.…”

Section: Measurements Of [Ca'+]mentioning

confidence: 89%

“…D reportedly increase the calcium concentrations in human ( Schneider et al 1977, Maier et al 1980 and rat saliva (Glijer et al 1985). Our investigation on the effect of calcitonin (CT) on rat submandibular gland showed an increase in the content of mucus, total calcium and magnesium in the acinar cells (Sagulin et al 1985). Hypertrophy (Becker et al 1984, Wolf et a/.…”

mentioning

confidence: 80%

“…In an earlier study we found that C T reduced the response to pilocarpine stimulation in the acinar cells of rat submandibular gland. Pilocarpine stimulation is associated with an increase of the intracellular Na/K ratio, and this increase was reduced in the presence of C T (Sagulin et al 1985). Therefore, the acinar cells rather than the duct cells appear to be the site of action G.-B.…”

Section: Measurements Of [Ca'+]mentioning

confidence: 96%

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Mechanism of action of calcitonin on secretion in rat submandibular gland

Sagulin

Müller²,

Westlind‐Danielsson

et al. 1989

Acta Physiologica Scandinavica

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Calcitonin (CT) was found to reduce the initial flow of pilocarpine-stimulated saliva from the submandibular glands in the rat. Although there was a concomitant increase of the concentration of calcium and protein in the saliva, the calcium/protein ratio was not significantly affected. CT also caused a significant increase of the potassium concentration in submandibular saliva. Both in vivo and in vitro, CT inhibited the production of cyclic AMP (cAMP) both in the absence and in the presence of forskolin. This decrease in intracellular cAMP levels could result in an inhibition of mucus secretion, which would explain the previously observed calcitonin-induced intracellular accumulation of mucus in the submandibular gland acinar cells. CT did not affect the cytoplasmic free Ca2+ concentration (as measured with fura 2) in isolated submandibular acini either in the absence or in the presence of cholinergic or adrenergic agonists. These results indicate that the inhibition of fluid secretion in the submandibular gland by calcitonin must be located distal to changes in [Ca2+]i. It can be concluded that CT affects both mucus and fluid secretion in the submandibular gland, but that only the inhibition of mucus secretion can as yet be explained by an effect at the level of the second messenger.

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Section: Measurements Of [Ca'+]contrasting

confidence: 60%

Section: Measurements Of [Ca'+]mentioning

confidence: 89%

mentioning

confidence: 80%

Section: Measurements Of [Ca'+]mentioning

confidence: 96%

See 2 more Smart Citations

Mechanism of action of calcitonin on secretion in rat submandibular gland

Sagulin

Müller²,

Westlind‐Danielsson

et al. 1989

Acta Physiologica Scandinavica

View full text Add to dashboard Cite

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“…Since the concentration of calcium in the mucus granules is much higher thar in any other cell compartment, the cellular calcium concentration sr 9b mandibular acinar cells is largely determined by the relative volume of intracellular mucus (Roomans, 1986). Accumulation of intracellular mucus is also seen after treatment wit reserpine, and after chronic treatment with isoproterenol and other beta adrenergic agonists (Muller and Roomans, 1985), and with furosemide (Scarlett et at, 1988), and caln he induced by a single dose of alloxan (Sagsrrom et 19a,197) and calcilonin (Sagulin et at, 1985). In general, mucu:s accumulation appears to be associated with a reduced response to beta: adrenergic stimulation of low to moderate intensity, but the molecular basis of this reduction may be different for the var ious treatments.…”

Section: Discussionmentioning

confidence: 95%

Effects of Thyroxine and Dexamethasone on Rat Submandibular Glands

Sagulin

Roomans

1989

J Dent Res

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Glucocorticoids and thyroxine are known to have a marked effect on the flow rate and protein composition of rat parotid saliva in hormonally intact animals. In the present study, the effects of a one-week treatment of male rats with dexamethasone and thyroxine were studied by electron microscopy and x-ray micro-analysis, and by measurement of the flow rate and determination of the chemical composition of pilocarpine-induced submandibular saliva. Thyroxine had the most extensive effects on the submandibular gland. The acinar cells were enlarged and filled with mucus; the cellular calcium concentration was significantly increased. The flow rate of the submandibular saliva was significantly reduced compared with that in saline-injected control animals. Thyroxine caused an increase in the concentrations of protein, total calcium, and potassium in the saliva. Dexamethasone had no significant effects on gland ultrastructure or on the elemental composition of the acinar cells; flow rate was not affected, but the concentrations of protein, calcium, and potassium were significantly increased. The effects of dexamethasone and thyroxine on the flow rate and protein composition of pilocarpine-induced rat submandibular saliva differ from those reported earlier for rat parotid saliva after simultaneous stimulation with pilocarpine and isoproterenol.

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