Effects of calcitonin on transport and intracellular distribution of exchangeable Ca2+ in primary culture of bone cells

Eilam, Y.; Szydel, N.; Harell, A

doi:10.1016/0303-7207(80)90067-2

Cited by 16 publications

(9 citation statements)

References 18 publications

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“…In kidney cells this increase in 4s Ca uptake reflects only an increased intracellular exchangeable calcium pool and not an increased influx because the steady-state calcium exchange across the plasma membrane is actually depressed by calcitonin (Borle 1975a). In agreement with this latter finding, 4s Ca efflux is depressed by calcitonin in kidney cells and in bone cells (Borle 1969c(Borle , 1975cHarell et al 1973;Eilam 1980). The increased cellular calcium appears to be sequestered in a cellular pool identified with mitochondria (Borle 1973a(Borle , 1975aEilam 1980).…”

Section: Calcitoninsupporting

confidence: 63%

“…Meer 1974;Dziak and Stern 1975Deshmukh et al 1977Wattington et al 1968 BorIe 1969, c Yamaguchi et al 1975Yamaguchi 1979Harrell et al 1973Binderman et al 1974;Eilam et al 1980 Glucose Pancreas Carafoli and Crovetti 1973 ;Carafoli 1975 Rodan andRodan 1976;Gerrard et al 1977Rasmussen et al 1975Sehrier et al 1975 Wollheim et al 1978;Gylfe et al 1978;Tsumura et al 1979;Hersdmetz et al 1979Hersdmetz et al , 1980Hersehnelz and Mataisse 1980 13.1 Cyclic AMP Most peptide hormones bind to specific receptors on the membrane of their target cells, stimulate the enzyme adenylate cyclase, and increase the intracellular concentration of cyclic AMP. Several other cyclic nucleotides such as cyclic GMP are also affected and there may be complex interactions among cyclic AMP, cyclic GMP, and calcium.…”

Section: Kiiser-glanzman 1977mentioning

confidence: 99%

“…Calcitonin increases the total cellular calcium in kidney cells (Borle 1969, t 975a, c), in bone cells (Eilam et al 1980), and in liver (Yamaguchi et al 1975;Yamaguchi 1979). It also stimulates the uptake of 4SCa in kidney cells (Borle 1969c(Borle , 1975a, bone cells (Harell et al 1973(Harell et al , 1975Binderman et al 1974;Eilam 1980), and chondrocytes (Deshmukh et al 1977).…”

Section: Calcitoninmentioning

confidence: 99%

“…It also stimulates the uptake of 4SCa in kidney cells (Borle 1969c(Borle , 1975a, bone cells (Harell et al 1973(Harell et al , 1975Binderman et al 1974;Eilam 1980), and chondrocytes (Deshmukh et al 1977). In kidney cells this increase in 4s Ca uptake reflects only an increased intracellular exchangeable calcium pool and not an increased influx because the steady-state calcium exchange across the plasma membrane is actually depressed by calcitonin (Borle 1975a).…”

Section: Calcitoninmentioning

confidence: 99%

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Control, modulation, and regulation of cell calcium

Borle

1981

Reviews of Physiology, Biochemistry and Pharmacology

299

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Section: Calcitoninsupporting

confidence: 63%

Section: Kiiser-glanzman 1977mentioning

confidence: 99%

Section: Calcitoninmentioning

confidence: 99%

Section: Calcitoninmentioning

confidence: 99%

See 2 more Smart Citations

Control, modulation, and regulation of cell calcium

Borle

1981

Reviews of Physiology, Biochemistry and Pharmacology

299

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“…The rate of calcium influx into cells was measured by adding 45Ca to the cultures for 3 min [ 18]. Control cultures using lanthanum nitrate indicated a 90% inhibition of calcium uptake.…”

Section: Resultsmentioning

confidence: 99%

Elevated concentrations of synthetic fluorinated glucocorticoid analogs transiently increase the intracellular exchangeable calcium in cultured bone cells

et al. 1982

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Summary. This study investigated the influence of various glucocorticoids on the transport and accumulation of Ca 2+ in cultured bone cells. For measuring changes in the amount of intracellular exchangeable Ca 2+, cultures were initially preincubated with 4~Ca for 48 h thereby achieving a steady state. Triamcinolone acetonide induced a transient increase in the cells' content of exchangeable Ca2+--an effect that lasted for 5 h and was followed by a pronounced decrease as noted at 24 h. A similar increase was observed with dexamethasone, whereas hydrocortisone and corticosterone led to a less significant effect. No significant changes took place with the use of deoxycorticosterone, progesterone, and estradiol. The effect of triamcinolone on the cellular content of exchangeable Ca 2+ was completely blocked by both cycloheximide and puromycin when added shortly after the addition of the corticosteroid to the culture system. In order to determine the effect of steroid hormones on the initial rate of Ca 2+ influx into cultured cells, cultures were first preincubated with the various hormones and thereafter 45Ca was added. Only fluorinated glucocorticoid analogs such as triamcinolone acetonide and dexamethasone induced significant rises in the initial rate of Ca 2+ influx.Ultrastructural examinations showed that in 5-day-old control cultures osteoblast-like cells show multiple aggregates of calcium pyroantimonate along their plasma membrane. In contrast, similar cells cultured in the presence of triamcinolone (10-TM) for 3 h lacked such precipitates along their plasma membrane but instead contained aggregates of calcium pyroantimonate within enlarged mitochondria. Bone cells that were incubated with Send offprint requests to Dr. Michael Silbermann at the above address.triamcinolone for a longer period of time (24 h) exhibited hypertrophied mitochondria that were devoid of such calcium precipitates. Hence this study indicates that potent synthetic analogs of glucocorticoids affect: (a) the rate of calcium influx into bone cells, (b) the intracellular concentration of calcium, and (c) the distribution of calcium within these cells.

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Human bone cell cultures: A new model for studying the mechanism of action of calcitonin

Fanò

Maurizi

Venti-Donti

et al. 1985

Cell Biochemistry & Function

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An investigation on cell cultures obtained from temporal human bone fragments showed that they provide a suitable model for studying the mechanism involved in calcitonin action on bone cells. Furthermore they demonstrated: a transitory increase in 45Ca uptake that returned to control values ten minutes after the hormone was added; a relation between 45Ca uptake and increased cAMP concentrations when these were measured at the same time intervals; a reproduction of the salmon calcitonin (sCT) effect after incubation of the cultures with either db-cAMP or db-cGMP and inhibition of 45Ca uptake and parallel decrease in cAMP levels with propanol. These results suggest that in human bone cell cultures, sCT acts as a temporary promoter of 45Ca uptake, probably by activating an adenylate-cyclase system through a beta-receptor.

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Effects of calcitonin on transport and intracellular distribution of exchangeable Ca2+ in primary culture of bone cells

Cited by 16 publications

References 18 publications

Control, modulation, and regulation of cell calcium

Control, modulation, and regulation of cell calcium

Elevated concentrations of synthetic fluorinated glucocorticoid analogs transiently increase the intracellular exchangeable calcium in cultured bone cells

Human bone cell cultures: A new model for studying the mechanism of action of calcitonin

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