Effects of camptothecin and hydroxycamptothecin on insect cell lines Sf21 and IOZCAS‐Spex‐II

Zhang, Lan; Zhang, Yanning; He, Weizhi; Ma, Dejun; Jiang, Hongyun

doi:10.1002/ps.2313

Cited by 35 publications

(21 citation statements)

References 36 publications

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“…In all experiments, IOZCAS-Spex-II cells were pretreated with CPT and HCPT according to the procedure described in our previous studies [16]. The Topo1 was extracted from IOZCAS-Spex-II cells, and the specific activity of the nuclear extract in each sample was determined using the method described above.…”

Section: Methodsmentioning

confidence: 99%

“…To find new CPT-derived insecticides with improved efficacy and to determine the potential structural factors required for the biological activity of CPT analogues, a series of novel CPT derivatives have been semi-synthesized, and some of them were found toxic to armyworms ( Mythimna separate Walker) and coconut palm beetles ( Brontispa longissima Gestro) [14], [15]. In our previous studies, we found that 0.1–30 µM CPT treatment to IOZCAS -Spex-II cells (established from beet armyworms, Spodoptera exigua Hübner) induce cell apoptosis [16]. Thus, these previous findings intrigue us to explore CPT as a lead compound of an insect control agent, and to investigate the possibility of exploring a new Topo1-targeted botanical insecticide.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of DNA Topoisomerase-1 in Spodoptera exigua for Toxicity Evaluation of Camptothecin and Hydoxy-Camptothecin

Zhang

et al. 2013

PLoS ONE

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Camptothecin (CPT), a plant alkaloid originally isolated from the native Chinese tree, Camptotheca acuminate, exerts the toxic effect by targeting eukaryotic DNA topoisomerase 1 (DNA Topo1). Besides as potent anti-cancer agents, CPT and its derivatives are now being explored as potential pesticides for insect control. In this study, we assessed their toxicity to an insect homolog, the Topo1 protein from beet armyworms (Spodoptera exigua Hübner), a worldwide pest of many important crops. The S. exigua Topo1 gene contains an ORF of 2790 base pairs that is predicted to encode a polypeptide of 930 amino acids. The deduced polypeptide exhibits polymorphism at residue sites V420, L530, A653 and T729 (numbered according to human Topo1) among insect species, which are predicted to confer sensitivity to CPT. The DNA relaxation activity of this protein was subsequently examined using a truncated form that contained the residues 337–930 and was expressed in bacteria BL21 cells. The purified protein retained the ability to relax double-stranded DNA and was susceptible to CPT and its derivative hydroxy-camptothecin (HCPT) in a dose-dependent manner. The same inhibitory effect was also found on the native Topo1 extracted from IOZCAS-Spex-II cells, a cell line established from beet armyworms. Additionally, CPT and HCPT treatment reduced the steady accumulation of Topo1 protein despite the increased mRNA expression in response to the treatment. Our studies provide information of the S. exigua Topo1 gene and its amino acid polymorphism in insects and uncover some clues about potential mechanisms of CPT toxicity against insect pests. These results also are useful for development of more effective Topo1-targeted CPT insecticides in the future.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of DNA Topoisomerase-1 in Spodoptera exigua for Toxicity Evaluation of Camptothecin and Hydoxy-Camptothecin

Zhang

et al. 2013

PLoS ONE

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“…Consistent with these results, Zhang et al recently revealed that CPT caused Sf21 and IOZCAS‐Spex‐II cell apoptosis via a mitochondrial‐dependent apoptosis signal transduction pathway, suggesting that its mode of action may be related to apoptosis. Moreover, pretreatment with CPT led to reduction in both the enzymatic activity and the steady accumulation of the topo I protein in IOZCAS‐Spex‐II cells despite upregulation of its mRNA expression in response to the treatment . In connection with these efforts, in order to find new CPT‐derived insecticides with improved profiles and to clarify the SARs of campothecin analogs, a number of CPT derivatives modified in the different positions have been synthesized and evaluated for their insecticidal activity against some important insect pests by our group; among which, some compounds exhibited insecticidal activity equal to or higher than that of CPT.…”

Section: Biological Activity Of Cpt and Related Derivativesmentioning

confidence: 99%

Perspectives on Biologically Active Camptothecin Derivatives

et al. 2015

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Camptothecins (CPTs) are cytotoxic natural alkaloids that specifically target DNA topoisomerase I. Research on CPTs has undergone a significant evolution from the initial discovery of CPT in the late 1960s through the study of synthetic small molecule derivatives to investigation of macromolecular constructs and formulations. Over the past years, intensive medicinal chemistry efforts have generated numerous CPT derivatives. Three derivatives, topotecan, irinotecan, and belotecan, are currently prescribed as anticancer drugs, and several related compounds are now in clinical trials. Interest in other biological effects, besides anticancer activity, of CPTs is also growing exponentially, as indicated by the large number of publications on the subject during the last decades. Therefore, the main focus of the present review is to provide an ample but condensed overview on various biological activities of CPT derivatives, in addition to continued up-to-date coverage of anticancer effects.

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“…Consistent with these data, Zhang et al . have reported that CPT caused Sf21 and IOZCAS‐Spex‐II cell apoptosis via a mitochondrial‐dependent apoptosis signal transduction pathway …”

Section: Discussionmentioning

confidence: 99%

“…However, the mechanism of action of CPT as a botanical pesticide is still unknown. Recent observations showed that CPT could induce apoptosis in different insect cell lines, suggesting that its mode of action may be related to apoptosis …”

Section: Introductionmentioning

confidence: 99%

Camptothecin‐induced expression of programmed cell death gene 11 in Spodoptera litura

Gong¹,

Wang²,

Huang³

et al. 2013

Pest Management Science

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Effects of camptothecin and hydroxycamptothecin on insect cell lines Sf21 and IOZCAS‐Spex‐II

Cited by 35 publications

References 36 publications

Characterization of DNA Topoisomerase-1 in Spodoptera exigua for Toxicity Evaluation of Camptothecin and Hydoxy-Camptothecin

Characterization of DNA Topoisomerase-1 in Spodoptera exigua for Toxicity Evaluation of Camptothecin and Hydoxy-Camptothecin

Perspectives on Biologically Active Camptothecin Derivatives

Camptothecin‐induced expression of programmed cell death gene 11 in Spodoptera litura

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