Effects of carbon doping on the microstructural, micro/nano-mechanical, and mesenchymal stromal cells biocompatibility and osteogenic differentiation properties of alumina

Krishnamurithy, G.; Yahya, Noor Azlin; Mehrali, Mehdi; Sadeghinezhad, Emad; Mohan, Saktiswaren; Murali, Malliga Raman; Raghavendran, Hanumantha Rao Balaji; Kamarul, Tunku

doi:10.1016/j.ceramint.2016.08.148

Cited by 9 publications

(13 citation statements)

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“…This protein is produced and secreted primarily by osteoblasts in the process of mineralization [36]. OC is therefore well known as late stage osteoblastic differentiation protein and it is also considered osteoblastic-specific protein [4]. In the present study, the OC secretion at different time points was demonstrated using OC Elisa assay (Fig 7B).…”

Section: Resultssupporting

confidence: 49%

“…Biomechanical properties are crucial characteristics for ceramics, especially when abrasive or grinding action is required. In general, compressive strength is related to crystal structure, bonding, grain size and density, however can be affected by secondary phases, grain boundaries residual stress and impurities [4]. Fig 4 shows a typical stress-strain response following compression of the FU scaffold.…”

Section: Resultsmentioning

confidence: 99%

“…Informed consent was obtained prior to acquiring samples. The hBMSCs isolation methods were adopted from our published article [4]. Briefly, bone marrow mononucleated cells (MNCs) were purified using standard Ficoll-Pague gradient centrifugation (density 1.073 g/mL) according to manufacturer’s instruction (GE Healthcare Bio-Sciences, USA).…”

Section: Methodsmentioning

confidence: 99%

“…For total cellular fluorescence calculation, the confocal images were further processed using Image-J analysis software (IJ 151j/Java 1.8.2-64-bit, NIH, USA). Three random regions of interest (ROIs) were assigned for each interrogation and the corrected total cell fluorescence (CTCF) was calculated using the following equation: CTCF integrated density—(area of selected ROI × fluorescence of background reading) [4]. Data were presented as mean ± SD.…”

Section: Methodsmentioning

confidence: 99%

“…The inherent characteristics such as biocompatibility, bioinert and durability of these materials allow them to be as a primary choice in engineering orthopaedic or orthodontic implants [2, 3]. To date, bio-ceramics such as alumina, zirconia, silicate, and phosphate ceramics have been gaining much attention among the ceramists [4]. However, certain limitations associated with these bio-ceramics such as the intrinsic brittleness, poor wear resistance and fracture toughness restrict their wide-ranging applications for the treatment of bone defects [5].…”

Section: Introductionmentioning

confidence: 99%

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The physicochemical and biomechanical profile of forsterite and its osteogenic potential of mesenchymal stromal cells

et al. 2019

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It has been demonstrated that nanocrystalline forsterite powder synthesised using urea as a fuel in sol-gel combustion method had produced a pure forsterite (FU) and possessed superior bioactive characteristics such as bone apatite formation and antibacterial properties. In the present study, 3D-scaffold was fabricated using nanocrystalline forsterite powder in polymer sponge method. The FU scaffold was used in investigating the physicochemical, biomechanics, cell attachment, in vitro biocompatibility and osteogenic differentiation properties. For physicochemical characterisation, Fourier-transform infrared spectroscopy (FTIR), Energy dispersive X-ray (EDX), X-ray diffraction (XRD), Raman spectroscopy, X-ray photoemission spectrometer (XPS) and Brunauer-Emmett-Teller (BET) were used. FTIR, EDX, XRD peaks and Raman spectroscopy demonstrated correlating to FU. The XPS confirmed the surface chemistry associating to FU. The BET revealed FU scaffold surface area of 12.67 m 2 /g and total pore size of 0.03 cm 3 /g. Compressive strength of the FU scaffold was found to be 27.18 ± 13.4 MPa. The human bone marrow derived mesenchymal stromal cells (hBMSCs) characterisation prior to perform seeding on FU scaffold verified the stromal cell phenotypic and lineage commitments. SEM, confocal images and presto blue viability assay suggested good cell attachment and proliferation of hBMSCs on FU scaffold and comparable to a commercial bone substitutes (cBS). Osteogenic proteins and gene expression from day 7 onward indicated FU scaffold had a significant osteogenic potential ( p <0.05), when compared with day 1 as well as between FU and cBS. These findings suggest that FU scaffold has a greater potential for use in orthopaedic and/or orthodontic applications.

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Section: Resultssupporting

confidence: 49%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%