Effects of Carob (Ceratonia siliqua) on Sperm Quality, Testicular Structure, Testosterone Level and Oxidative Stress in Busulfan-Induced Infertile Mice

Vafaei, Azadeh; Mohammadi, Shabnam; Fazel, Alireza; Soukhtanloo, Mohammad; Mohammadipour, Abbas; Beheshti, Farimah

doi:10.15171/ps.2018.16

Cited by 29 publications

(23 citation statements)

References 19 publications

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“…Then, 65 µL of phosphate buffered saline, 30 µL (MTT 1.25 mmoll), and 75 µL of pyrogallel (100 µmoll) were mixed with 10 µL of tissues, and incubated for several minutes at room temperature. After that, 75 µL dimethyl sulfoxide were added and absorption was read at 570 nm wavelength, using ELISA reader [17].…”

Section: The Level Of Superoxide Dismutasementioning

confidence: 99%

The Effect of Propranolol on Sperm Parameters, CatSper 2 Gene and Protein Expression, and Oxidative Stress in Adult Mice

Mohammadi

Beheshti

Elyasi

et al. 2020

JPRI

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The aim of this study was to investigate the effects of propranolol on sperms, histopathology of testes, and CatSper 2 gene and protein expression in adult mice. 18 adult male mice were randomly divided into control, propranolol 1 (receiving 10 mg/kg dose) and propranolol 2 group (receiving 15 mg/kg dose for 35 days). The mean amount of sperm parameters in the propranolol 1 and propranolol 2 groups was significantly lower than the control group (p<0.05). CatSper2 gene and protein expression have significantly decreased in propranolol 1 and propranolol 2 groups compared to the control group (P<0.05). Reduction of CatSper2 gene and protein expression in low dose of propranolol was more severe than high dose. In testicular tissues of the propranolol 1 group, vacuoles and necrosis in the germinal epithelium were observed, and in testicular tissues of propranolol 2 group decrease in the thickness of the germinal epithelium, some vacuoles and necrosis were observed in germinal epithelium as well as congestion in the interstitial space. The mean value of thiol and catalase enzyme in the propranolol 1 and propranolol 2 groups, and the mean value of superoxide dismutase in propranolol 1 group, were significantly different compared to the control group (P<0.05).

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Section: The Level Of Superoxide Dismutasementioning

confidence: 99%

The Effect of Propranolol on Sperm Parameters, CatSper 2 Gene and Protein Expression, and Oxidative Stress in Adult Mice

Mohammadi

Beheshti

Elyasi

et al. 2020

JPRI

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“…The carob 1-3 groups intraperitoneally received 200 mg/kg, 400 mg/kg, and 800 mg/kg of carob for 14 days. 12 Then, the amount of testicular apoptosis, sperm analysis, oxidative stress, and weight organ indexes were evaluated on Day 35 of the experiment.…”

Section: Animal and Ethical Issuesmentioning

confidence: 99%

“…To measure the thiol level, a di-nitrobenzoic acid reagent was used and absorption at 412 nm was investigated by using a spectrophotometer. 12 To measure the level of malondialdehyde, the homogenous tissue of the testis was mixed with chlorideric acid and placed in a water bath. After cooling, centrifugation, and absorption at 535 nm, the level was read by the spectrophotometer.…”

Section: Measurement Of Thiol and Malondialdehyde Levelsmentioning

confidence: 99%

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Effects of Carob Fruit Extract on Spermatogenesis, Antioxidant Status, and Apoptosis in Adult Male Mice

et al. 2019

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Background: Certain plants stimulate spermatogenesis and increase fertility; in contrast, some plants arrest the spermatogenesis cycle. Ceratonia siliqua is an herb plant with a strong antioxidant property. The aim of this study was to evaluate the effects of carob fruit extract on spermatogenesis, testicular apoptosis, and oxidative stress in adult male mice. Methods: Forty adult male mice were randomly divided into five groups: control, sham, and carob 1–3. The sham group was injected with normal saline and the carob 1–3 groups were injected with 200, 400, and 800 mg/kg of the carob fruit extract intraperitoneally for 14 days, respectively. At the end of the injection period, spermatogenesis, testicular apoptosis, and oxidative stress were examined. Results: The sperm parameters increased in the mice that received 200 mg/kg of carob compared to the sham group (p <0.05). There was a significant increase in the weight index of the epididymis in the carob 3 group in comparison to the sham group (p = 0.01). The number of positive tunnel cells was not statistically significant between different groups (p>0.05). The level of malondialdehyde decreased in the carob 1 and carob 3 groups, but this reduction was not statistically significant (p > 0.05). In addition, the statistical analysis showed a significant difference in the mean superoxide dismutase levels in the carob 2 and carob 3 groups in comparison to the sham group (p ≤ 0.001). The statistical analysis showed a significant increase in the mean level of the catalase enzyme in the carob 1 group in comparison to the sham (p = 0.02), and carob 2 groups (p = 0.008). Conclusion: The administration of 200 mg of the carob fruit extract for 14 days increased the testicular index as well as sperm parameters and decreased the level of oxidative stress in the testicular tissue of adult mice.

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“…It contains carbohydrates (40%), fat (1%), protein (4%), vitamins (E, C, D, B6, niacin), folic acid, polyphenol, calcium, iron, potassium, sodium and phosphorus (Aghajani, Mahdinezhad Gorji, Mirabi, & Mojab, 2019). There are studies that suggest that carob can provide protective effect against oxidative stress products in various organs via its antioxidant activity, while having no toxic effects (Aghajani et al., 2019; Gulay, Yildiz‐Gul, Ata, Balic, & Demirtas, 2012; Soleimanzadeh, Kian, Moradi, & Mahmoudi, 2020; Vafaei et al, 2018). Although it has been used traditionally with a consideration that it has a positive effect on semen parameters, there are not enough studies investigating its intratesticular effects in the presence of oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

Carob attenuates nicotine‐induced oxidative stress and intratesticular damage in male rats

Öztekin

Caniklioğlu

Fırat³

et al. 2020

Andrologia

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Infertility is a social health problem that affects approximately 1/7 of the population in the reproductive age worldwide and can cause financial and emotional stress (Sarac & Koc, 2018). Semen pathologies and male-related factors appear in about half of the couples who have infertility (Aston, Krausz, Laface, Ruiz-Castane, & Carrell, 2010). Negative effects of the diet, lifestyle, environmental factors, medications and chronic exposure to toxic substances in individuals with infertility have been reported in public health studies (Gabrielsen & Tanrikut, 2016). Smoking and nicotine exposure is a common social habit that can cause serious health problems. Nicotine is a primary component that causes smoking addiction. It is promptly absorbed in the respiratory tract mucosa and its level rises rapidly in the blood circulation and the brain (Motaghinejad, Motevalian, Fatima, Faraji, & Mozaffari, 2017). In addition to being involved in the aetiology of cardiovascular diseases and cancer, nicotine has been shown to have negative effects on genetic, immunological and reproductive health. It is thought that these negative effects are caused by disruption in oxidant-antioxidant balance (Saad, Rjeibi, Brahmi, Elaloui, & Zouari, 2019). It also has been emphasised that the disruption of antioxidant defence

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Effects of Carob (Ceratonia siliqua) on Sperm Quality, Testicular Structure, Testosterone Level and Oxidative Stress in Busulfan-Induced Infertile Mice

Cited by 29 publications

References 19 publications

The Effect of Propranolol on Sperm Parameters, CatSper 2 Gene and Protein Expression, and Oxidative Stress in Adult Mice

The Effect of Propranolol on Sperm Parameters, CatSper 2 Gene and Protein Expression, and Oxidative Stress in Adult Mice

Effects of Carob Fruit Extract on Spermatogenesis, Antioxidant Status, and Apoptosis in Adult Male Mice

Carob attenuates nicotine‐induced oxidative stress and intratesticular damage in male rats

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