Effects of carrot puree with enhanced levels of chlorogenic acid on rat cognitive abilities and neural development

Abstract

2020)Effects of carrot puree with enhanced levels of chlorogenic acid on rat cognitive abilities and neural development, ABSTRACT In this study, the effect of carrot puree with enhanced levels of chlorogenic acid, obtained from carrots treated with wounding stress, on Lactobacillus concentration in microbiota, cognitive and brain development in two generations of rats was evaluated. Wistar rats were randomly assigned three different diets: control, 90% (w/w) control + 10% carrot puree (CP), and 90% control + 10% wounding stress carrot puree (WSCP). Wounding stress enhanced chlorogenic acid concentration~4 times when compared to untreated CP (522 mg/kg). Rats treated with WSCP and CP diets increased the counts of Lactobacillus in the gut microbiota as compared with the control group (p ˂ 0.019) of the second generation. Myelin content of WSPC was significantly higher than control and CP groups in the first generation. In the second generation, myelin raised to 204 mg/g in WSCP group and this group had the highest RNA content. Overall, results indicate that WSCP improves brain development by increasing myelin concentration and RNA.Efectos del puré de zanahoria con niveles elevados de ácido clorogénico sobre las capacidades cognitivas y desarrollo neuronal de ratas RESUMEN En este estudio, se evaluó el efecto del puré de zanahoria con niveles elevados de ácido clorogénico, obtenido de zanahorias tratadas con estrés de corte, sobre la concentración de Lactobacillus en la microbiota y el desarrollo cognitivo y cerebral de dos generaciones de ratas. Las ratas Wistar se dividieron de forma aleatoria en tres dietas diferente: control, 90%(w/w) control + 10% puré de zanahoria(CP), y 90% control + 10% puré de zanahoria obtenida de zanahorias tratadas con estrés de corte(WSCP). El estrés de corte incrementó~4 veces el contenido de ácido clorogénico comparado con CP(522 mg/kg). Las ratas tratadas con las dietas WSCP y CP incrementaron el conteo de Lactobacillus en la microbiota intestinal de la segundas generación comparado con el grupo control (p ˂ 0.019). El contenido de mielina del grupo WSPC fue significativamente mayor que en los grupos control y CP en la primera generación. En la segunda generación, el contenido de mielina se incrementó a 204 mg/g en el grupo WSCP, el cual mostró mayor contenido de ARN. En general, los resultados indican que la dieta adicionada con WSCP mejora el desarrollo cerebral mediante el incremento de mielita y ARN. ARTICLE HISTORY

Valorization of Carrot Pomace: UVC Induced Accumulation of Antioxidant Phenolic Compounds

“…Previous reports have demonstrated that wounding stress application in carrots, either applied alone or in combination with UV radiation, results in the biosynthesis of antioxidant phenolic compounds [8,9]. The main phenolic compound that is accumulated is the chlorogenic acid (CHA), which has been related to the prevention and treatment of the metabolic syndrome [10], colon cancer [11,12], and the improvement in cognitive abilities and neural development [13].…”

Does Consumption of Tortured Fruit and Vegetables Improve Health, and Do They Taste Good?

“…However, none of these bioactive properties attributed to stressed broccoli and okra have been validated with in vivo models or clinical studies. Indeed, only one report has validated the nutraceutical property of a stressed crop in vivo , where it was found that carrot puree with enhanced levels of chlorogenic acid, produced from carrots treated with wounding stress, improved rat cognitive abilities and neural development . Thus, there is a need to increase the number of in vitro and in vivo studies to understand better and validate the bioactive properties of stressed fruits and vegetables.…”

Chlorogenic acid: Potential source of natural drugs for the therapeutics of fibrosis and cancer

“…1 ), due to its nomenclatural divergences [45] , [46] , [47] . CGA is usually marketed as svetol, widely obtained and distributed in herbs, foods, dicotyledonous ferns and plants species namely berry fruits, tea, apple, cocoa, coffee, citrus fruits, roasted bean, pears, carrots, wormwood, artichoke, potatoes, eggplant, betel, kiwi fruits, tobacco leaves, burdock, eucommia, coffee beans, tomatoes, honeysuckle, and grapes [48] , [49] , [50] , [51] , [52] , [53] , [54] , [55] , [56] . With regards to its health boosting attributes, CGA is also significantly applied clinically, particularly against fibrosis and cancer and serves as the main constituent in traditional herbal medicine (THM) formulations for detoxification, and heat clearance [57] , [58] , [59] , [60] , [61] .…”