Effects of centrifugal stress on cell disruption and glycerol leakage from Dunaliella salina

Xu, Yanan; Milledge, John J.; Abubakar, Abdulkadir; Swamy, Rekha A. R.; Bailey, David M.; Harvey, Patricia J.

doi:10.1515/micbi-2015-0003

Cited by 31 publications

(18 citation statements)

References 40 publications

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“…It seems that centrifugation leads more easily to the disruption of the cells and hence to higher amounts of prymnesins in the water. Xu et al [30] showed that centrifugal stress has an influence on the cell disruption and glycerol leakage of Dunaliella salina , a cell wall lacking microalgae. Detailed data for all of the time points can be found in Table S4 for the FLD-method, and in Table S5 for the HRMS-method.…”

Section: Resultsmentioning

confidence: 99%

Development of an Indirect Quantitation Method to Assess Ichthyotoxic B-Type Prymnesins from Prymnesium parvum

Svenssen

Binzer

Medić

et al. 2019

Toxins

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Harmful algal blooms of Prymnesium parvum have recurrently been associated with the killing of fish. The causative ichthyotoxic agents of this haptophyte are believed to be prymnesins, a group of supersized ladder-frame polyether compounds currently divided into three types. Here, the development of a quantitative method to assess the molar sum of prymnesins in water samples and in algal biomass is reported. The method is based on the derivatization of the primary amine group and subsequent fluorescence detection using external calibrants. The presence of prymnesins in the underivatized sample should be confirmed by liquid chromatography mass spectrometry. The method is currently only partly applicable to water samples due to the low amounts that are present. The growth and cellular toxin content of two B-type producing strains were monitored in batch cultures eventually limited by an elevated pH. The cellular toxin contents varied by a factor of ~2.5 throughout the growth cycle, with the highest amounts found in the exponential growth phase and the lowest in the stationary growth/death phases. The strain K-0081 contained ~5 times more toxin than K-0374. Further investigations showed that the majority of prymnesins were associated with the biomass (89% ± 7%). This study provides the basis for further investigations into the toxicity and production of prymnesins.

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Section: Resultsmentioning

confidence: 99%

Development of an Indirect Quantitation Method to Assess Ichthyotoxic B-Type Prymnesins from Prymnesium parvum

Svenssen

Binzer

Medić

et al. 2019

Toxins

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“…Based on our previous study, cells harvested by centrifugation at 3000 G would not be damaged and glycerol remained in cell pellets (Xu et al., 2015), therefore, 1 ml of culture samples were taken and centrifuged at 3000 G for 5 min to get the cell pellets. The pellets were then resuspended in 1 ml of distilled water and 200 μl of chloroform and vigorously vortexed to extract the glycerol.…”

Section: Methodsmentioning

confidence: 99%

The influence of photoperiod and light intensity on the growth and photosynthesis of Dunaliella salina (chlorophyta) CCAP 19/30

Ibrahim

Harvey

2016

Plant Physiology and Biochemistry

136

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The green microalga Dunaliella salina survives in a wide range of salinities via mechanisms involving glycerol synthesis and degradation and is exploited for large amounts of nutraceutical carotenoids produced under stressed conditions. In this study, D. salina CCAP 19/30 was cultured in varying photoperiods and light intensities to study the relationship of light with different growth measurement parameters, with cellular contents of glycerol, starch and carotenoids, and with photosynthesis and respiration. Results show CCAP 19/30 regulated cell volume when growing under light/dark cycles: cell volume increased in the light and decreased in the dark, and these changes corresponded to changes in cellular glycerol content. The decrease in cell volume in the dark was independent of cell division and biological clock and was regulated by the photoperiod of the light/dark cycle. When the light intensity was increased to above 1000 μmol photons m−2 s−1, cells displayed evidence of photodamage. However, these cells also maintained the maximum level of photosynthesis efficiency and respiration possible, and the growth rate increased as light intensity increased. Significantly, the intracellular glycerol content also increased, >2-fold compared to the content in light intensity of 500 μmol photons m−2 s−1, but there was no commensurate increase in the pool size of carotenoids. These data suggest that in CCAP 19/30 glycerol stabilized the photosynthetic apparatus for maximum performance in high light intensities, a role normally attributed to carotenoids.

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“…In the case of microalgae, strains of Chlorella, for example, the cell wall is a trilaminar outer layer (TLS) containing algaenan that is a very resistant and nonhydrolyzable aliphatic biopolymer, whilst other strains have a cell wall with no TLS outer layer but a greater percentage of the polysaccharide cellulose and other sugars notably glucosamine in their rigid walls [162]. The microalga D. salina does not have a true cell wall but has a cell membrane consisting of a lipid bilayer that can be readily damaged by hydrodynamic forces [163][164][165]. On the other hand, coccoliths surround their cells with an outer coating of calcium carbonate platelets [166,167].…”

Section: Cell Wall Architecturementioning

confidence: 99%

A Brief Review of Anaerobic Digestion of Algae for Bioenergy

et al. 2019

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The potential of algal biomass as a source of liquid and gaseous biofuels has been the subject of considerable research over the past few decades, with researchers strongly agreeing that algae have the potential of becoming a viable aquatic energy crop with a higher energy potential compared to that from either terrestrial biomass or municipal solid waste. However, neither microalgae nor seaweed are currently cultivated solely for energy purposes due to the high costs of harvesting, concentrating and drying. Anaerobic digestion of algal biomass could theoretically reduce costs associated with drying wet biomass before processing, but practical yields of biogas from digestion of many algae are substantially below the theoretical maximum. New processing methods are needed to reduce costs and increase the net energy balance. This review examines the biochemical and structural properties of seaweeds and of microalgal biomass that has been produced as part of the treatment of wastewater, and discusses some of the significant hurdles and recent initiatives for producing biogas from their anaerobic digestion.

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Effects of centrifugal stress on cell disruption and glycerol leakage from Dunaliella salina

Cited by 31 publications

References 40 publications

Development of an Indirect Quantitation Method to Assess Ichthyotoxic B-Type Prymnesins from Prymnesium parvum

Development of an Indirect Quantitation Method to Assess Ichthyotoxic B-Type Prymnesins from Prymnesium parvum

The influence of photoperiod and light intensity on the growth and photosynthesis of Dunaliella salina (chlorophyta) CCAP 19/30

A Brief Review of Anaerobic Digestion of Algae for Bioenergy

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