Effects of Chemotherapy and Radiotherapy on Spermatogenesis: The Role of Testicular Immunology

Qu, Ning; Itoh, Masahiro; Sakabe, Kou

doi:10.3390/ijms20040957

Cited by 63 publications

(40 citation statements)

References 98 publications

(107 reference statements)

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“…However, the presence of IL-10 indicated the return of immunoregulation in the testicular tissue. The control of the immune environment in the testicular tissue is essential to maintain immune homeostasis that is necessary for normal spermatogenesis [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Immunolocalization of IL-6 and IL-10 in the testicular tissue of testicular dysfunction rat treated with secretome

2020

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Objective: This study was carried out to find out the immunolocalization of Interleukin 6 (IL-6) and Interleukin 10 (IL-10) in the testicular tissue of testicular dysfunction rat treated with secretome from human umbilical stem cells. Materials and methods: Rats were induced with cisplatin for testicular dysfunction condition. After that, the rats were grouped into two categories and were treated with secretome at 0.2 and 0.5 ml/kg BW once every week for 4 weeks. One week later, after the secretome treatment, the rats were sacrificed for histological evaluation using the immunohistochemical method. The preparation slides were examined using a light microscope and were analyzed descriptively and quantitatively. Results: There were no IL-6 and IL-10 immunoreactivities seen in the testicular tissue after cisplatin induction. However, the immunoreactivities of IL-6 and IL-10 were detected after secretome treatment, with both dosages of 0.2 and 0.5 ml/kg BW. These immunoreactivities were detected in the spermatogonia, spermatid/luminal tissue of seminiferous tubule, spermatogenic cells, and Leydig cells. In the cell calculation, the numbers of IL-6 immunoreactive cells were higher at the lower secretome dosage. In contrast, the numbers of IL-10 immunoreactive cells were higher at the higher secretome dosage. Conclusion: IL-6 and IL-10 can be localized in the testicular tissue of testicular dysfunction rat after secretome treatment.

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Section: Discussionmentioning

confidence: 99%

Immunolocalization of IL-6 and IL-10 in the testicular tissue of testicular dysfunction rat treated with secretome

2020

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“…Radiation leading to infertility as a consequence of decrease in spermatogenesis increased apoptosis and alteration in the various hormones. The increased apoptosis is considered to be as a result of increased ROS production and DNA damage after radiotherapy (Qu, Itoh, & Sakabe, ). Exposure of adult's mice testis into γ‐radiation decreased the number of normal tubules, sperm quality and viability.…”

Section: Discussionmentioning

confidence: 99%

Hyperthermia versus busulfan: Finding the effective method in animal model of azoospermia induction

et al. 2019

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Animal models of azoospermia are very applicable when evaluating new treatment methods for research purposes. The present study aimed to compare azoospermia induction in mice using busulfan or hyperthermia. To do this, about 36 adult male mice (28–30 g) were included into three experimental groups randomly (n = 12): control, busulfan (injected by a single dose of 40 mg/kg busulfan intraperitoneally) and hyperthermia (exposure to a temperature of 43°C every other day for 5 weeks). Animals were preserved for 35 and 70 days following interventions and then were sacrificed for further evaluations. After 35 days, busulfan and hyperthermia groups revealed a significant decrease in the sperm count and weight of testis compared to the control group (p < .0001). In addition, after 70 days, sperm count and weight of testis in group busulfan showed a significant increase compared to group hyperthermia (p < .01). No significant difference was observed regarding the mortality of mice between busulfan and hyperthermia groups. In group busulfan, degenerative changes in the germinal epithelium were detected in some tubules, although in group hyperthermia, degenerative changes and complete depletion of all tubules were observed. Continuous hyperthermia is a more effective method in the induction of as animal model of azoospermia compared to the busulfan.

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“…Furthermore, the Sertoli cell blood–testis barrier is strictly regulated by cytokines such as interleukin-1α ( Il1α ), and germ cells are known to control production of this cytokine by Sertoli cells [ 31 ]. Although we did not detect BSF-induced impairment of the blood–testis barrier [ 32 ], the decreased expression of Tjp1 and the increased expression of Il1α warrant further investigation.…”

Section: Discussionmentioning

confidence: 90%

Effect of Gosha-Jinki-Gan on Levels of Specific mRNA Transcripts in Mouse Testes after Busulfan Treatment

Nagahori

Kuramasu

et al. 2020

Biomedicines

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With the increase in survival rates of cancer patients in recent years, infertility caused by anticancer treatments has become a significant concern for cancer survivors. Some studies have suggested that Sertoli cells play a key role in mediating testicular immunology in busulfan-induced aspermatogenesis. We recently demonstrated that Gosha-jinki-gan (TJ107), a traditional Japanese medicine, can completely recover injured spermatogenesis in mice 60 days after busulfan injection. In the present study, we sought to examine the levels of mRNA transcripts encoding markers of 25 Sertoli cell-specific products and 10 markers of germ cell differentiation. Our results demonstrated that only supplementation of TJ107 at day 60 after busulfan injection could significantly recover the increase in five mRNA species (Amh, Clu, Shbg, Testin, and Il1a) and the decrease in four mRNA species (Aqp8, CST9, Wnt5a, and Tjp1) in response to Busulfan (BSF) at day 120, with the increase of all examined spermatogenic markers.

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Effects of Chemotherapy and Radiotherapy on Spermatogenesis: The Role of Testicular Immunology

Cited by 63 publications

References 98 publications

Immunolocalization of IL-6 and IL-10 in the testicular tissue of testicular dysfunction rat treated with secretome

Immunolocalization of IL-6 and IL-10 in the testicular tissue of testicular dysfunction rat treated with secretome

Hyperthermia versus busulfan: Finding the effective method in animal model of azoospermia induction

Effect of Gosha-Jinki-Gan on Levels of Specific mRNA Transcripts in Mouse Testes after Busulfan Treatment

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