Effects of chlorogenic acid and caffeic acid on the quality of frozen‐thawed boar sperm

Abstract

Chlorogenic acid (CGA) and caffeic acid (CA) are potent antioxidants that are mostly found in coffee beans. This study aimed to investigate the effects of CGA and CA supplementation during semen freezing on the quality of frozen-thawed boar spermatozoa. The antioxidants CGA and CA were added to a semen extender to achieve final concentrations of 50, 100, 200 and 400 µM. Supplementation of 100 µM CGA and CA yielded a significantly higher percentage of sperm viability (increased by 8%-10%) and plasma membrane integrity (increased by 4%-6%) than the control groups without the antioxidants at 0 and 3 hr after thawing (p < 0.05). At a concentration of 100 µM, CGA and CA also yielded beneficial effects on total and progressive sperm motility. Increases of CGA and CA concentrations to more than 200 µM did not enhance any sperm quality parameters. When the sperm penetrability and oocyte development by spermatozoa frozen with CGA and CA were evaluated, CGA and CA supplementations had no positive effects on the percentages of total fertilization, monospermic fertilization, cleavage and blastocyst formation. In conclusion, the supplementation of 100 µM CGA and CA during sperm freezing improved certain sperm parameters including motility, viability and plasma membrane integrity.

Comparative Studies on Chemical Contents and Effect in Kidney-Yang Deficiency Rats of Salt-Processed Product and Wine-Processed Product of Cuscutae Semen

“…Chlorogenic acids, other active components, exhibited protective effects on sperm plasma [27, 28]. Previous studies have also shown that quercetin, astragalin, kaempferol, feruloylquinic acid, and caffeic acid have beneficial and/or protective effects on improving the reproductive function, preventing sperm damage, ameliorating sperm function, and improving testicular function [27–32]. In this study, SPP and WPP showed protective effects on reproductive, endocrine, and testicular function.…”

“…Total flavonoids, efficacious components of Cuscutae Semen, have been reported to show regulatory effects on reproductive and endocrine function [12, 26], such as regulating LH, T, and E 2 levels. Chlorogenic acids, other active components, exhibited protective effects on sperm plasma [27, 28]. Previous studies have also shown that quercetin, astragalin, kaempferol, feruloylquinic acid, and caffeic acid have beneficial and/or protective effects on improving the reproductive function, preventing sperm damage, ameliorating sperm function, and improving testicular function [27–32].…”

Protective Effect of Chlorogenic Acid on Human Sperm: In Vitro Studies and Frozen–Thawed Protocol

“…The choice of concentration for the experiments was supported by a recent observation in which the supplementation of 100 μM CGA played a beneficial effect on the quality of frozen-thawed boar spermatozoa [ 30 ]. In addition, our data indicated a positive effect of this concentration on sperm motility.…”

“…It displays different pharmacological activities such as antioxidant, anti-inflammatory, anti-microbial, hepatoprotective, anticancer, anti-lipidemic, and anti-diabetic [ 29 ]. A concentration of 100 µM CGA yielded beneficial effects on sperm motility, viability, and plasma membrane integrity in boar semen during freezing [ 30 ] and cooling at 15 °C [ 31 , 32 ]. CGA also displayed a positive effect in in vitro production of porcine embryos and a protection against DNA damage induced by OS in oocytes [ 33 ].…”

Chlorogenic Acid Ameliorates Damage Induced by Fluorene-9-Bisphenol in Porcine Sertoli Cells

“…A recent study found that CA limits apoptosis by reducing ROS production and increasing intracellular glutathione levels in HepG2 cells under oxidative stress ( Granado-Serrano et al, 2007 ). Namula et al (2018) suggested that CA supplementation improved certain sperm parameters, including viability, plasma membrane integrity, and motility, during boar sperm freezing. Nguyen et al (2019) found that CA supplementation during in vitro maturation could protect porcine oocytes from DNA damage induced by oxidative stress and improve their subsequent developmental competence after fertilization.…”