Effects of chloroplast substituted Ogura male sterile cytoplasm on the performance of cauliflower (Brassica oleracea var. botrytis L.) F1 hybrids

Dey, S. S.; Bhatia, Reeta; Sharma, Shobha; Parkash, Chander; Sureja, Amish K

doi:10.1016/j.scienta.2013.04.008

Cited by 28 publications

(21 citation statements)

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“…) and cauliflower (Dey et al. ). Effects of Ogura cybrid cytoplasm in cauliflower nuclear background are genotype specific for most of the antioxidant capacities and other nutritive traits.…”

Section: Discussionmentioning

confidence: 99%

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Alteration in important quality traits and antioxidant activities in Brassica oleracea with Ogura cybrid cytoplasm

et al. 2017

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In vegetable brassicas, cytoplasmic male sterility (CMS) system is the most preferred mechanism for hybrid seed production and Ogura cybrid cytoplasm is the only source used widely. Effects of the alien cybrid cytoplasm on important quality traits need to be understood for their effective use in breeding programme. In analysing 38 Ogura cybrid cytoplasm-based cauliflower CMS lines, it was revealed that introgression of Ogura cytoplasm resulted in significant alteration in important quality traits. Cupric reducing antioxidant activity and ferric reducing ability of plasma values were increased up to 5-10 times in different genotypes. Among 38 analysed CMS lines, concentration of plant pigments such as anthocyanin, total chlorophylls and ascorbic acid was reduced in 21, 17 and 32 genotypes, respectively. However, the concentration of total carotenoids and b-carotene was elevated in most of the CMS lines and total carotenoid was increased up to 20 times in the CMS line, Ogu13-85-2A.The results indicate the role of nuclear-cytoplasmic interaction and mitochondrial genome in determining concentration of different quality traits.

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Section: Discussionmentioning

confidence: 99%

“…The chloroplast substituted CMS lines have no significant adverse affect on agronomic performance in the nuclear background as well as the hybrids (Dey et al. ). However, influence of this chloroplast substituted lines on important quality traits such as plant pigments, ascorbic acid content and antioxidant capacity is not known.…”

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Alteration in important quality traits and antioxidant activities in Brassica oleracea with Ogura cybrid cytoplasm

et al. 2017

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“…This undesirable effect is due to the incompatibility between chloroplast and nucleus, and the problem could be overcome by chloroplast substitution, which involved somatic hybridization and repeated backcrossing to cabbage (Dey et al 2013). With similar approaches being tried, more detailed understanding of the mechanism by which leaf chlorosis is induced can accelerate breeding efforts for Chinese cabbage.…”

Section: Discussionmentioning

confidence: 99%

Chlorosis of Ogura-CMS Brassica rapa is due to down-regulation of genes for chloroplast proteins

Jeong

Song

et al. 2017

J Plant Biotechnol

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract Cytoplasmic male sterility (CMS) is a maternally inherited trait leading to loss of the ability to produce fertile pollen and is extensively used in hybrid crop breeding. Ogura-CMS was originally generated by insertion of orf138 upstream of atp8 in the radish mitochondrial genome and transferred to Brassica crops for hybrid breeding. Gene expression changes by dysfunctional mitochondria in Ogura-CMS result in pollen developmental defects, but little is known about gene expression patterns in vegetative tissue. To examine the interaction between nuclear and organellar regulation of gene expression, microarray and subsequent gene expression experiments were conducted with leaves of F 1 hybrid Chinese cabbage derived from selfincompatible (SI) or Ogura-CMS parents (Brassica rapa ssp. pekinensis). Out of 24,000 genes deposited on a KBGP24K microarray, 66 genes were up-regulated and 26 genes were down-regulated by over 2.5 fold in the CMS leaves. Up-regulated genes included stress-response genes and mitochondrial protein genes, while genes for ascorbic acid biosynthesis and thylakoid proteins were down-regulated. Most of the major component genes for light reactions of photosynthesis were highly expressed in leaves of both SI and CMS plants, but most of the corresponding proteins were found to be greatly reduced in leaves of CMS plants, indicating posttranscriptional regulation. Reduction in thylakoid proteins and chlorophylls led to reduction in photosynthetic efficiency and chlorosis of Ogura-CMS at low temperatures. This research provides a foundation for studying chloroplast function regulated by mitochondrial signal and for using organelle genome introgression in molecular breeding.

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“…The most major CMS in B. oleracea is referred as improved Ogura CMS discovered from Japanese radish ( Raphanus sativus L.) (Bannerot, Loulidard, Cauderon, & Tempe, ; Dey, Bhatia, Sharma, Parkash, & Sureja, & Ogura, ). It is also used worldwide in F 1 seed production of B. napus , B. juncea and R. sativus (Dey, Sharma, Bhatia, Kumar, & Prakash, ; Dey, Sharma, Bhatia, Parkash, & Barwal, ; Kagami, Abamatsu, & Shiga, ).…”

Section: Introductionmentioning

confidence: 99%

“…If new CMS system had no advantage compared to current CMS system, most major CMS systems would continue to be used in Brassicaceae crops. Therefore, the improved Ogura CMS system with no adverse effect has been relied on as a regular CMS system for the F 1 seed production in B. oleracea (Dey et al., ).…”

Section: Introductionmentioning

confidence: 99%

Production of a desirable Brassica oleraceaCMS line using an alloplasmic B. rapaCMS line carrying Diplotaxis erucoides cytoplasm as a bridge plant

et al. 2018

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In Brassica oleracea, production of F1 hybrid seeds mainly makes use of the improved Ogura cytoplasmic male sterile (CMS) line. However, reliance on one particular line is a risk, and it would be advantageous to develop other CMS lines. In this study, we transferred Diplotaxis erucoides cytoplasm to B. oleracea cultivars using an alloplasmic B. rapaCMS line as a bridge plant to avoid incompatibility between donor and recipient plants. The new B. oleraceaCMS lines, which were derived by four generations of backcrossing, had small rudimentary anthers with no pollen grain and showed complete male sterility. There was no functional defect in other floral organs, and the ability to receive normal pollen did not appear to be impaired. Moreover, the B. oleraceaCMS lines carrying D. erucoides cytoplasm had larger leaf areas and a normal plastochron. As a consequence, the B. oleraceaCMS lines carrying D. erucoides cytoplasm have the potential to be valuable alternatives for use in commercial B. oleracea hybrid seed production.

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Effects of chloroplast substituted Ogura male sterile cytoplasm on the performance of cauliflower (Brassica oleracea var. botrytis L.) F1 hybrids

Cited by 28 publications

References 14 publications

Alteration in important quality traits and antioxidant activities in Brassica oleracea with Ogura cybrid cytoplasm

Alteration in important quality traits and antioxidant activities in Brassica oleracea with Ogura cybrid cytoplasm

Chlorosis of Ogura-CMS Brassica rapa is due to down-regulation of genes for chloroplast proteins

Production of a desirable Brassica oleraceaCMS line using an alloplasmic B. rapaCMS line carrying Diplotaxis erucoides cytoplasm as a bridge plant

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