Effects of chlorpyrifos on life cycle parameters, cytochrome P450S expression, and antioxidant systems in the monogonont rotifer <i>Brachionus koreanus</i>

Kim, Ryeo-Ok; Kim, Bomi; Jeong, Chang‐Bum; Lee, Jae‐Seong; Rhee, Jae-Sung

doi:10.1002/etc.3288

Cited by 24 publications

(7 citation statements)

References 74 publications

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“…buffer volume, employed basic instruments) [26–29]. GSH concentration was determined by an enzymatic method with the Glutathione Assay Kit (Catalog No.…”

Section: Methodsmentioning

confidence: 99%

Effects of Antifouling Biocides on Molecular and Biochemical Defense System in the Gill of the Pacific Oyster Crassostrea gigas

Park

Kim

et al. 2016

PLoS ONE

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Antifouling biocides such as organotin compounds and their alternatives are potent toxicants in marine ecosystems. In this study, we employed several molecular and biochemical response systems of the Pacific oyster Crassostrea gigas to understand a potential mode of action of antifouling biocides (i.e. tributyltin (TBT), diuron and irgarol) after exposure to different concentrations (0.01, 0.1, and 1 μg L-1) for 96 h. As a result, all the three antifouling biocides strongly induced the antioxidant defense system. TBT reduced both enzymatic activity and mRNA expression of Na+/K+-ATPase and acetylcholinesterase (AChE). Lower levels of both Na+/K+-ATPase activity and AChE mRNA expression were observed in the diuron-exposed oysters compared to the control, while the irgarol treatment reduced only the transcriptional expression of AChE gene. We also analyzed transcript profile of heat shock protein (Hsp) superfamily in same experimental conditions. All antifouling biocides tested in this study significantly modulated mRNA expression of Hsp superfamily with strong induction of Hsp70 family. Taken together, overall results indicate that representative organotin TBT and alternatives have potential hazardous effects on the gill of C. gigas within relatively short time period. Our results also suggest that analyzing a series of molecular and biochemical parameters can be a way of understanding and uncovering the mode of action of emerging antifouling biocides. In particular, it was revealed that Pacific oysters have different sensitivities depend on the antifouling biocides.

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“…buffer volume, employed basic instruments) [26–29]. GSH concentration was determined by an enzymatic method with the Glutathione Assay Kit (Catalog No.…”

Section: Methodsmentioning

confidence: 99%

Effects of Antifouling Biocides on Molecular and Biochemical Defense System in the Gill of the Pacific Oyster Crassostrea gigas

Park

Kim

et al. 2016

PLoS ONE

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“…The continuous use of pesticides even in normal recommended doses may cause deleterious effect on the physiological functions [ 5 - 8 ] that may range from lower immunity of flock [ 9 , 10 ] to decrease production performances [ 11 ]. The lower immune competence in animals and birds due to environmental pollutant may lead to increase susceptibility [ 12 , 13 ], occurrence of reinfection, epidemics of disease [ 5 , 14 , 15 ], and vaccine failures [ 16 ] causing serious economic losses and thereby hampering the purpose of raising animals and birds. Hematological, biochemical, and pathological effect of chronic exposure to insecticides were also evaluated in indigenous chickens [ 17 - 20 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of endosulfan on immunological competence of layer birds

et al. 2016

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Aim:The present study was aimed to investigate the immunological competence of endosulfan insecticide after limited oral administration in White Leghorn layer chickens.Materials and Methods:A total of 20 White Leghorn birds were given endosulfan in drinking water at 30 ppm/bird/day (no observable effect level dose) for a period of 3-months. Immune competence status of layer birds and chicks hatched from endosulfan offered birds were estimated at 15-day interval in layer birds and at monthly interval in chicks using immunological, biochemical parameters, and teratological estimates.Results:There was a significant decrease in levels of total leukocytes count, absolute lymphocyte count, absolute heterophil count, total serum protein, serum albumin, serum globulin, and serum gamma globulin in the birds fed with endosulfan as compared to control. Similarly, immune competence tests such as lymphocyte stimulation test, oxidative burst assay, and enzyme-linked immunosorbent assay tests indicated lower immunity in birds treated with endosulfan as compared to control. Subsequently, chicks produced from endosulfan-treated birds were also examined for immune competence, but no significant difference was observed between chicks of both the groups.Conclusion:The exposure to endosulfan in limited oral dosage was able to exhibit hemo-biochemical and other changes that could be correlated with changes in the immunological profile of layer chickens suggesting cautious usage of endosulfan insecticide in poultry sheds.

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“…In particular, the transcription of isoforms ( CYP3042A1 and CYP3043A1 ) in CYP clan 2 was significantly induced ( P < 0.01) . In organophosphorus insecticide-exposed rotifer B. koreanus , Bk-CYP3046A1 and several isoforms in CYP Clans 2, 3, and 46 were sensitively up-regulated . However, in triphenyltin chloride-exposure, the Bk-CYP3045C1 gene was highly expressed (up to 62.6 times higher than in the control), as shown in other environmental toxicant (bisphenol A and drugs)-exposed rotifer B. koreanus (data not shown).…”

Section: Discussionmentioning

confidence: 62%

Adverse Effects, Expression of the Bk-CYP3045C1 Gene, and Activation of the ERK Signaling Pathway in the Water Accommodated Fraction-Exposed Rotifer

Won

Kim

Kang

et al. 2016

Environ. Sci. Technol.

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To examine the deleterious effects of the water accommodated fraction (WAF) of crude oil, the growth curve, fecundity, and lifespan of the monogonont rotifer (Brachionus koreanus) were measured for 24 h in response to three different doses (0.2×, 0.4×, and 0.8×) of WAFs. A higher dose of WAFs significantly reduced the fecundity and lifespan. A rotifer 32K microarray chip showed that the Bk-CYP3045C1 gene had the highest expression. Of the 25 entire CYP genes, the Bk-CYP3045C1 gene showed a significant expression for different doses and times in response to WAFs and chemical components of WAFs (naphthalene and phenanthrene); also, glutathione S-transferase genes, ABC transporter, and other genes showed dose responses upon exposure to 80% WAF over time. Different doses of WAFs increased the oxidative stress with an induction of reactive oxygen species (ROS) and a depletion of glutathione (GSH). Exposure to WAFs did not show toxic effects on survivability in B. koreanus; however, toxicity to WAFs was shown when piperonyl butoxide, a potent inhibitor of cytochrome P450 (CYP) enzymes, was added. This toxicity was dose-dependent. After WAFs exposure, p-ERK was activated over time in response to WAFs, which suggests that WAFs can be activated by the p-ERK signaling pathway.

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Effects of chlorpyrifos on life cycle parameters, cytochrome P450S expression, and antioxidant systems in the monogonont rotifer Brachionus koreanus

Cited by 24 publications

References 74 publications

Effects of Antifouling Biocides on Molecular and Biochemical Defense System in the Gill of the Pacific Oyster Crassostrea gigas

Effects of Antifouling Biocides on Molecular and Biochemical Defense System in the Gill of the Pacific Oyster Crassostrea gigas

Effect of endosulfan on immunological competence of layer birds

Adverse Effects, Expression of the Bk-CYP3045C1 Gene, and Activation of the ERK Signaling Pathway in the Water Accommodated Fraction-Exposed Rotifer

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