Effects of chlortetracycline of honey bee worker larvae reared in vitro

Peng, Ying; Mussen, Eric C.; Fong, April; Montague, Mary Ann; Tyler, Tim

doi:10.1016/0022-2011(92)90085-i

Cited by 110 publications

(88 citation statements)

References 27 publications

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“…Substantial progress has been made with bioassays that can be used to rear immature honey bees in the laboratory over the past few decades (Aupinel et al, 2005;Crailsheim et al, 2013;Huang, 2009;Peng, Mussen, Fong, Montague, & Tyler, 1992;Rembold & Lackner, 1981;Vandenberg & Shimanuki, 1987). The in vitro methodology for workers was initially used to investigate queen/worker differentiation, nutrition, and pathogen impacts on immature bees (Rembold & Lackner, 1981;Vandenberg & Shimanuki, 1987).…”

Section: Introductionmentioning

confidence: 99%

“…Vandenberg and Shimanuki (1987) further improved the in vitro methodology by optimizing diet composition and the amount of provisioned diet, both of which yielded higher rates of adult emergence and less queen intermediates. Peng et al (1992) began utilizing the methodology for determining the toxicity of pesticides to developing honey bees. Other rearing strategies have been developed (Hendriksma, Härtel, & Steffan-Dewenter, 2011b;Huang, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…The advancements in the in vitro rearing protocol have given government, industry, and academia a new tool for accessing the risks pesticides and other chemicals pose to immature honey bee development and survival (Aupinel et al, 2005(Aupinel et al, , 2007(Aupinel et al, , 2009Crailsheim et al, 2013;Hendriksma, Härtel, & Steffan-Dewenter, 2011b;Huang, 2009;Peng et al, 1992;Zhu, Schmehl, Mullin, & Frazier, 2014). Honey bee in vitro rearing protocols have allowed researchers to standardize environmental conditions (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Protocol for the in vitro rearing of honey bee (Apis mellifera L.) workers

Schmehl

Tomé

Mortensen

et al. 2016

Journal of Apicultural Research

107

145

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The in vitro rearing of worker honey bees (Apis mellifera L.) has become an increasingly important method in honey bee research in general, and in pesticide risk assessment specifically. Authorities from the European Organization for Economic Co-operation and Development and the United States Environmental Protection Agency are requesting data on pesticide impacts on immature bee survivorship prior to registering new crop protection products. Those using the current in vitro rearing protocols have had variable success with immature bee survival and protocol repeatability. Here, we present an improved method for the in vitro rearing of worker honey bees from larvae to adult emergence. We have achieved consistently high survival (>95%) in our control and solvent-control rearing trials. Changes in the proportion of diet components, royal jelly source, maintenance of the developing bee, and rearing environment are the main contributors for our high rearing success and are discussed herein. Our in vitro rearing protocol can be implemented as the standard protocol to determine the impact of pesticides on immature bees because of the protocol's high control survivability, ease in end point determination, and high overall repeatability. Protocolo para la cría in vitro de obreras de Apis melliferaLa cría in vitro de las abejas obreras (Apis mellifera L.) se ha convertido en un método cada vez más importante en la investigació n de la abeja de la miel en general, y específicamente, en la evaluació n del riesgo de los plaguicidas. Las autoridades de la Organizació n Europea para la Cooperació n y el Desarrollo, y la Agencia de Protecció n Ambiental de los Estados Unidos están solicitando datos sobre los efectos de los plaguicidas en la supervivencia de abejas inmaduras antes de registrar nuevos productos para la protecció n de los cultivos. Aquellos que utilizan actualmente los protocolos de la cría in vitro han tenido un éxito variable en la supervivencia de abejas inmaduras y la repetitividad del protocolo. Aquí presentamos un método mejorado para la cría in vitro de las abejas obreras a partir de larvas para la eclosió n de los adultos. Hemos logrado consistentemente una alta supervivencia (>95%) en nuestro control y en los ensayos de cría con el disolvente del control. Los cambios en la proporció n de los componentes de la dieta, la fuente de jalea real, el mantenimiento de las abejas en desarrollo, y el ambiente de cría son los principales constituyentes para nuestro alto éxito de cría y son discutidos en este trabajo. Nuestro protocolo de cría in vitro puede ser implementado como el protocolo estándar para determinar el impacto de los plaguicidas en las abejas inmaduras debido a la alta capacidad de supervivencia del control, la facilidad en la determinació n del punto final, y la alta capacidad de repetitividad.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Protocol for the in vitro rearing of honey bee (Apis mellifera L.) workers

Schmehl

Tomé

Mortensen

et al. 2016

Journal of Apicultural Research

107

145

View full text Add to dashboard Cite

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“…Some antibiotics, such as oxytetracycline (OTC) hydrochloride and sulfathiazole, are commonly used for the treatment of infected colonies, but in most European countries, the use of these antibiotics is banned. Common problems associated with antibiotic use include the ineffectiveness of these agents against the infectious spores, adverse effects on the vitality of the brood and the longevity of the bees, [6] and an increased rate of resistance of various P. larvae strains. Furthermore, antibiotic residues present in honey represent a major human health hazard.…”

Section: Introductionmentioning

confidence: 99%

In vitroevaluation of the effects of some plant essential oils onPaenibacillus larvae, the causative agent of American foulbrood

Ansari

Al-Ghamdi

Usmani

et al. 2015

Biotechnology & Biotechnological Equipment

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Paenibacillus larvae is one of the major bacterial pathogens of honey bee broods and the causative agent of American foulbrood disease. The factors responsible for the pathogenesis of American foulbrood disease are still not fully understood, and the increasing resistance of P. larvae to commonly used antibiotics necessitates a search for new agents to control this disease. The in vitro antibacterial activities of 28 plant essential oils against P. larvae ATCC 9545 were evaluated. Out of the 28 plant essential oils tested, 20 were found to be effective in killing P. larvae. Based on their minimum bactericidal concentration (MBC) values, the effective oils were grouped into three categories: highly effective, moderately effective and minimally effective. Jamaica pepper oil, mountain pepper oil, ajwain oil, corn mint oil, spearmint oil, star anise oil, nutmeg oil and camphor oil were highly effective, with MBC values between 162.0 and 375.0 mg/mL. Jamaica pepper oil was the most effective essential oil, with an MBC value of 162.0 mg/mL. The results of the time-response effect assays showed that no viable P. larvae cells were observed after 24 h of treatment with Jamaica pepper oil (162.0 mg/mL), 36 h of treatment with mountain pepper oil (186.0 mg/mL), 48 h of treatment with ajwain oil (224.8 mg/mL) or 48 h of treatment with oxytetracycline (5.89 mg/mL). The tested essential oils exhibited significant antimicrobial activities against P. larvae, and they may contain compounds that could play an important role in the treatment or prevention of American foulbrood disease.

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“…In most cases, methods based on opening the hive during collection (eg, Schnetter, 1934;Rembold et al, 1992) will interrupt the egg-laying pattern. We have run such a preparative system for 3 years, partly by using available knowledge (Jay, 1964;Praagh, 1972Praagh, , 1975Doull, 1976;Rembold and Lackner, 1981;Peng et al, 1992), and partly by developing new protocols (Omholt et al, 1994 (fig 1 c, d). Le (fig 1 f).…”

Section: Introductionmentioning

confidence: 99%

A laboratory hive for frequent collection of honeybee eggs

Omholt¹,

Hagen²,

Elmholdt³

et al. 1995

Apidologie

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Summary — We describe a hive design that allows frequent collection of eggs of a defined age with only slight disturbance of the worker bees or the queen. The hive may be operated in the bee yard or in a flight room, and has proven to provide a steady supply of eggs throughout the year. The eggs are placed on small wax-coated plastic cell bases serving as removable bottoms of normal worker cells and are collected without opening the hive. This also makes the eggs more accessible for laboratory manipulation compared to eggs deposited in ordinary frames.

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Effects of chlortetracycline of honey bee worker larvae reared in vitro

Cited by 110 publications

References 27 publications

Protocol for the in vitro rearing of honey bee (Apis mellifera L.) workers

Protocol for the in vitro rearing of honey bee (Apis mellifera L.) workers

In vitroevaluation of the effects of some plant essential oils onPaenibacillus larvae, the causative agent of American foulbrood

A laboratory hive for frequent collection of honeybee eggs

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