Effects of chondroitin sulfate and interleukin-1β on human articular chondrocytes cultivated in clusters

Bassleer, C.; Combal, Jean-Philippe; Bougaret, S.; Malaise, Michel

doi:10.1053/joca.1998.0112

Cited by 100 publications

(84 citation statements)

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“…21,22) In addition, CS has been reported not to bind to IL-1b. 23) Both CS-SC and CS-PC effectively interfered with the IL1b-enhanced gene expression of ADAMTS-4 and -5 in human articular chondrocytes and synovial fibroblasts. Messenger RNA levels of ADAMTS-4 and -5 well correlate with their enzymatic activities, 24,25) therefore CS-SC and CS-PC are likely to attenuate the aggrecanase-mediated destruction of articular cartilage in OA.…”

Section: Csmentioning

confidence: 93%

Anti-arthritic Action Mechanisms of Natural Chondroitin Sulfate in Human Articular Chondrocytes and Synovial Fibroblasts

Imada

Oka

Kawasaki

et al. 2010

Biological & Pharmaceutical Bulletin

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Section: Csmentioning

confidence: 93%

Anti-arthritic Action Mechanisms of Natural Chondroitin Sulfate in Human Articular Chondrocytes and Synovial Fibroblasts

Imada

Oka

Kawasaki

et al. 2010

Biological & Pharmaceutical Bulletin

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“…The decrease in pain and swelling may be explained by an anti-inflammatory effect of chondroitin sulfate, probably through diverse mechanisms, such as diminishing the expression of phospholipase A2 (PLA2) [13], of cyclooxygenase-2, and the concentrations of prostaglandin E 2 , of nitric oxide [14,15], and of reactive oxygen species [14][15][16][17].…”

mentioning

confidence: 99%

Chondroitin Sulfate Inhibits the Nuclear Translocation of Nuclear Factor‐κB in Interleukin‐1β‐Stimulated Chondrocytes

Jomphe

Gabriac

Hale

et al. 2007

Basic Clin Pharma Tox

108

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Chondroitin sulfate is referred as a symptomatic slow-acting drug for osteoarthritis because it improves articular function, and reduces joint swelling and effusion. In addition, chondroitin sulfate prevents joint space narrowing of the knee. We hypothesized that the anti-inflammatory effect of chondroitin sulfate is associated to a decrease in the activation of mitogen-activated protein kinases (MAPK) and of the transcription factors nuclear factor-κ B (NF-κ B) and activator protein-1 (AP-1). Cultured rabbit chondrocytes were stimulated with interleukin-1 β (IL-1 β ) in presence of chondroitin sulfate. Nuclear translocation of NF-κ B and AP-1, and nitrite concentrations (as an index for nitric oxide) was assessed 48 hr later. The effect of chondroitin sulfate on IL-1 β activation of extracellular signal-regulated kinase 1/2 (Erk1/2) and p38MAPK was documented by immunoblot. The effect of chondroitin sulfate on sodium nitroprusside-induced apoptosis was evaluated with the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling assay. Chondroitin sulfate reduced IL-1 β -induced NF-κ B nuclear translocation, but not AP-1 translocation, it decreased IL-1 β -induced phosphorylation of Erk1/2 and abrogated p38MAPK phosphorylation, but did not prevent IL-1 β -induced increase in nitrite. Finally, chondroitin sulfate decreased nitroprusside-induced apoptosis of the chondrocytes. These results suggest that some of the biological activities of chondroitin sulfate may be associated to the reduction in Erk1/2 and p38MAPK phosphorylation and nuclear transactivation of NF-κ B.

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“…CS increased the synthesis of hyaluronate in synovial cells [Verbruggen and Veys, 1977]. Since then, it was also demonstrated to increase type II collagen and proteoglycan (PG) synthesis in human chondrocytes [Wang et al 2002;Bassleer et al 1998] and GAG in bovine chondrocytes [Legendre et al 2008]. This effect can also be secondary to matrix metalloproteinase (MMP) downregulation [Wang et al 2002].…”

Section: Anticatabolic and Anabolic Effectsmentioning

confidence: 99%

Chondroitin sulfate in the treatment of osteoarthritis: from in vitro studies to clinical recommendations

Henrotin

Mathy

Sanchez

et al. 2010

Therapeutic Advances in Musculoskeletal

150

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Chondroitin sulfate (CS) is recommended as a therapeutic intervention in the multimodal approach of osteoarthritis (OA) management. CS has been studied extensively to describe its pharmacology (pharmacokinetic, in vitro and in vivo effects) and its clinical efficacy. Various results have been reported depending on the system of evaluation (model, dosage and duration) and the source of CS (origin and quality). The purpose of this review was to gather most of the available information about CS and to discuss its potency in OA management.

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Effects of chondroitin sulfate and interleukin-1β on human articular chondrocytes cultivated in clusters

Abstract: These in vitro studies suggest that ACS is able to increase matrix component production by human chondrocytes and to inhibit the negative effects of IL-1 beta.

Cited by 100 publications

References 0 publications

Anti-arthritic Action Mechanisms of Natural Chondroitin Sulfate in Human Articular Chondrocytes and Synovial Fibroblasts

Anti-arthritic Action Mechanisms of Natural Chondroitin Sulfate in Human Articular Chondrocytes and Synovial Fibroblasts

Chondroitin Sulfate Inhibits the Nuclear Translocation of Nuclear Factor‐κB in Interleukin‐1β‐Stimulated Chondrocytes

Chondroitin sulfate in the treatment of osteoarthritis: from in vitro studies to clinical recommendations

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