Effects of chronic stimulation with different impulse patterns on the expression of myosin isoforms in rat myotube cultures

Naumann, Karin; Pette, Dirk

doi:10.1046/j.1432-0436.1994.5530203.x

Cited by 87 publications

(68 citation statements)

References 43 publications

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“…Slow muscle fibers were identified with anti-S58 (Miller et al, 1985) (Fig. 6 A, AЈ, D , DЈ,G,GЈ), whereas fast muscle fibers were identified with anti-A4.74 (data not shown) (Jergović et al, 2001) or mouse anti-skeletal myosin (Naumann and Pette, 1994) (Fig. 6 B, BЈ, E, EЈ, H, HЈ).…”

Section: Emg Recordings Independently Verified Individual Mus As Demmentioning

confidence: 93%

Transplanted Mouse Embryonic Stem-Cell-Derived Motoneurons Form Functional Motor Units and Reduce Muscle Atrophy

Yohn¹,

Miles²,

Rafuse³

et al. 2008

J. Neurosci.

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Prolonged muscle denervation resulting from motor neuron (MN) damage leads to atrophy and degeneration of neuromuscular junctions (NMJs), which can impart irreversible damage. In this study, we ask whether transplanted embryonic stem (ES) cells differentiated into MNs can form functional synapses with host muscle, and if so what effects do they have on the muscle. After transplantation into transected tibial nerves of adult mice, ES-cell-derived MNs formed functional synapses with denervated host muscle, which resulted in the ability to produce average tetanic forces of 44% of nonlesioned controls. ES-cell-derived motor units (MUs) had mean force values and ranges similar to control muscles. The number of type I fibers and fatigue resistance of the MUs were increased, and denervationassociated muscle atrophy was significantly reduced. These results demonstrate the capacity for ES-cell-derived MNs not only to incorporate into the adult host tissue, but also to exert changes in the target tissue. By providing the signals normally active during embryonic development and placing the cells in an environment with their target tissue, ES cells differentiate into MNs that give rise to functional MU output which resembles the MU output of endogenous MNs. This suggests that these signals combined with those present in the graft environment, lead to the activation of a program intended to produce a normal range of MU forces.

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Section: Emg Recordings Independently Verified Individual Mus As Demmentioning

confidence: 93%

Transplanted Mouse Embryonic Stem-Cell-Derived Motoneurons Form Functional Motor Units and Reduce Muscle Atrophy

Yohn¹,

Miles²,

Rafuse³

et al. 2008

J. Neurosci.

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“…Primary cultures of muscle myoblasts can be obtained from neonatal rat or mouse skeletal muscles (92,(712)(713)(714)(715). Depending on the composition of the culture medium, myoblasts can be maintained in a proliferative state, or they can be induced to fuse and form spontaneously contracting myotubes.…”

Section: And Recommendation 65mentioning

confidence: 99%

“…Depending on the composition of the culture medium, myoblasts can be maintained in a proliferative state, or they can be induced to fuse and form spontaneously contracting myotubes. The interaction of thyroid hormones and contractile activity on myotube properties can be studied by inhibiting contractions (715) or by direct electrical stimulation of the myotubes in culture (712). Although primary cultures provide a versatile model to study different aspects of muscle gene regulation, it should be noted that myotubes do not fully develop adult phenotypic characteristics.…”

Section: And Recommendation 65mentioning

confidence: 99%

American Thyroid Association Guide to Investigating Thyroid Hormone Economy and Action in Rodent and Cell Models

Bianco

Anderson

Forrest

et al. 2014

Thyroid

175

106

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“…In vitro studies have attempted to ascertain the effects of exercise and training by applying different stimuli. The first study (18) removed cells from rat hindlimb and soleus muscle and applied electrical stimuli via a culture solution to measure expression of myosin heavy chain (MyHC) isoforms. In the second study (11), osteoblasts were compressed using glass boards to ascertain morphological and bio-cubation, proliferation and properties remain relatively stable (29).…”

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confidence: 99%

Effects of stretching stimulation with different rates on the expression of MyHC mRNA in mouse cultured myoblasts

et al. 2007

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In vivo studies have shown that changes in the characteristics of skeletal muscle fiber are determined by type of exercise or training. These earlier studies on mechanical stimulation, however, have all employed stimulation applied at a constant intensity, and no studies appear to have investigated change with variation of intensity of stimulation. In this study, we investigated the characteristics and differentiation of myoblasts stretched at different rates. Myoblasts were stimulated at 3 different rates, and the numbers of cells and nuclei on days 1, 3, and 5 were compared. The myosin heavy chain (MyHC) mRNA expression level was also compared. We investigated expression of MyHC-perinatal to determine speed of differentiation of myoblasts, and expression of MyHC2b, 2d, and 2a to ascertain muscle cell characteristics. Counting cells and nuclei of myoblasts revealed clear promotion of differentiation with stretching. With rapid stretching, expression of MyHC-perinatal was high at first, but then showed a decrease. In terms of effect on muscle fiber characteristics, MyHC-2b, MyHC-2d, and MyHC-2a were high with rapid, medium, and slow stretching, respectively. This indicated that myoblast differentiation was promoted regardless of difference in stretching speed, with the myoblasts acquiring the muscle-fiber characteristics appropriate to each rate of stretching.Exercise and training are known to bring about changes in the characteristics of skeletal muscle fibers (26,27). Numerous studies of the human (2,3,4,12,13,17,20,24) have shown that skeletal muscle fiber characteristics change depending on the mode of exercise. Anaerobic exercise facilitates increases in fast-twitch muscle, while aerobic exercise facilitates increases in slow-twitch muscle. Studies using rats have reported that long-term low-intensity exercise increases slow-twitch muscle and decreases fast-twitch muscle (9,15). In this manner, in vivo studies in the rat and the human have shown that exercise changes muscle fiber characteristics. From the perspective of stress, severing a nerve controlling skeletal muscle not only decreases muscle weight, but also changes muscle fiber characteristics (7,14). Studies in which muscle blood flow was regulated using devices such as a tourniquet have shown that even low-intensity training caused marked muscle hypertrophy and increased muscle force (25). However, few in vitro studies have been conducted to support and clarify these findings. In vitro studies have attempted to ascertain the effects of exercise and training by applying different stimuli. The first study (18) removed cells from rat hindlimb and soleus muscle and applied electrical stimuli via a culture solution to measure expression of myosin heavy chain (MyHC) isoforms. In the second study (11), osteoblasts were compressed using glass boards to ascertain morphological and bio-

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Effects of chronic stimulation with different impulse patterns on the expression of myosin isoforms in rat myotube cultures

Cited by 87 publications

References 43 publications

Transplanted Mouse Embryonic Stem-Cell-Derived Motoneurons Form Functional Motor Units and Reduce Muscle Atrophy

Transplanted Mouse Embryonic Stem-Cell-Derived Motoneurons Form Functional Motor Units and Reduce Muscle Atrophy

American Thyroid Association Guide to Investigating Thyroid Hormone Economy and Action in Rodent and Cell Models

Effects of stretching stimulation with different rates on the expression of MyHC mRNA in mouse cultured myoblasts

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