Effects of cigarette smoke extract on bronchial epithelial cells stimulated with Cryptococcus neoformans

Pereira, Aline Beatriz Mahler; Oliveira, Jhony Robison de; Souza, Ariani Impieri de; Andrade-Silva, Leonardo; Silva, Marcos; Silva, Paulo Roberto da; Silva‐Vergara, Mario León; Rogério, Alexandre de Paula

doi:10.1007/s00430-021-00715-4

Cited by 4 publications

(5 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, C. neoformans adheres to and can be internalized by A549 and BEAS-2B cells [ 115 , 116 ]. Cigarette smoke extract increases adhesion and stimulates fungal growth but reduces the internalization of Cryptococcus in BEAS-2B cells, enabling growth in the airways [ 117 ]. While Aspergillus can be internalized, the epithelial cell often does not kill the fungus.…”

Section: The Human Airway Epithelium Is a Key Player In The Host-fung...mentioning

confidence: 99%

“…Secretome analyses in BEAS-2B cells demonstrated that Aspergillus triggered thioredoxin activation, lysosomal degranulation, and cathepsin, suggesting immune defense systems within these cells [ 121 ]. Further studies identified the production of critical inflammatory and antimicrobial peptides following infection by fungal pathogens, including IL-6, IL-8, granulocyte-macrophage colony-stimulating factor (GM-CSF), human β-defensin (HBD)2, HBD9, chemokine ligand (CXCL)1, monocyte chemoattractant protein-1 (MCP-1), and TNFα, with IL-6 and IL-8 being the most widely studied [ 103 , 110 , 116 , 117 , 122 ].…”

Section: The Human Airway Epithelium Is a Key Player In The Host-fung...mentioning

confidence: 99%

“…In the setting of Aspergillus infection, Dectin-1 and CR3 mediated release of IL-8 and MCP-1 through induction of NFκB [ 127 ]. The pro-inflammatory signal transducer and activator of transcription 3 (STAT3)-mediated IL-6 stimulation and anti-inflammatory response (e.g., chemokines C-C motif chemokine ligand 2 [CCL2]/extracellular signal regulated kinase ½ [ERK1/2] and IL-10) have been demonstrated in cryptococcal infections in vitro in combination with either cigarette smoke or Dermatophagoides pteronyssinus (a trigger for allergic asthma) [ 117 , 128 ]. While many of these studies provide a window into the human lung, they primarily utilize immortalized and oncologic cell lines and need to be validated in primary ALI systems.…”

Section: The Human Airway Epithelium Is a Key Player In The Host-fung...mentioning

confidence: 99%

See 2 more Smart Citations

Human Airway Epithelium Responses to Invasive Fungal Infections: A Critical Partner in Innate Immunity

Crossen

Ward

Reedy

et al. 2022

JoF

View full text Add to dashboard Cite

The lung epithelial lining serves as the primary barrier to inhaled environmental toxins, allergens, and invading pathogens. Pulmonary fungal infections are devastating and carry high mortality rates, particularly in those with compromised immune systems. While opportunistic fungi infect primarily immunocompromised individuals, endemic fungi cause disease in immune competent and compromised individuals. Unfortunately, in the case of inhaled fungal pathogens, the airway epithelial host response is vastly understudied. Furthering our lack of understanding, very few studies utilize primary human models displaying pseudostratified layers of various epithelial cell types at air-liquid interface. In this review, we focus on the diversity of the human airway epithelium and discuss the advantages and disadvantages of oncological cell lines, immortalized epithelial cells, and primary epithelial cell models. Additionally, the responses by human respiratory epithelial cells to invading fungal pathogens will be explored. Future investigations leveraging current human in vitro model systems will enable identification of the critical pathways that will inform the development of novel vaccines and therapeutics for pulmonary fungal infections.

show abstract

Section: The Human Airway Epithelium Is a Key Player In The Host-fung...mentioning

confidence: 99%

Section: The Human Airway Epithelium Is a Key Player In The Host-fung...mentioning

confidence: 99%

Section: The Human Airway Epithelium Is a Key Player In The Host-fung...mentioning

confidence: 99%

See 1 more Smart Citation

Human Airway Epithelium Responses to Invasive Fungal Infections: A Critical Partner in Innate Immunity

Crossen

Ward

Reedy

et al. 2022

JoF

View full text Add to dashboard Cite

show abstract

“…Alveolar macrophages from mice infected with C. neoformans have increased NF-κB phosphorylation [45]. However, the NF-κB activity in lung epithelial cells could be entirely dispensable for the development of airway in ammation in response to to C. neoformans [41]. Earlier results from our group demonstrated activation of NF-κB by C. neoformans in BEAS-2B cells [13,29].…”

Section: Discussionmentioning

confidence: 81%

“…However the modulation of IL-8 in the hyperglycemia depend on type of cells once its production did not change in smooth muscle cells, but increase in human umbilical venous endothelial cells (HUVEC) and aortic endothelial cells [40]. Earlier results from our group demonstrated increased IL-8 production by C. neoformans in BEAS-2B cells [13,29,41]. In our results, the glucose supplementation reduced the IL-8 production in C. neoformans-infected cells compared to cells only infected, which is associate to downregulation of ERK1/2, and it is independent of NF-κB.…”

Section: Discussionmentioning

confidence: 87%

Effect of glucose supplementation on human bronchial epithelial cells infected with Cryptococcus neoformans

Emrich,

Bueno,

Santos

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

Diabetes could be a risk factor for cryptococcal meningitis in immunocompetent individuals. A model frequently used to understand cellular changes in diabetes consists of exposure to high concentrations of glucose in vitro. In the airways, epithelial cells are crucial for the establishment of cryptococcosis. We evaluated the effects of the combination of Cryptococcus neoformans and glucose concentrations (at 5mM or 15mM) on human bronchial epithelial cells (BEAS-2B) for 24h. No cytotoxic effect was observed in cells stimulated by C. neoformans and/or treated with glucose supplementation. C. neoformansincreased IL-6 and IL-8 productions and NF-kB, ERK1/2 and STAT3 activations in BEAS-2B cells when compared to control. In contrast to increased IL-6 production and STAT3 activation the IL-8 production and ERK1/2 activation markedly decreased upon culturing of cells stimulated by C. neoformans and supplemented by glucose when compared to cells only infected with C. neoformans. In addition, the glucose supplementation increased the internalization of C. neoformans in cells but reduced the percentage of C. neoformans on the surface and its growth. Conclusion: The glucose supplementation promoted pro- and anti-inflammatory responses in bronchial epithelial cells infected with C. neoformans.

show abstract

Cryptococcus neoformans in Association with Dermatophagoides pteronyssinus has Pro- (IL-6/STAT3 Overproduction) and Anti-inflammatory (CCL2/ERK1/2 Downregulation) Effects on Human Bronchial Epithelial Cells

et al. 2022

View full text Add to dashboard Cite

Effects of cigarette smoke extract on bronchial epithelial cells stimulated with Cryptococcus neoformans

Cited by 4 publications

References 71 publications

Human Airway Epithelium Responses to Invasive Fungal Infections: A Critical Partner in Innate Immunity

Human Airway Epithelium Responses to Invasive Fungal Infections: A Critical Partner in Innate Immunity

Effect of glucose supplementation on human bronchial epithelial cells infected with Cryptococcus neoformans

Cryptococcus neoformans in Association with Dermatophagoides pteronyssinus has Pro- (IL-6/STAT3 Overproduction) and Anti-inflammatory (CCL2/ERK1/2 Downregulation) Effects on Human Bronchial Epithelial Cells

Contact Info

Product

Resources

About