Effects of Circular RNA of Chicken Growth Hormone Receptor Gene on Cell Proliferation

Xu, Haidong; Leng, Qiying; Zheng, Jiahui; Adu-Asiamah, Patricia; Lin, Shudai; T, Li; Wang, Zhang; An, Lilong; Zhao, Z.H.; Zhang, Li

doi:10.3389/fgene.2021.598575

Cited by 3 publications

(3 citation statements)

References 28 publications

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“…Circ-GHR is reduced in the leg and pectoral muscles steadily from E13 to 7 w. It is positively correlated with GHR, and overexpressing Circ-GHR will promote myoblast proliferation. It is hypothesized that Circ-GHR may promote myoblast proliferation by regulating the expression of GHR mRNA and GH binding protein ( GHBP ), but it has been demonstrated to have no significant effect in DF-1 cell lines, so the definitive mechanism needs to be further explored [ 108 ].…”

Section: Circrna Modulates Skeletal Muscle In Domestic Chickensmentioning

confidence: 99%

Regulation of Non-Coding RNA in the Growth and Development of Skeletal Muscle in Domestic Chickens

Shi

et al. 2022

Genes

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Chicken is the most widely consumed meat product worldwide and is a high-quality source of protein for humans. The skeletal muscle, which accounts for the majority of chicken products and contains the most valuable components, is tightly correlated to meat product yield and quality. In domestic chickens, skeletal muscle growth is regulated by a complex network of molecules that includes some non-coding RNAs (ncRNAs). As a regulator of muscle growth and development, ncRNAs play a significant function in the development of skeletal muscle in domestic chickens. Recent advances in sequencing technology have contributed to the identification and characterization of more ncRNAs (mainly microRNAs (miRNAs), long non-coding RNAs (LncRNAs), and circular RNAs (CircRNAs)) involved in the development of domestic chicken skeletal muscle, where they are widely involved in proliferation, differentiation, fusion, and apoptosis of myoblasts and satellite cells, and the specification of muscle fiber type. In this review, we summarize the ncRNAs involved in the skeletal muscle growth and development of domestic chickens and discuss the potential limitations and challenges. It will provide a theoretical foundation for future comprehensive studies on ncRNA participation in the regulation of skeletal muscle growth and development in domestic chickens.

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Section: Circrna Modulates Skeletal Muscle In Domestic Chickensmentioning

confidence: 99%

Regulation of Non-Coding RNA in the Growth and Development of Skeletal Muscle in Domestic Chickens

Shi

et al. 2022

Genes

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“…The pre-RNA of the GHR gene can be back spliced to form a circular transcript (circGHR) in human, mouse and chicken [ 16 ]. We previously performed high-throughput RNA sequencing of chicken liver and muscle, finding that chicken circGHR could promote the proliferation of primary myoblasts [ 22 ]. In addition, we found that mouse circGHR was formed by backward splicing of exons 2 and 8 of the GHR gene.…”

Section: Introductionmentioning

confidence: 99%

The Mouse CircGHR Regulates Proliferation, Differentiation and Apoptosis of Hepatocytes and Myoblasts

Zhang

Lin

Jiao

et al. 2023

Genes

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The anterior pituitary gland of animals secretes growth hormone (GH) to bind to the growth hormone receptor (GHR) on the liver cell membrane through the blood circulation, thereby promoting the downstream gene insulin-like growth factor-1 (IGF1) expression, which is the canonical GH–GHR–IGF1 signaling pathway. Therefore, the amount of GHR and the integrity of its structure will affect animal growth and development. In the previous study, we found that the mouse GHR gene can transcribe a circular transcript named circGHR. Our group cloned the full-length of the mouse circGHR and analyzed its spatiotemporal expression profile. In this study, we further predicted the open reading frame of circGHR with bioinformatics, subsequently constructed a Flag-tagged protein vector and preliminarily verified its coding potential with western blot. Additionally, we found that circGHR could inhibit the proliferation of NCTC469 cells and has a tendency to inhibit cell apoptosis, while for C2C12 cells, it showed a tendency to inhibit cell proliferation and promote its differentiation. Overall, these results suggested that the mouse circGHR had the potential to encode proteins and affect cell proliferation, differentiation and apoptosis.

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“…3), indicating that GC proliferation could be induced. A review of the literature revealed that chicken circGHR positively regulates hepatocyte and myoblast proliferation (Xu et al 2021), and by binding to miR-204, circFNDC3AL increases the expression of BCL9 and encourages the growth and differentiation of SMSCS in chickens (Wei et al 2021). Moreover, circRNAs in follicular GCs are widely distributed at various developmental stages (Shen et al 2019).…”

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confidence: 99%

Expression spectrum of new circFBN1 in various tissues and follicles of Taihang chicken

ZHANG

et al. 2022

Indian J of Anim Sci

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In this study, the new circFBN1 derived from the FBN1 gene was identified. The expression spectrum of circFBN1 in various tissues and follicles of Taihang chickens was verified and analyzed. The effects on proliferation of GCs were checked. The results revealed that circFBN1 is indeed present and was differentially expressed in tissues and follicles and significantly promoted cell proliferation. In conclusion, our results suggested that circFBN1 could affect chicken follicular development by regulating the proliferation of GCs. These results will enable us recognize the molecular mechanisms in animal reproductive regulation.

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Effects of Circular RNA of Chicken Growth Hormone Receptor Gene on Cell Proliferation

Cited by 3 publications

References 28 publications

Regulation of Non-Coding RNA in the Growth and Development of Skeletal Muscle in Domestic Chickens

Regulation of Non-Coding RNA in the Growth and Development of Skeletal Muscle in Domestic Chickens

The Mouse CircGHR Regulates Proliferation, Differentiation and Apoptosis of Hepatocytes and Myoblasts

Expression spectrum of new circFBN1 in various tissues and follicles of Taihang chicken

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