Effects of co-administration of dopamine and vitamin C on ischaemia-reperfusion injury after experimental testicular torsion-detorsion in rats

Abstract

The objective of this study was to investigate the effects of dopamine as vasodilator, vitamin C as an antioxidant and combined administration of them on ischaemia-reperfusion (I-R) injury following testicular torsion (TT). Thirty adult male rats were divided into six groups each containing five rats. Testicular ischaemia was achieved by twisting the left testis for 4 h. Group 1 was for determination of the basal values. Group 2 had 4 h TT. Group 3 had 4 h TT and was then treated with dopamine. Group 4 had 4 h TT and was then treated with vitamin C. Group 5 had 4 h TT and was then treated with dopamine and vitamin C. Group 6 was designed as a sham operated group. Testicular torsion caused a significant decrease in the percentage of spermatogenesis and seminiferous tubules diameters compared with the control and sham groups. Administration of dopamine, vitamin C and their combination increased above mentioned parameters and decreased serum malondialehyde levels significantly. However, vitamin C had better results than the other treatments (P < 0.05). In conclusion, a potent antioxidant like vitamin C was found to be more effective than increasing blood flow by a vasodilator like dopamine on improving I-R injury following TT.

Ginkgo Biloba Ameliorates Subfertility Induced by Testicular Ischemia/Reperfusion Injury in Adult Wistar Rats: A Possible New Mitochondrial Mechanism

“…However, Robert et al [29] reported that testosterone did not show significant differences after 60 min of ischemia compared to basal values, and testosterone levels were decreased after 24 h of reperfusion. This discrepancy could be attributed to the suggestion of Azizollahi et al [30], who reported that the changes in biochemical parameters occurred earlier in comparison to morphologic changes; thus determination of testicular damage following unilateral testicular torsion by biochemical analysis could be more informative than the histologic and morphologic studies alone in the acute phase of I/R models.…”

The effects of carvedilol on ischemia-reperfusion injury in the rat testis

“…In other words, the changes in biochemical parameters occur earlier in comparison to morphologic changes, so for the evaluation of testicular damage after unilateral TT biochemical analysis would be more informative than the histologic and morphologic studies in the acute phase of I/R models (23). The aim of this study was to investigate the early biochemical changes in the testicular tissues of rats subjected to I/R injury and determine the efficacy of CVD administration on this deleterious condition, which is an antihypertensive, vasodilator agent but also has potent antioxidant activity (1,10,12,13).…”

“…In this context, to date various drugs and chemicals have been used to protect testes against I/R injury, such as N-acetyl cysteine, zofenopril, caffeic acid phenethyl ester (CAPE), erdosteine, L-carnitine, melatonin, edaravone, dopamine, vit C, trimetazidine and Ginkgo biloba (EGb 761) (2,4,5,7,8,17,(23)(24)(25). As a non--selective β 1, β 2 and α 1 -blocker agent, the mechanisms of antioxidant action of CVD has not been fully understood, but the greatest antioxidant activity has been attributed to its main metabolites, which are the hydroxylated compounds SB 211475, BM 910228 and SB 209995 (26).…”

Testicular torsion–detorsion and potential therapeutic treatments: A possible role for ischemic postconditioning

“…Ascorbic acid has been used as a treatment for testicular torsion–detorsion in a comparative study with dopamine . Treatment with ascorbic acid proved to be more beneficial in comparison with vasodilator dopamine, as it could significantly restore the decreased spermatogenesis and seminiferous tubule diameter, as well as significantly reducing the serum MDA levels .…”