Effects of cobalt and chromium ions on lymphocyte migration

Baskey, Stephen J.; Lehoux, Eric A.; Catelas, Isabelle

doi:10.1002/jor.23336

Cited by 15 publications

(9 citation statements)

References 47 publications

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“…Stock solutions of Co 2+ , Cr 3+ , and Ni 2+ were prepared fresh, as previously described [ 37 ]. Briefly, CoCl 2 •6H 2 O (≥99.5% purity; Fisher Scientific, Waltham, MA), CrCl 3 •6H 2 O (≥99.2% purity; Sigma-Aldrich, St Louis, MO) and NiCl 2 •6H 2 O (≥99.999% purity; Sigma-Aldrich) were dissolved in cell culture-grade water (Lonza, Walkersville, MD), and the solutions were sterilized by filtration through 0.2-μm pore size cellulose acetate syringe filters (VWR, Mississauga, ON).…”

Section: Methodsmentioning

confidence: 99%

Effects of metal ions on caspase-1 activation and interleukin-1β release in murine bone marrow-derived macrophages

Ferko

Catelas

2018

PLoS ONE

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Ions released from metal implants have been associated with adverse tissue reactions and are therefore a major concern. Studies with macrophages have shown that cobalt, chromium, and nickel ions can activate the NLRP3 inflammasome, a multiprotein complex responsible for the activation of caspase-1 (a proteolytic enzyme converting pro-interleukin [IL]-1β to mature IL-1β). However, the mechanism(s) of inflammasome activation by metal ions remain largely unknown. The objectives of the present study were to determine if, in macrophages: 1. caspase-1 activation and IL-1β release induced by metal ions are oxidative stress-dependent; and 2. IL-1β release induced by metal ions is NF-κB signaling pathway-dependent. Lipopolysaccharide (LPS)-primed murine bone marrow-derived macrophages (BMDM) were exposed to Co2+ (6–48 ppm), Cr3+ (100–500 ppm), or Ni2+ (12–96 ppm), in the presence or absence of a caspase-1 inhibitor (Z-WEHD-FMK), an antioxidant (L-ascorbic acid [L-AA]), or an NF-κB inhibitor (JSH-23). Culture supernatants were analyzed for caspase-1 by western blotting and/or IL-1β release by ELISA. Immunoblotting revealed the presence of caspase-1 (p20 subunit) in supernatants of BMDM incubated with Cr3+, but not with Ni2+ or Co2+. When L-AA (2 mM) was present with Cr3+, the caspase-1 p20 subunit was undetectable and IL-1β release decreased down to the level of the negative control, thereby demonstrating that caspase-1 activation and IL-1β release induced by Cr3+ was oxidative stress-dependent. ELISA demonstrated that Cr3+ induced the highest release of IL-1β, while Co2+ had no or limited effects. In the presence of Ni2+, the addition of L-AA (2 mM) also decreased IL-1β release, below the level of the negative control, suggesting that IL-1β release induced by Ni2+ was also oxidative stress-dependent. Finally, when present during both priming with LPS and activation with Cr3+, JSH-23 blocked IL-1β release, demonstrating NF-κB involvement. Overall, this study showed that while both Cr3+ and Ni2+ may be inducing inflammasome activation, Cr3+ is likely a more potent activator, acting through oxidative stress and the NF-κB signaling pathway.

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Section: Methodsmentioning

confidence: 99%

Effects of metal ions on caspase-1 activation and interleukin-1β release in murine bone marrow-derived macrophages

Ferko

Catelas

2018

PLoS ONE

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“…In addition, in the wear process of hip implants, Co NP may result in osteolytic inflammatory fluid formation. It is not entirely clear whether such immune responses are caused by a “metal-reactive” innate immune response mounted against metal debris or whether the adaptive immune system plays a role in frame of a “metal allergy”, which is also typically associated with contact dermatitis. , The nature of these local reactions is further complicated by the fact that Co-containing products are often mixed with other metals such as Ni. , Hints for an involvement also of cells of the adaptive arm of the immune system are derived from a study showing that Co 2+ ions stimulated migration of T cells, but not B cells, in vitro …”

Section: Immunological Effects Of Specific Metal Drugsmentioning

confidence: 99%

Metal Drugs and the Anticancer Immune Response

et al. 2018

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The immune system deploys a multitude of innate and adaptive mechanisms not only to ward off pathogens but also to prevent malignant transformation ("immune surveillance"). Hence, a clinically apparent tumor already reflects selection for those malignant cell clones capable of evading immune recognition ("immune evasion"). Metal drugs, besides their well-investigated cytotoxic anticancer effects, massively interact with the cancer-immune interface and can reverse important aspects of immune evasion. This topic has recently gained intense attention based on combination approaches with anticancer immunotherapy (e.g., immune checkpoint inhibitors), a strategy recently delivering first exciting results in clinical settings. This review summarizes the promising but still extremely fragmentary knowledge on the interplay of metal drugs with the fidelity of anticancer immune responses but also their role in adverse effects. It highlights that, at least in some cases, metal drugs can induce long-lasting anticancer immune responses. Important steps in this process comprise altered visibility and susceptibility of cancer cells toward innate and adaptive immunity, as well as direct impacts on immune cell populations and the tumor microenvironment. On the basis of the gathered information, we suggest initiating joint multidisciplinary programs to implement comprehensive immune analyses into strategies to develop novel and smart anticancer metal compounds.

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“…The undesired response to a MoM implant are frequently found to show soft tissue failure characterized by macrophage predominant infiltration with their massive intake of wear particles [15] . Metal ions (Co 2+ and Cr 3+ ) have been shown to enhance the migration of T lymphocytes independently of circulating cytokines or chemokines resulting in an accumulation of T lymphocytes in the periprosthetic tissues of some patients with CoCr-based implants [16] . However, the mechanism behind the macrophage migration in the presence of both wear debris and ions is largely unknown.…”

Section: Introductionmentioning

confidence: 99%