Effects of cold acclimation on sugar metabolism and sugar-related gene expression in tea plant during the winter season

Yue, Chuan; Cao, Hongli; Wang, Lu; Zhou, Yanhua; Huang, Yung‐yu; Hao, Xinyuan; Wang, Yuchun; Wang, Bo; Yang, Yajun; Wang, Xinchao

doi:10.1007/s11103-015-0345-7

Cited by 148 publications

(105 citation statements)

References 90 publications

(125 reference statements)

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“…During stress, the degradation of starch takes place in tea leaves for supporting respiration under low photosynthesis condition and to produce osmolytes (glucose or other sugars) as an active response against the osmotic stress (Yue et al, 2015; Liu et al, 2016). Moreover, transcriptome changes in DT led to cell wall disassembly, while the maintenance of cell wall integrity were observed in CT and CD.…”

Section: Discussionmentioning

confidence: 99%

Global Transcriptional Analysis Reveals the Complex Relationship between Tea Quality, Leaf Senescence and the Responses to Cold-Drought Combined Stress in Camellia sinensis

et al. 2016

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In field conditions, especially in arid and semi-arid areas, tea plants are often simultaneously exposed to various abiotic stresses such as cold and drought, which have profound effects on leaf senescence process and tea quality. However, most studies of gene expression in stress responses focus on a single inciting agent, and the confounding effect of multiple stresses on crop quality and leaf senescence remain unearthed. Here, global transcriptome profiles of tea leaves under separately cold and drought stress were compared with their combination using RNA-Seq technology. This revealed that tea plants shared a large overlap in unigenes displayed “similar” (26%) expression pattern and avoid antagonistic responses (lowest level of “prioritized” mode: 0%) to exhibit very congruent responses to co-occurring cold and drought stress; 31.5% differential expressed genes and 38% of the transcriptome changes in response to combined stresses were unpredictable from cold or drought single-case studies. We also identified 319 candidate genes for enhancing plant resistance to combined stress. We then investigated the combined effect of cold and drought on tea quality and leaf senescence. Our results showed that drought-induced leaf senescence were severely delayed by (i) modulation of a number of senescence-associated genes and cold responsive genes, (ii) enhancement of antioxidant capacity, (iii) attenuation of lipid degradation, (iv) maintenance of cell wall and photosynthetic system, (v) alteration of senescence-induced sugar effect/sensitivity, as well as (vi) regulation of secondary metabolism pathways that significantly influence the quality of tea during combined stress. Therefore, care should be taken when utilizing a set of stresses to try and maximize leaf longevity and tea quality.

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Section: Discussionmentioning

confidence: 99%

Global Transcriptional Analysis Reveals the Complex Relationship between Tea Quality, Leaf Senescence and the Responses to Cold-Drought Combined Stress in Camellia sinensis

et al. 2016

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“…Fructan, sucrose, fructose, and raffinose probably act as osmoprotectants to stabilize cellular membranes and a scavenger of ROS to protect plants against oxidative damage exposed to cold [13, 34, 35]. This was observed mainly in DX, where the greater accumulation of these sugars and higher induction of biosynthesis-related genes were over-represented at later phases of cold stress.…”

Section: Discussionmentioning

confidence: 99%

De novo transcriptome sequencing and gene expression profiling of Elymus nutans under cold stress

Miao

Shao

et al. 2016

BMC Genomics

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Background Elymus nutans Griseb., is an important alpine perennial forage of Pooideae subfamily with strong inherited cold tolerance. To get a deeper insight into its molecular mechanisms of cold tolerance, we compared the transcriptome profiling by RNA-Seq in two genotypes of Elymus nutans Griseb. the tolerant Damxung (DX) and the sensitive Gannan (GN) under cold stress.ResultsThe new E. nutans transcriptomes were assembled and comprised 200,520 and 181,331 transcripts in DX and GN, respectively. Among them, 5436 and 4323 genes were differentially expressed in DX and GN, with 170 genes commonly expressed over time. Early cold responses involved numerous genes encoding transcription factors and signal transduction in both genotypes. The AP2/EREBP famliy of transcription factors was predominantly expressed in both genotypes. The most significant transcriptomic changes in the later phases of cold stress are associated with oxidative stress, primary and secondary metabolism, and photosynthesis. Higher fold expressions of fructan, trehalose, and alpha-linolenic acid metabolism-related genes were detected in DX. The DX-specific dehydrins may be promising candidates to improve cold tolerance. Twenty-six hub genes played a central role in both genotypes under cold stress. qRT-PCR analysis of 26 genes confirmed the RNA-Seq results.ConclusionsThe stronger transcriptional differentiation during cold stress in DX explains its better cold tolerance compared to GN. The identified fructan biosynthesis, alpha-linolenic acid metabolism, and DX-specific dehydrin-related genes may provide genetic resources for the improvement of cold-tolerant characters in DX. Our findings provide important clues for further studies of the molecular mechanisms underlying cold stress responses in plants.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3222-0) contains supplementary material, which is available to authorized users.

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“…In tea plant, previous studies indicate AS is involved in important secondary metabolism pathways such as flavonoid and linalool biosynthesis [29,34]. Cold stress is one of the most severe abiotic stresses in tea plant, and a series of studies have been focusing on tea plant gene regulation during cold stress and cold acclimation [27,35,36]. Through cold acclimation, tea plants can alleviate the harm of low temperature and enhance their tolerance to cold stress [26].…”

Section: Discussionmentioning

confidence: 99%

“…These results were consistent with those of previous reports in other plant species [31]. During the sharp cooling treatment of tea plants, the numbers of AS events under low-temperatures [cold acclimation of 6 h at 10°C, day/night temperature (CS), cold acclimation of 7 days at 10/4°C, day/night temperature (CA1) and cold acclimation of 7 days at 4/0°C, day/ night temperature (CA2)] were significantly increased relative to AS events in those growing under normal temperature (NA, 25/20°C, day/night temperature); thus, the amount of CS (30,212), CA1 (30,325) and CA2 (30,552) was about 3000 more than NA (27,234), respectively. It is noteworthy that the number of AS events (24,616) tremendously decreased under temperature recovery condition compared with both CA treatment and NA groups, implying that many AS genes had adapted to low-temperature environment and resulted in reduced occurrence of AS events during DA (de-acclimation) of tea plant.…”

Section: Global Identification and Classification Of As Eventsmentioning

confidence: 99%

“…Tea plants are vulnerable to low temperatures in winter, especially in northern China [26]. In tea plant, the contents of sucrose, glucose and fructose were found to be constantly elevated during cold acclimation [27]. In addition, significantly increased expression of CBF (C-repeat-binding factor) and DHN (dehydrin) occurs during cold acclimation [28].…”

Section: Introductionmentioning

confidence: 99%

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Comprehensive profiling of alternative splicing landscape during cold acclimation in tea plant

Zhao

et al. 2020

BMC Genomics

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Background: Alternative splicing (AS) may generate multiple mRNA splicing isoforms from a single mRNA precursor using different splicing sites, leading to enhanced diversity of transcripts and proteins. AS has been implicated in cold acclimation by affecting gene expression in various ways, yet little information is known about how AS influences cold responses in tea plant (Camellia sinensis).Results: In this study, the AS transcriptional landscape was characterized in the tea plant genome using highthroughput RNA-seq during cold acclimation. We found that more than 41% (14,103) of genes underwent AS events. We summarize the possible existence of 11 types of AS events, including the four common types of intron retention (IR), exon skipping (ES), alternative 5′ splice site (A5SS), and alternative 3′ splice site (A3SS); of these, IR was the major type in all samples. The number of AS events increased rapidly during cold treatment, but decreased significantly following de-acclimation (DA). It is notable that the number of differential AS genes gradually increased during cold acclimation, and these genes were enriched in pathways relating to oxidoreductase activity and sugar metabolism during acclimation and de-acclimation. Remarkably, the AS isoforms of bHLH transcription factors showed higher expression levels than their full-length ones during cold acclimation. Interestingly, the expression pattern of some AS transcripts of raffinose and sucrose synthase genes were significantly correlated with sugar contents. Conclusion: Our findings demonstrated that changes in AS numbers and transcript expression may contribute to rapid changes in gene expression and metabolite profile during cold acclimation, suggesting that AS events play an important regulatory role in response to cold acclimation in tea plant.

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Effects of cold acclimation on sugar metabolism and sugar-related gene expression in tea plant during the winter season

Cited by 148 publications

References 90 publications

Global Transcriptional Analysis Reveals the Complex Relationship between Tea Quality, Leaf Senescence and the Responses to Cold-Drought Combined Stress in Camellia sinensis

Global Transcriptional Analysis Reveals the Complex Relationship between Tea Quality, Leaf Senescence and the Responses to Cold-Drought Combined Stress in Camellia sinensis

De novo transcriptome sequencing and gene expression profiling of Elymus nutans under cold stress

Comprehensive profiling of alternative splicing landscape during cold acclimation in tea plant

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