Effects of Colicin K on a Mutant of Escherichia coli Deficient in Ca2+, Mg2+-activated Adenosine Triphosphatase

Properties and characterization of binding protein dependent active transport of glutamine in isolated membrane vesicles of Escherichia coli

“…This was true in the presence of relatively high concentrations of the exogenous energy source (20 mM), as well as in vesicles that had been preincubated with the exogenous energy source. These observations suggest a role for A#H+ in glutamine transport in vesicles and agree with conclusions drawn previously by others (Singh & Bragg, 1977, 1979Plate et al, 1974;Plate, 1979). However, the data presented here do not conclusively rule out the possibility that these inhibitors might act by preventing the uptake and proper metabolism of the exogenous energy source by vesicles.…”

“…Effects of Uncouplers and Inhibitors of Respiration on Glutamine Transport. Several investigators have suggested that an electrochemical gradient of protons (Amh+) is required for shock-sensitive transport in E. coli (Plate et al, 1974;Singh & Bragg, 1977,1979Plate, 1979). Therefore, we studied the effects of various agents known to diminish AjttH+ on glutamine transport in isolated membrane vesicles (Figure 4).…”

Deenergization of nerve terminals by β-bungarotoxin

“…Secondarily, ATP is utilized in an attempt by the cell to repolarize its membrane. Under conditions in which the membrane (Ca2+,Mg2+)ATPase is made inactive in colicin-treated cells, the membranes become deenergized without an accompanying decrease in ATP levels (Feingold, 1970;Plate et al, 1974).…”

Periplasmic binding protein-dependent transport systems: the membrane-associated components

“…These include acetyl phosphate (Hong et 1979), NADPH (Gilson et al 1982), lipoic acid (Richarme 1985;Richarme & Heine 1986), and succinate (Hunt & Hong 1983). Arguments against a direct role for ATP have included the demonstration that, under certain conditions, a reduction in the cellular ATP pool does not lead to a corresponding reduction in transport (Plate et al 1974;Lieberman & Hong 1976;Ferenci et al 1977). Further confusion has arisen from findings that perturbation of the pmf can affect binding protein-dependent transport without necessarily having a major effect on ATP pools (Plate 1979;Singh & Bragg, 1979;Hunt & Hong, 1983;Ames 1986).…”