Effects of Collagen Concentration Variation toward Characteristics of Bacterial Cellulose-Collagen Biocomposites as Candidate of Artificial Dura Mater

Paramadini, Adanti Wido; Widiyanti, Prihartini; Rudyardjo, Djoni Izak

doi:10.4028/www.scientific.net/jbbbe.33.80

Cited by 3 publications

(1 citation statement)

References 9 publications

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“…Some collagen-based/BC composites have already been prepared following different methods while targeting diverse final uses such as in biomedical applications, − tissue regeneration, , or tissue-engineered scaffolds. − For instance, collagen has already been incorporated into BC films by immersion, − ,, which can be mediated by Fmoc-glycine (Fmoc-Gly) esterification, by adding collagen to the bacterial culture medium, or by diffusion under an electric field . The evaluation of BC films incorporating other biomaterials (hyaluronic acid, silk) indicates increased tensile strength.…”

Section: Introductionmentioning

confidence: 99%

Cell-Laden 3D Hydrogels of Type I Collagen Incorporating Bacterial Nanocellulose Fibers

Malandain,

Sanz-Fraile,

Farré

et al. 2023

ACS Appl. Bio Mater.

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There is a growing interest in developing natural hydrogel-based scaffolds to culture cells in a three-dimensional (3D) millieu that better mimics the in vivo cells' microenvironment. A promising approach is to use hydrogels from animal tissues, such as decellularized extracellular matrices; however, they usually exhibit suboptimal mechanical properties compared to native tissue and their composition with hundreds of different protein complicates to elucidate which stimulus triggers cell's responses. As simpler scaffolds, type I collagen hydrogels are used to study cell behavior in mechanobiology even though they are also softer than native tissues. In this work, type I collagen is mixed with bacterial nanocellulose fibers (BCf) to develop reinforced scaffolds with mechanical properties suitable for 3D cell culture. BCf were produced from blended pellicles biosynthesized from Komagataeibacter xylinus. Then, BCf were mixed with concentrated collagen from rat-tail tendons to form composite hydrogels. Confocal laser scanning microscopy and scanning electron microscopy images confirmed the homogeneous macro-and microdistribution of both natural polymers. Porosity analysis confirmed that BCf do not disrupt the scaffold structure. Tensile strength and rheology measurements demonstrated the reinforcement action of BCf (43% increased stiffness) compared to the collagen hydrogel while maintaining the same viscoelastic response. Additionally, this reinforcement of collagen hydrogels with BCf offers the possibility to mix cells before gelation and then proceed to the culture of the 3D cell scaffolds. We obtained scaffolds with human bone marrow-derived mesenchymal stromal cells or human fibroblasts within the composite hydrogels, allowing a homogeneous 3D viable culture for at least 7 days. A smaller surface shrinkage in the reinforced hydrogels compared to type I collagen hydrogels confirmed the strengthening of the composite hydrogels. These collagen hydrogels reinforced with BCf might emerge as a promising platform for 3D in vitro organ modeling, tissue-engineering applications, and suitable to conduct fundamental mechanobiology studies.

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Section: Introductionmentioning

confidence: 99%

Cell-Laden 3D Hydrogels of Type I Collagen Incorporating Bacterial Nanocellulose Fibers

Malandain,

Sanz-Fraile,

Farré

et al. 2023

ACS Appl. Bio Mater.

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Soy protein nanoparticles modified bacterial cellulose electrospun nanofiber membrane scaffold by ultrasound-induced self-assembly technique: characterization and cytocompatibility

Cai

Xiong

et al. 2019

Cellulose

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Dura Mater Substitutes in Neurosurgery: A Review

Fauza,

Al Fauzi,

Suroto

et al. 2024

MJMHS

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The outer layer of the meninges, known as duramater plays a critical role in protecting the brain. In cases where it is damaged due to head trauma or surgery there can be a risk of leakage that may have life threatening consequences. While suturing to repair the mater is not always feasible due to the potential for further leakage finding biomaterials capable of replacing the damaged dura becomes essential. During the 19th century initial attempts at using synthetic materials for dural replacement faced challenges as they were rejected by the immune system. However researchers have made progress by developing materials such as collagen based xenografts that undergo treatments to prevent adverse reactions. This review focuses on types of dural replacement materials, like autografts, allografts, xenografts and synthetics. It sheds light on efforts to find an ideal neurosurgical dural replacement material while also discussing associated challenges.

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Effects of Collagen Concentration Variation toward Characteristics of Bacterial Cellulose-Collagen Biocomposites as Candidate of Artificial Dura Mater

Cited by 3 publications

References 9 publications

Cell-Laden 3D Hydrogels of Type I Collagen Incorporating Bacterial Nanocellulose Fibers

Cell-Laden 3D Hydrogels of Type I Collagen Incorporating Bacterial Nanocellulose Fibers

Soy protein nanoparticles modified bacterial cellulose electrospun nanofiber membrane scaffold by ultrasound-induced self-assembly technique: characterization and cytocompatibility

Dura Mater Substitutes in Neurosurgery: A Review

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