Effects of collagen membranes and bone substitute differ in periodontal ligament cell microtissues and monolayers

Janjić, Klara; Agis, Hermann; Moritz, Andreas; Rausch‐Fan, Xiaohui; Andrukhov, Oleh

doi:10.1002/jper.21-0225

Cited by 9 publications

(10 citation statements)

References 50 publications

(78 reference statements)

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“…COL1α1 is a major component of the soft tissue ECM and is a known wound-healing gene. 38 TGF-β1, a cytokine, is involved in the wound healing process which promotes fibroblast proliferation and collagen synthesis. 39 Excessive expression of TGF-β1 causes excessive synthesis of collagen and fibroblasts and induces the expression of connexin 43, which promotes hypertrophic scar.…”

Section: Discussionmentioning

confidence: 99%

Orodispersible hyaluronic acid film delivery for oral wound healing in rats

Lee

Nam

et al. 2022

Journal of Dental Sciences

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Section: Discussionmentioning

confidence: 99%

Orodispersible hyaluronic acid film delivery for oral wound healing in rats

Lee

Nam

et al. 2022

Journal of Dental Sciences

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“…Type I collagen is the major component of the extracellular matrix (ECM) in periodontal tissues, and it acts as a signaling molecule to regulate cell proliferation, differentiation, and migration. 16 As shown in Figure 4 , the addition of CHA and H-PRF stimulated the gene expression of COL1A1, and COL1A1 gene expression was higher in the PRF group than in the CHA group. Moreover, the CHA-PRF group showed the highest expression level of COL1A1.…”

Section: Resultsmentioning

confidence: 83%

Novel approach to soft tissue regeneration: in vitro study of compound hyaluronic acid and horizontal platelet-rich fibrin combination

QIU,

SHEN,

WEI

et al. 2024

J. Appl. Oral Sci.

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Objective This study aims to develop a compound biomaterial to achieve effective soft tissue regeneration. Methodology Compound hyaluronic acid (CHA) and liquid horizontal-platelet-rich fibrin (H-PRF) were mixed at a ratio of 1:1 to form a CHA-PRF gel. Human gingival fibroblasts (HGFs) were used in this study. The effect of CHA, H-PRF, and the CHA-PRF gel on cell viability was evaluated by CCK-8 assays. Then, the effect of CHA, H-PRF, and the CHA-PRF gel on collagen formation and deposition was evaluated by qRT‒PCR and immunofluorescence analysis. Finally, qRT‒PCR, immunofluorescence analysis, Transwell assays, and scratch wound-healing assays were performed to determine how CHA, H-PRF, and the CHA-PRF gel affect the migration of HGFs. Results The combination of CHA and H-PRF shortened the coagulation time of liquid H-PRF. Compared to the pure CHA and H-PRF group, the CHA-PRF group exhibited the highest cell proliferation at all time points, as shown by the CCK-8 assay. Col1a and FAK were expressed at the highest levels in the CHA-PRF group, as shown by qRT‒PCR. CHA and PRF could stimulate collagen formation and HGF migration, as observed by fluorescence microscopy analysis of COL1 and F-actin and Transwell and scratch healing assays. Conclusion The CHA-PRF group exhibited greater potential to promote soft tissue regeneration by inducing cell proliferation, collagen synthesis, and migration in HGFs than the pure CHA or H-PRF group. CHA-PRF can serve as a great candidate for use alone or in combination with autografts in periodontal or peri-implant soft tissue regeneration.

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“…Collagen (Col) is an essential component of the extracellular matrix, with a variety of cell signaling binding sites and excellent biocompatibility, and is widely used in the development of periodontal tissue engineering scaffolds [ 18 , 46 , 47 ]. Collagen can support the adhesion, growth, proliferation, and directed differentiation of functional cells associated with periodontal regeneration [ 48 ]. However, collagen-based hydrogels have poor mechanical properties and stability, and terrestrial animal-derived collagen may cause immune reactions.…”

Section: Components Of Hydrogel In Periodontal Tissue Regenerationmentioning

confidence: 99%

Advances of Hydrogel Therapy in Periodontal Regeneration—A Materials Perspective Review

Mao-xue

Yang

et al. 2022

Gels

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Hydrogel, a functional polymer material, has emerged as a promising technology for therapies for periodontal diseases. It has the potential to mimic the extracellular matrix and provide suitable attachment sites and growth environments for periodontal cells, with high biocompatibility, water retention, and slow release. In this paper, we have summarized the main components of hydrogel in periodontal tissue regeneration and have discussed the primary construction strategies of hydrogels as a reference for future work. Hydrogels provide an ideal microenvironment for cells and play a significant role in periodontal tissue engineering. The development of intelligent and multifunctional hydrogels for periodontal tissue regeneration is essential for future research.

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Effects of collagen membranes and bone substitute differ in periodontal ligament cell microtissues and monolayers

Cited by 9 publications

References 50 publications

Orodispersible hyaluronic acid film delivery for oral wound healing in rats

Orodispersible hyaluronic acid film delivery for oral wound healing in rats

Novel approach to soft tissue regeneration: in vitro study of compound hyaluronic acid and horizontal platelet-rich fibrin combination

Advances of Hydrogel Therapy in Periodontal Regeneration—A Materials Perspective Review

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