Effects of Collagen Tripeptide Supplement on Photoaging and Epidermal Skin Barrier in UVB-exposed Hairless Mice

Pyun, Hee Bong; Kim, Minji; Park, Jieun; Sakai, Yasuo; Numata, Noriaki; Shin, Jin Yeong; Shin, Hyun Jung; Kim, Do Un; Hwang, Jae Kwan

doi:10.3746/pnf.2012.17.4.245

Cited by 97 publications

(70 citation statements)

References 26 publications

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“…Repetitive UVB irradiation of hairless mice has been used previously to evaluate test compounds on skin photoageing, photodamage, inflammation and tumor development . UVB–induced erythema also has been used clinically in healthy volunteers to assess the photoprotective and anti‐inflammatory activity of potential therapeutic agents .…”

Section: Discussionmentioning

confidence: 99%

Reduction in ultraviolet B light–induced erythema by oxymetazoline and brimonidine is mediated by different α‐adrenoceptors

Hsia

Tian

Gil

2018

Experimental Dermatology

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When applied topically, oxymetazoline and brimonidine reduce the persistent facial erythema of rosacea; this effect is mediated by cutaneous vasoconstriction induced by postsynaptic activation of α-adrenoceptors. We investigated the α-adrenergic pharmacology of oxymetazoline and brimonidine. Functional activity on α-adrenoceptors was evaluated in vitro in HEK293 cells stably expressing single receptor subtypes using a fluorometric imaging plate reader Ca influx assay. Oxymetazoline was an α -adrenoceptor agonist with partial α -adrenoceptor activity, whereas brimonidine was a highly selective full α -adrenoceptor agonist. In vivo pharmacology was investigated in a mouse model of ultraviolet B light (UVB)-induced skin erythema. To selectively inhibit α-adrenoceptor subtypes, mice were injected with prazosin (an α -selective antagonist) or rauwolscine (an α -selective antagonist) following UVB exposure. Oxymetazoline cream 1.0%, brimonidine gel 0.33% or vehicle control was applied topically, and erythema was measured using a chromameter. Oxymetazoline and brimonidine reduced UVB-induced erythema compared with vehicle control (P < .01). The effect of oxymetazoline was impaired in prazosin-pretreated but not rauwolscine-pretreated mice. Conversely, the effect of brimonidine was impaired in rauwolscine-pretreated but not prazosin-pretreated mice. These data suggest that while oxymetazoline and brimonidine produce cutaneous vasoconstriction, they do so through different α-adrenergic mechanisms, with oxymetazoline primarily acting via α -adrenoceptors and brimonidine acting via α -adrenoceptors.

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Section: Discussionmentioning

confidence: 99%

Reduction in ultraviolet B light–induced erythema by oxymetazoline and brimonidine is mediated by different α‐adrenoceptors

Hsia

Tian

Gil

2018

Experimental Dermatology

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“…Also, collagen hydrolysates from Chum Salmon skin were found to stimulate collagen synthesis and decrease collagen degradation in a dose-dependent manner [7]. Moreover, Gly-X-Y tripeptides derived from collagen hydrolysates also showed anti-aging properties such as increased skin moisture content, increased skin elasticity, and decreased MMP activities in UV-irradiated hairless mice [12].…”

Section: Introductionmentioning

confidence: 97%

Anti-aging potential of fish collagen hydrolysates subjected to simulated gastrointestinal digestion and Caco-2 cell permeation

Han

Lee

et al. 2019

JABC

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The objectives of this study were to evaluate the antiaging effects and investigate the effect of simulated gastrointestinal (GI) digestion on the anti-aging properties and intestinal permeation of the potential fish collagen hydrolysates (FCH). Therefore, procollagen synthesis, matrix metalloproteinase-1 (MMP-1) production, and Caco-2 cell permeability were analyzed before and after in vitro digestion for FCHs, low-molecular weight fractions (<1 kDa), and high molecular weight fractions (>1 kDa). After being subjected to GI digestion, the level of MMP-1 inhibition was maintained, although the procollagen production was significantly (>20%) lower with all samples. Also, the digested FCHs and their <1 kDa fraction yielded 9.1 and 13.8% increased peptide transport, respectively, compared to undigested samples. Based on the effective intestinal permeation and high digestive enzyme stability, the <1 kDa fraction of FCHs is a potential bioactive material suitable for anti-aging applications in the food and cosmetics industries.

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“…These CTP are produced in several sequences, including Gly-Pro-Hyp, Gly-Pro-Ala, and Gly-Ala-Hyp. CTP can help in healing bone 16,17) and has several beneficial effects on the skin, including protection against photoaging caused by UV-B irradiation 18) and anti-wrinkle and anti-pruritus effects, 19) and enhancing hyaluronic acid synthesis. 19) In addition, a hydrolysate of chicken collagen peptides rich in Gly-Pro-Hyp can affect osteoporosis.…”

mentioning

confidence: 99%

Absorption and plasma kinetics of collagen tripeptide after peroral or intraperitoneal administration in rats

Yamamoto¹,

Hayasaka²,

Deguchi³

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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Collagen tripeptide (CTP) is a collagen-derived compound containing a high concentration of tripeptides with a Gly-X-Y sequence. In this study, the concentrations and metabolites of CTP were monitored in rat plasma after its administration. We performed a quantitative analysis using high-performance liquid chromatography tandem mass spectrometry according to the isotopic dilution method with stable isotopes. We confirmed that the tripeptides Gly-Pro-Hyp, Gly-Pro-Ala, and Gly-Ala-Hyp were transported into the plasma. Dipeptides, which are generated by degradation of the N- or C-terminus of the tripeptides Gly-Pro-Hyp, Gly-Pro-Ala, and Gly-Ala-Hyp, were also present in plasma. The plasma kinetics for peroral and intraperitoneal administration was similar. In addition, tripeptides and dipeptides were detected in no-administration rat blood. The pharmacokinetics were monitored in rats perorally administered with Gly-[(3)H]Pro-Hyp. Furthermore, CTP was incorporated into tissues including skin, bone, and joint tissue. Thus, administering collagen as tripeptides enables efficient absorption of tripeptides and dipeptides.

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Effects of Collagen Tripeptide Supplement on Photoaging and Epidermal Skin Barrier in UVB-exposed Hairless Mice

Cited by 97 publications

References 26 publications

Reduction in ultraviolet B light–induced erythema by oxymetazoline and brimonidine is mediated by different α‐adrenoceptors

Reduction in ultraviolet B light–induced erythema by oxymetazoline and brimonidine is mediated by different α‐adrenoceptors

Anti-aging potential of fish collagen hydrolysates subjected to simulated gastrointestinal digestion and Caco-2 cell permeation

Absorption and plasma kinetics of collagen tripeptide after peroral or intraperitoneal administration in rats

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