Effects of commonly used nitrification inhibitors—dicyandiamide (DCD), 3,4-dimethylpyrazole phosphate (DMPP), and nitrapyrin—on soil nitrogen dynamics and nitrifiers in three typical paddy soils

Zhou, Xue; Wang, Shuwei; Ma, Siqi; Zheng, Xin; Wang, Zhiyuan; Lu, Chunhui

doi:10.1016/j.geoderma.2020.114637

Cited by 88 publications

(28 citation statements)

References 100 publications

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“…This result is consistent with the previous reports that the nitrification inhibitors played an important role in inhibiting nitrification in different soils (Xu et al 2001;Gong et al 2013;Zhou et al 2020). Our study shows that both the soil incubation and pot experiment accumulated more ammonium nitrogen and lowered the loss of nitrate in soils because of the presence of nitrification inhibitors, thus increasing the accumulation of ammonium-and nitrate-nitrogen in soils (Fig.…”

Section: Nitrification Inhibitors Remarkably Inhibited the Nitrification Of Ammonium In Soilsupporting

confidence: 93%

A novel amended nitrification inhibitor confers an enhanced suppression role in the nitrification of ammonium in soil

Guo

Wang²,

Dian-bo

et al. 2022

J Soils Sediments

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Purpose Nitrification inhibitor plays an important regulatory role in inhibiting the nitrification of ammonium in soils. However, most of nitrification inhibitors lack the sustainable effects in suppressing the nitrification of ammonium. In this study, a novel DMS nitrification inhibitor was prepared and tested to explore its lasting effect of nitrification suppression in black soil. Materials and methods Both culture experiments and field trial were performed in black soils. Three kinds of nitrification inhibitors (NIs), dicyandiamide (DCD) with low bioactivity, 3,4-dimethylpyrazole phosphate (DMPP) with high bioactivity, and a novel 3,4-dimethylpyrazole sulfate zinc (DMS) with long half-life, were applied into soils, respectively, and the abundance changes of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) were investigated; then, the accumulation changes of inorganic nitrogen, nitrogen use efficiency, and crop yields were furtherly evaluated. Results and discussions A novel DMS nitrification inhibitor with high activity and long half-life maintained a persistent effect of nitrification suppression, and remarkably increased the accumulation of ammonium nitrogen in soil, thus improving nitrogen use efficiency and crop yields. This study implies that lowering the nitrogen loss of nitrification-triggered in soil is of great importance for improving nitrogen use efficiency. Conclusions This study provided an insight into the sustainable nitrification suppression of a novel DMS nitrification inhibitor under excessive application of nitrogen fertilizer in black soils. Compared with improving the activity, reasonably prolonging the validity of nitrification inhibitors in soil is a more important strategy increasing the sustainable effects of nitrification inhibition, and the survival period of nitrification inhibitors in soil should be a crucial factor improving nitrogen use efficiency.

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Section: Nitrification Inhibitors Remarkably Inhibited the Nitrification Of Ammonium In Soilsupporting

confidence: 93%

A novel amended nitrification inhibitor confers an enhanced suppression role in the nitrification of ammonium in soil

Guo

Wang²,

Dian-bo

et al. 2022

J Soils Sediments

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“…The effectiveness of NIs depends on environmental and soil conditions like soil moisture, carbon content, and pH (Zhang et al, 2020;Zhou et al, 2020). From soils receiving liquid manure additions, nitrapyrin and DMPSA reduced N 2 O emissions 60 and 56% at the 60 and 80% WFPS of the Gray Luvisolic soil, respectively, reductions similar to the 58% found at the 60% WFPS of the Black Chernozemic (BC) soil.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, the application of nitrification inhibitors (NIs) mixed with the slurry has been proposed as an alternative to reduce its environmental impact and enhance the N availability for crop nutrition (Owusu-Twum et al, 2017;Recio et al, 2018). NIs suppress the metabolic activity of ammonia-oxidizing bacteria by inhibition of the subunit B of ammonia-monooxygenase (AMO), the first enzyme implicated in the oxidation of ammonium (NH 4 + ) to NO 2 − in soils, reducing the nitrification rate and potentially its loss as NO 3 − or N 2 O afterward (Ruser and Schulz, 2015;Beeckman et al, 2018;Zhou et al, 2020). Nevertheless, the increase in the NH 4 + associated with the use of NIs could enhance the risk of NH 3 volatilization, in particular when the slurry is not incorporated into the soil (Kim et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Using the Nitrification Inhibitor Nitrapyrin in Dairy Farm Effluents Does Not Improve Yield-Scaled Nitrous Oxide and Ammonia Emissions but Reduces Methane Flux

Castillo

Méndez

Elizondo-Salazar

et al. 2021

Front. Sustain. Food Syst.

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The application of dairy farm effluents (DFE) without previous treatment in paddocks was intensified due to the approval of this practice in Costa Rican legislation since 2012. Applying DFE instead of synthetic N fertilizer in grasslands is an opportunity to reach a circular economy; however, this practice increases the risk of emissions of nitrous oxide (N2O), methane (CH4), and ammonia (NH3), which contribute to global warming. A field experiment was carried out using a permanent grassland (90% Star grass and 10% Kikuyo grass) to simultaneously assess the effect of nitrapyrin on yield-scaled emissions of NH3, CH4, and N2O. The experiment lasted for 5 months in 2017, based on a randomized complete block design, including three treatments of control (CK) without N application, surface application of DFE with nitrapyrin (SNI), and without nitrapyrin (S). Total N applied was 149 ± 12 kg N ha−1 for both S and SNI treatments split into five applications. CH4 emissions from S, SNI, and CK showed a high temporal variation. Daily fluxes of CH4 from SNI were significantly lower than those of S in August (P < 0.05). Cumulative emissions of CH4, the majority produced in the soil, ranged from 4 to 168 g ha−1 for S, and from −13 to 88 g ha−1 for SNI. The ratio between the N2O cumulative emissions and the N applied as DFE were 1.6 ± 0.5 and 1.7 ± 0.2% for S and SNI, respectively. NH3 volatilization potential was very low (i.e., 0.6 ± 0.2% of the N applied). Under the prevailing experimental conditions, no significant difference between yield-scaled NH3 and N2O emissions were found between S and SNI, suggesting that nitrapyrin may not be a viable mitigation option for gaseous N losses from DFE application in Costa Rican grasslands in rainy season.

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“…Straw incorporation doubled the abundance of nosZ gene, the only known biological link that reduces N2O emissions, in an acidic soil (Miller et al 2012), but potentially increase the emissions of N2O from cropland soils across large spatial scales (Zhao et al 2020). Previous study showed that DCD stressed paddy soil N2O emission via inhibiting the metabolic activity of AOB rather than AOA (Zhou et al 2020). DCD was also reported to potentially reduce denitrification activity via reducing denitrification substrate (NO3 -) concentration rather than the abundance of nirK gene in a paddy soil (Meng et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Effect of straw incorporation and nitrification inhibitor on nitrous oxide emission in various cropland soils and its microbial mechanism

Zhang

Wang

et al. 2021

Preprint

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Nitrification inhibitor and straw incorporation are widely used to improve crop nitrogen use efficiency in agricultural soil, but their effects on nitrous oxide (N2O) emission across different soil types and the microbial mechanisms remain less understood. In this study, we used controlled experiment and DNA-based molecular analysis to study how nitrification inhibitor (dicyandiamide, DCD) and straw incorporation affect soil nitrogen balance, N2O emission and microbial nitrifiers/denitrifers in three distinct agricultural soils (the black, fluvo-aquic and red soils) across China. Both DCD and straw incorporation improved nitrogen balance by increasing NH4+ and decreasing NO3- in all soils. DCD tended to decrease N2O emission from all soils especially the Fluvo-aquic one, while straw incorporation reduced N2O emission only in the fluvo-aquic soil but increased N2O emission in the other two especially the red soil (by ~600%). T-RFLP analysis revealed that the denitriers community structure are distinct among the three soils, but was not strongly affected by DCD or straw incorporation. qPCR analysis revealed that DCD or straw incorporation had no significant effect on nitrifier abundance, but increased nitrous oxide reductase nosZ gene abundance in the black/fluvo-aquic soil rather than the red soil. Structural equational modelling further confirmed that, when accounting for treatments and soil properties, nosZ gene abundance is the only biological factor significantly determined N2O emission in different soil types. Taken together, our work advanced the knowledge on the agricultural practices and N2O emission in cropland soils, and suggested that straw incorporation may not be a good choice for the red and black soil areas; management practices should be used as per soil type to balance between nitrogen use efficiency and N2O emission.

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Effects of commonly used nitrification inhibitors—dicyandiamide (DCD), 3,4-dimethylpyrazole phosphate (DMPP), and nitrapyrin—on soil nitrogen dynamics and nitrifiers in three typical paddy soils

Cited by 88 publications

References 100 publications

A novel amended nitrification inhibitor confers an enhanced suppression role in the nitrification of ammonium in soil

A novel amended nitrification inhibitor confers an enhanced suppression role in the nitrification of ammonium in soil

Using the Nitrification Inhibitor Nitrapyrin in Dairy Farm Effluents Does Not Improve Yield-Scaled Nitrous Oxide and Ammonia Emissions but Reduces Methane Flux

Effect of straw incorporation and nitrification inhibitor on nitrous oxide emission in various cropland soils and its microbial mechanism

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