2020
DOI: 10.1155/2020/2814015
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Effects of Compressive and Tensile Strain on Macrophages during Simulated Orthodontic Tooth Movement

Abstract: During orthodontic tooth movement (OTM) to therapeutically correct the position of misaligned teeth, thus improving oral health and quality of life, fibroblasts, macrophages, and other immune cells within the periodontal ligament (PDL), which connects a tooth to its surrounding bone, are exposed to compressive and tensile strain. While it is known that PDL fibroblasts are critically involved in the biological regulation of OTM by a mechanotransductively triggered release of cytokines, it is unclear whether mac… Show more

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Cited by 28 publications
(60 citation statements)
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“…Damage-associated molecular patterns are released by necrotic cell death so that the inflammatory cascade can begin [ 28 ]. As an initial reaction, cytokines such as TNF and IL-6 are secreted [ 7 ]. Both inflammation markers are significantly involved in OTM but are not known classical HIF-1 α target genes [ 29 ].…”
Section: Discussionmentioning
confidence: 99%
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“…Damage-associated molecular patterns are released by necrotic cell death so that the inflammatory cascade can begin [ 28 ]. As an initial reaction, cytokines such as TNF and IL-6 are secreted [ 7 ]. Both inflammation markers are significantly involved in OTM but are not known classical HIF-1 α target genes [ 29 ].…”
Section: Discussionmentioning
confidence: 99%
“…Therefore, approximately 250,000 RAW264.7 macrophages per ml were seeded onto sterile 6-well cell culture plates (353046, Omnilab) and cultivated for 24 h before the start of the experiment. Compressive force application was performed by using glass plates with a defined weight of 2 g/cm 2 for additional 24 h under normoxic or hypoxic conditions according to an established and published model [ 7 , 16 , 17 ] ( Figure 1 ). Hypoxic conditions were achieved by using a GasPak EZ gas development bag system with an indicator (260683, BD Biosciences) according to the manufacturer's instructions increasing CO 2 to at least 10% within 24 h. Therefore, we also measured pH (Seven Easy in combination with Inlab Expert pro electrode, Mettler Toledo) and detected a slight, but significant, effect of CO 2 on pH in the cell culture media (Supplemental figure 1 ).…”
Section: Methodsmentioning
confidence: 99%
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