Effects of Concentrations and Exposure Times of Sodium Hypochlorite on Dentin Deproteination: Attenuated Total Reflection Fourier Transform Infrared Spectroscopy Study

Hu, Xiaoli; Peng, Yanwen; Sum, CheePeng; Ling, Junqi

doi:10.1016/j.joen.2010.08.035

Cited by 46 publications

(50 citation statements)

References 28 publications

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“…Studies have shown that the use of high concentration of sodium hypochlorite, a commonly used endodontic irrigant, increases collagen degradation, dentin deproteination, and reduces flexural strength of dentin (33,34). Similarly, long-term dressing of calcium hydroxide also weakens root canal treated teeth (35).…”

Section: Discussionmentioning

confidence: 99%

N-Acetylcysteine Inhibits Growth and Eradicates Biofilm of Enterococcus faecalis

Quah

Lui

et al. 2012

Journal of Endodontics

102

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Section: Discussionmentioning

confidence: 99%

N-Acetylcysteine Inhibits Growth and Eradicates Biofilm of Enterococcus faecalis

Quah

Lui

et al. 2012

Journal of Endodontics

102

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“…A key effect of NaOCl on dentine is denaturation of dentinal collagen and the organic component of the dentine matrix (Hu et al . , Zhang et al . ,, Tartari et al .…”

Section: Introductionmentioning

confidence: 99%

Proof‐of‐concept study to establish an in situ method to determine the nature and depth of collagen changes in dentine using Fourier Transform Infra‐Red spectroscopy after sodium hypochlorite irrigation

Morgan

Odlyha

et al. 2018

Int Endodontic J

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“…A key effect of NaOCl on dentine is denaturation of dentinal collagen and the organic component of the dentine matrix (Hu et al . , Zhang et al . ,b, Tartari et al .…”

Section: Introductionmentioning

confidence: 99%

“…Fourier Transform Infrared (FTIR) spectroscopy has been successfully employed to assess changes caused by NaOCl within dentine (di Renzo et al 2001a,b, Amarie et al 2012, Tartari et al 2017, Ram ırez-Bommer et al 2018. A key effect of NaOCl on dentine is denaturation of dentinal collagen and the organic component of the dentine matrix (Hu et al 2010, Zhang et al 2010a,b, Tartari et al 2016. By monitoring changes in the IR absorption frequencies following NaOCl exposure, it is possible to assess the depth of any changes due to root canal irrigation (Morgan et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Influence of root maturity or periodontal involvement on dentinal collagen changes following NaOCl irrigation: an ex vivo study

Browne

Odlyha

et al. 2019

Int Endodontic J

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Aim To refine a FTIR protocol for detection of NaOCl‐induced dentinal collagen changes using an ex vivo irrigation model, and to apply it to determine the collagen change within 0.5 mm of canal or root surfaces, with or without mature roots or periodontal involvement. Methodology The root canals of extracted human roots were irrigated with control saline (n = 3) or 5% NaOCl (n = 3) and sectioned into transverse disks for FTIR analyses, 0.5 mm from both the canal lumen and root surface, before and after surface treatment with 17% EDTA. Amide I/phosphate and amide II/phosphate absorbance ratios were compared using the Wilcoxon sign rank test. Mature roots without periodontal involvement were irrigated with: saline (n = 7), 5% NaOCl (n = 7) or 5% NaOCl + 17% EDTA (n = 7); those with periodontal involvement (n = 7) or immature roots (n = 7) were irrigated with 5% NaOCl. Dentine disks were then prepared for FTIR analyses. The effects of irrigant/root maturity/periodontal involvement were analysed using linear mixed models. Results FTIR analyses of the irrigated samples revealed a significant (P < 0.05) reduction in collagen bands near the canal lumen after NaOCl irrigation using surface EDTA‐treated samples. Irrigation with the test solutions resulted in significant (P < 0.0001) dentinal collagen changes in the mature roots, whilst those in the immature roots were significantly (P < 0.05) greater compared with the mature roots with or without periodontal involvement; but there was no difference between the latter groups. Conclusion EDTA surface treatment of polished dentine surfaces enhanced FTIR detection of NaOCl‐induced collagen changes. Both root maturity and irrigation protocol influenced the ability of NaOCl to alter dentinal collagen up to 0.5 mm from the canal lumen.

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Effects of Concentrations and Exposure Times of Sodium Hypochlorite on Dentin Deproteination: Attenuated Total Reflection Fourier Transform Infrared Spectroscopy Study

Cited by 46 publications

References 28 publications

N-Acetylcysteine Inhibits Growth and Eradicates Biofilm of Enterococcus faecalis

N-Acetylcysteine Inhibits Growth and Eradicates Biofilm of Enterococcus faecalis

Proof‐of‐concept study to establish an in situ method to determine the nature and depth of collagen changes in dentine using Fourier Transform Infra‐Red spectroscopy after sodium hypochlorite irrigation

Influence of root maturity or periodontal involvement on dentinal collagen changes following NaOCl irrigation: an ex vivo study

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