Effects of connexin-mimetic peptides on perfusion pressure in response to phenylephrine in isolated, perfused rat kidneys

Piao, Honglan; Sato, Ayako; Nozawa, Yoshinori; Sun, Wei; Morioka, Tetsuo; Oite, Takashi

doi:10.1007/s10157-010-0382-0

Cited by 9 publications

(16 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Given that Panx3 has been shown to release ATP, a role for TGF regulation via purinergic signaling cascades cannot be excluded [16]. In contrast to previous studies [21,55,56], Piao and colleagues showed a pivotal role of Cx43 in perfusion pressure [37]. These discrepancies in the involvement of different Cx species in renal autoregulatory mechanisms could be related to different experimental settings and the stability of different blocking peptides.…”

Section: Autoregulation Of Renal Blood Flowcontrasting

confidence: 46%

“…The authors showed that Cx43 plays a pivotal role in regulating renal vascular resistance, as administration of 43 Gap26 significantly elevated perfusion pressure. In addition, infusion of 40 Gap27 considerably suppressed the increase in perfusion pressure induced by phenylephrine, indicating that Cx40 attenuates phenylephrine-induced vasoconstriction [37]. Finally, blocking peptides directed against Cx37, Cx40, Cx43 or Cx45 had no effect on conducted calcium responses in isolated rat interlobular arteries [38].…”

Section: Vascular-conducted Responsesmentioning

confidence: 93%

See 1 more Smart Citation

Functional roles of connexins and pannexins in the kidney

Abed

Kavvadas

Chadjichristos

2015

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

Kidneys are highly complex organs, playing a crucial role in human physiopathology, as they are implicated in vital processes, such as fluid filtration and vasomotor tone regulation. There is growing evidence that gap junctions are major determinants of renal physiopathology. It has been demonstrated that their expression or channel activity may vary depending on physiological and pathological situations within distinct renal compartments. While some studies have focused on the role of connexins in renal physiology, our knowledge regarding the functional relevance of pannexins is still very limited. In this paper, we provide an overview of the involvement of connexins, pannexins and their channels in various physiological processes related to different renal compartments.

show abstract

Section: Autoregulation Of Renal Blood Flowcontrasting

confidence: 46%

Section: Vascular-conducted Responsesmentioning

confidence: 93%

Functional roles of connexins and pannexins in the kidney

Abed

Kavvadas

Chadjichristos

2015

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

show abstract

“…Thus, we strongly suggest that more than one type of Cx hemichannel is involved in the mHBSS-induced enhanced neuronal activity. Nevertheless, Cx43 appears to have a prominent role because the mHBSS-induced enhanced neuronal activity was drastically inhibited by Gap27, which is a well-known Cx43 hemichannel blocker (Piao et al, 2011; Wang et al, 2012). …”

Section: Discussionmentioning

confidence: 99%

Opening of pannexin- and connexin-based channels increases the excitability of nodose ganglion sensory neurons

Retamal

Alcayaga

Verdugo

et al. 2014

Front. Cell. Neurosci.

View full text Add to dashboard Cite

Satellite glial cells (SGCs) are the main glia in sensory ganglia. They surround neuronal bodies and form a cap that prevents the formation of chemical or electrical synapses between neighboring neurons. SGCs have been suggested to establish bidirectional paracrine communication with sensory neurons. However, the molecular mechanism involved in this cellular communication is unknown. In the central nervous system (CNS), astrocytes present connexin43 (Cx43) hemichannels and pannexin1 (Panx1) channels, and the opening of these channels allows the release of signal molecules, such as ATP and glutamate. We propose that these channels could play a role in glia-neuron communication in sensory ganglia. Therefore, we studied the expression and function of Cx43 and Panx1 in rat and mouse nodose-petrosal-jugular complexes (NPJcs) using confocal immunofluorescence, molecular and electrophysiological techniques. Cx43 and Panx1 were detected in SGCs and in sensory neurons, respectively. In the rat and mouse, the electrical activity of vagal nerve increased significantly after nodose neurons were exposed to a Ca2+/Mg2+-free solution, a condition that increases the open probability of Cx hemichannels. This response was partially mimicked by a cell-permeable peptide corresponding to the last 10 amino acids of Cx43 (TAT-Cx43CT). Enhanced neuronal activity was reduced by Cx hemichannel, Panx1 channel and P2X7 receptor blockers. Moreover, the role of Panx1 was confirmed in NPJc, because in those from Panx1 knockout mice showed a reduced increase of neuronal activity induced by Ca2+/Mg2+-free extracellular conditions. The data suggest that Cx hemichannels and Panx channels serve as paracrine communication pathways between SGCs and neurons by modulating the excitability of sensory neurons.

show abstract

“…22) Using Tie-Cre recombinase and renin-Cre recombinase mice, Wagner et al reported that endothelial Cx40 is not essential for the control of systemic blood pressure and renin expression. 23) Thus, clarifying the molecular and subcellular mechanisms of Cx-mediated renal microcirculation in the pathophysiological settings awaits future studies, using refined methods of genetic engineering.…”

Section: Potential Roles Of Mesangial Cell–intercellular Communicatiomentioning

confidence: 99%

Exploring the mechanisms of renoprotection against progressive glomerulosclerosis

Oite

2011

Proceedings of the Japan Academy. Ser. B: Physical and Biologic

Self Cite

View full text Add to dashboard Cite

In this review, I introduce the strategy developed by our laboratory to explore the mechanisms of renoprotection against progressive glomerulosclerosis leading to renal death. First, I describe the experimental rat model in which disturbances of vascular regeneration and glomerular hemodynamics lead to irreversible glomerulosclerosis. Second, I discuss the possible mechanisms determining the progression of glomerulosclerosis and introduce a new imaging system based on intravital confocal laser scanning microscopy. Third, I provide an in-depth review of the regulatory glomerular hemodynamics at the cellular and molecular levels while focusing on the pivotal role of Ca2+-dependent gap junctional intercellular communication in coordinating the behavior of mesangial cells. Last, I show that local delivery of renoprotective agents, in combination with diagnostic imaging of the renal microvasculature, allows the evaluation of the therapeutic effects of angiotensin II receptor and cyclooxygenase activity local blockade on the progression of glomerulosclerosis, which would otherwise lead to renal death.

show abstract

Effects of connexin-mimetic peptides on perfusion pressure in response to phenylephrine in isolated, perfused rat kidneys

Abstract: This study showed that Cx43 plays a pivotal role in regulating renal vascular resistance and that Cx40 attenuates PE-induced vasoconstriction. These results provide new evidence that gap junctions may control renal circulation and vascular responses.

Cited by 9 publications

References 28 publications

Functional roles of connexins and pannexins in the kidney

Functional roles of connexins and pannexins in the kidney

Opening of pannexin- and connexin-based channels increases the excitability of nodose ganglion sensory neurons

Exploring the mechanisms of renoprotection against progressive glomerulosclerosis

Contact Info

Product

Resources

About