Effects of continuous activation of vitamin D and Wnt response pathways on osteoblastic proliferation and differentiation

Shi, Yan‐Chuan; Worton, Leah E.; Esteban, Luis; Baldock, Paul A.; Fong, Colette; Eisman, John A.; Gardiner, Edith M.

doi:10.1016/j.bone.2007.04.174

Cited by 55 publications

(37 citation statements)

References 54 publications

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“…A species specific effect of 1,25D on mineralization was observed in past literature, where 1,25D supplementation resulted in increased mineralization in human osteoblast cultures [17,32,35,42,43], but had primarily negative effects on mineralization in the mouse MC3T3 cell line [25,44]. In addition, the supplementation of 1,25D to mice resulted in increased levels of pyrophosphate and therefore decreased mineralization of bones within the mouse [45], as there needs to be low pyrophosphate levels for mineralization to occur in vitro and within the body [46].…”

Section: Figurementioning

confidence: 56%

Effects of Vitamin D, K1, and K2 Supplementation on Bone Formation by Osteoblasts In Vitro: A Meta-analysis

Lancaster¹,

Harrison²

2017

J Biom Biostat

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Bone loss is a major health problem that many aging individuals will face and thus research focusing on enhancing bone formation is of great importance. Cell biology or in vitro studies are particularly useful in exploring the exact effects a vitamin, supplement or drug has on particular processes within a certain cell type. Although there have been many cell biology articles focusing on the effects of vitamin D, K 1 or K 2 addition on bone formation in vitro, there has yet to be a consensus amongst the literature. The purpose of this article is to determine the effects of vitamin D, K 1 and K 2 supplementation on osteoblast maturation parameters through meta-analysis of past cell biology literature. A Hedges d effect size was calculated for each experiment extracted from past literature and the experiments were grouped by experiment and cell type. Homogeneity was assessed by the Cochran's Q test, while the effect sizes' departure from zero was assessed by a 95% confidence interval and a non-directional test. Supplementation with vitamin D, K 1 and K 2 , along with the combination of vitamin K 2 + 1,25-dihydroxyvitamin D, increased bone mineralization, while not consistently affecting all of the other parameters associated with bone formation. Vitamin K 2 and D addition had variable effects on bone formation using different cell types, which calls into question the suitability of particular cell lines as models for clinical trials. Therefore, the conditions and parameters in which bone formation is studied in vitro must be considered carefully before running a vitamin supplementation or drug-testing experiment.

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Section: Figurementioning

confidence: 56%

Effects of Vitamin D, K1, and K2 Supplementation on Bone Formation by Osteoblasts In Vitro: A Meta-analysis

Lancaster¹,

Harrison²

2017

J Biom Biostat

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“…NICD then translocates into the nucleus where it interacts with the transcription factor CCAAT-binding factor 1 (CBF-1) to activate transcription of several essential Notch target genes including hairy/enhancer of split 1 (HES1) and hairy/enhancer-of-split related with YRPW motif 1 (HEY1) (Weinmaster, 1997;Lai, 2002;Ehebauer et al, 2006). It has been demonstrated that HES1 and HEY1 regulate osteoblast differentiation through the modulation of Runx2 and osteocalcin expression (Zamurovic et al, 2004;Zhang et al, 2009).This purported role of Notch signaling in osteoblast differentiation, however, is somewhat controversial, as a number of previously published studies argue both for and against the hypothesis (Tezuka et al, 2002;Sciaudone et al, 2003;Zamurovic et al, 2004;Deregowski et al, 2006;Hilton et al, 2008;Zanotti et al, 2008;Zhang et al, 2009).To complicate matters, previous studies have also implicated the Wnt/b-catenin signaling pathway in the regulation of osteoblast differentiation (Gong et al, 2001;Kato et al, 2002;de Boer et al, 2004;Bodine and Komm, 2006;Shi et al, 2007). …”

mentioning

confidence: 92%

Porphyromonas gingivalis lipopolysaccharide inhibits the osteoblastic differentiation of preosteoblasts by activating Notch1 signaling

Xing

Fan

et al. 2010

Journal Cellular Physiology

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Although Porphyromonas gingivalis lipopolysaccharide (P-LPS) is known to inhibit osteoblast differentiation, the exact molecular mechanisms underlying this phenomenon remain unclear. Here, we investigated the role of Notch signaling in the osteoblastic differentiation of both MC3T3E-1 cells and primary mouse bone marrow stromal cells (BMSCs). P-LPS stimulation activated the Notch1 signaling cascade and increased expression of the Notch target genes HES1 and HEY1. P-LPS can also act as an inhibitor because it is capable of suppressing Wnt/beta-catenin signaling in preosteoblasts by decreasing both glycogen synthase kinase-3beta (GSK-3beta) phosphorylation and the expression of nuclear beta-catenin. These effects were rescued, however, by inhibiting Notch1 signaling. Furthermore, P-LPS treatment inhibited osteoblast differentiation in preosteoblasts as demonstrated by reductions in alkaline phosphatase activity, osteoblast gene expression, and mineralization, all of which were rescued by suppression of Notch1 signaling. Moreover, inhibition of GSK-3beta, HES1, or HEY1 partially reversed the P-LPS-induced inhibition of osteoblast differentiation. Together, these findings suggest that P-LPS inhibits osteoblast differentiation by promoting the expression of Notch target genes and suppressing canonical Wnt/beta-catenin signaling.

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“…Osteocalcin production indicates cell cycle arrest during cell division [16,17]. Generally, cells that exit cell cycle do not proliferate.…”

Section: Discussionmentioning

confidence: 99%

High porous titanium scaffolds showed higher compatibility than lower porous beta-tricalcium phosphate scaffolds for regulating human osteoblast and osteoclast differentiation

Hirota

Hayakawa

Shima

et al. 2015

Materials Science and Engineering: C

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Effects of continuous activation of vitamin D and Wnt response pathways on osteoblastic proliferation and differentiation

Cited by 55 publications

References 54 publications

Effects of Vitamin D, K1, and K2 Supplementation on Bone Formation by Osteoblasts In Vitro: A Meta-analysis

Effects of Vitamin D, K1, and K2 Supplementation on Bone Formation by Osteoblasts In Vitro: A Meta-analysis

Porphyromonas gingivalis lipopolysaccharide inhibits the osteoblastic differentiation of preosteoblasts by activating Notch1 signaling

High porous titanium scaffolds showed higher compatibility than lower porous beta-tricalcium phosphate scaffolds for regulating human osteoblast and osteoclast differentiation

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