Effects of copper-based compounds, antibiotics and a plant activator on population sizes and spread of Clavibacter michiganensis subsp. michiganensis in greenhouse tomato seedlings

Milijašević, Svetlana; Todorović, Biljana; Potočnik, Ivana; Rekanović, Emil; Stepanović, Miloš

doi:10.2298/pif0901019m

Cited by 12 publications

(8 citation statements)

References 27 publications

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“…() determined the in vitro sensitivity of Cmm strains to streptomycin, reporting an MIC value of 6 μg mL −1 . The effect of streptomycin on tomato plants has been evaluated in Spain (de León et al ., ), Serbia (Milijasevic et al ., ) and the USA (Hausbeck et al ., ; Werner et al ., ) under greenhouse conditions. In these studies, streptomycin applications were shown to be effective, reducing the symptoms of bacterial canker.…”

Section: Discussionmentioning

confidence: 99%

Streptomycin resistance in Clavibacter michiganensis subsp. michiganensis strains from Chile is related to an rpsL gene mutation

et al. 2018

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Streptomycin has been used for decades in Chile to control Clavibacter michiganensis subsp. michiganensis (Cmm), the causal agent of tomato bacterial canker. The aim of this work was to evaluate streptomycin resistance and to analyse the presence of resistance‐related genes in Cmm strains from Chile. A collection of 25 Cmm strains isolated from different localities in central Chile between 1996 and 2015 was analysed. Minimum inhibitory concentration (MIC) of streptomycin was determined. A search of streptomycin resistance‐related genes was carried out in Cmm genomes, and the presence of these genes was studied in all Chilean strains using PCR and sequencing techniques. MIC results showed that four of 25 strains were highly sensitive to streptomycin, with MIC values <2 μg mL−1. The remaining 21 strains possessed MIC of streptomycin ≥100 μg mL−1. The strB gene, encoding an aminoglycoside 6‐phosphotransferase that inactivates streptomycin, was detected in all Chilean strains, including sensitive and resistant strains. In the 21 resistant strains, a mutation in codon 43 of the rpsL gene was determined, conferring high streptomycin resistance. Interestingly, the four streptomycin‐sensitive Cmm strains did not possess this mutation. This study proposes that the continuous use of streptomycin leads to emergence of resistant Cmm strains, challenging researchers to look for novel alternatives to control this plant pathogenic bacterium.

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Section: Discussionmentioning

confidence: 99%

Streptomycin resistance in Clavibacter michiganensis subsp. michiganensis strains from Chile is related to an rpsL gene mutation

et al. 2018

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“…stewartii yang terdapat dalam benih walaupun belum mampu mengeliminasinya. Milijašević et al (2009) melaporkan bahwa streptomisin sulfat dengan konsentrasi 0.025% mampu mengurangi populasi patogen Clavibacter michiganensis subsp. michiganensis pada persemaian tomat di rumah kaca.…”

Section: Percobaan I: Penentuan Treatment Window Perlakuan Panas Keriunclassified

Perlakuan Panas Kering dan Bakterisida untuk Menekan Infeksi Pantoea stewartii subsp. stewartii pada Benih Jagung Manis

Nalis

Suastika

Giyanto

2015

J Fitopatol Indones

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Penyakit layu Stewart merupakan penyakit penting pada tanaman jagung, khususnya jagung manis. Penyakit tersebut disebabkan oleh bakteri Pantoea stewartii subsp. stewartii (sinonim Erwinia stewartii) dan bersifat tular benih. Salah satu cara pengendalian penyakit ini ialah dengan perlakuan benih. Penelitian bertujuan menentukan keefektifan perlakuan panas kering, bakterisida, dan kombinasinya untuk mengeliminasi bakteri P. stewartii subsp. stewartii pada benih jagung manis tanpa merusak kualitas benih. Penelitian terdiri atas 3 tahap percobaan. Percobaan I dilakukan untuk menentukan treatment window perlakuan panas kering dan bakterisida yang dilakukan pada benih jagung manis dan bakteri P. stewartii subsp. stewartii pada kondisi in vitro. Percobaan II merupakan perlakuan panas kering atau bakterisida pada benih jagung manis yang terinfeksi P. stewartii subsp. stewartii. Percobaan III merupakan kombinasi perlakuan panas kering dan bakterisida pada benih yang terinfeksi P. stewartii subsp. stewartii. Hasil penelitian menunjukkan bahwa perlakuan panas kering pada suhu 50 °C selama 24 jam mampu mematikan P. stewartii subsp. stewartii secara in vitro, namun tidak efektif pada benih yang terinfeksi (secara in vivo). Perlakuan panas kering pada benih sampai suhu 55 °C tidak menurunkan persentase daya berkecambah benih. Perlakuan bakterisida pada konsentrasi 100 ppm dapat mengurangi populasi bakteri yang terdapat dalam benih. Konsentrasi bakterisida 150 dan 200 ppm dapat secara nyata menurunkan populasi bakteri yang terdapat dalam benih, namun bersifat fitotoksik ke tanaman. Kombinasi perlakuan bakterisida (100 ppm) sebelum perlakuan panas kering (55 °C selama 24 jam) mampu mengeliminasi bakteri dalam benih dengan persentase daya kecambah di atas 85%.

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“…tomato, which continue evolving (Milijasevic et al, 2009a). The use of antibiotics has also been considered as ineffective against several pathogens and many countries do not permit its use.…”

Section: Introductionmentioning

confidence: 99%

Essential oils for the control of bacterial speck in tomato crop

Silva¹,

Martins²,

Alves³

2014

Afr. J. Agric. Res.

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Bacterial speck (Pseudomonas syringae pv. tomato) is considered one of the major diseases of tomato crop worldwide and alternative methods to control it are desirable. The objective of this study was to evaluate the effect of essential oils (EOs) on P. syringae pv. tomato strain ufv-1 growth, in controlling bacterial speck in tomato plants, as well as to find the best application time of the EOs. The EOs used in this study were thyme (TH), eucalyptus (EU), tea tree (TT), clove (CL), cinnamon (CN), citronella (CI), and lemon grass (LE). An in vitro test using EOs were conducted to verify the ufv-1 inhibition, and two tests were carried out in a greenhouse to evaluate the effect of EOs before and after inoculation with Pst. Inhibition zones were observed for EU, CI, CL, and CN at a concentration of 1%. Plants pre-treated with EOs showed lower disease severity than that in plants post-treated with EOs (P<0.05), whereas higher efficacy was observed using acibenzolar-S-methyl and CI (91 and 83%, respectively). Regarding post-treatment, TT, TH, CI, EU, LE, and commercial fungicide, resulted in reducing disease severity by 8 to 40% compared to control (water). Results from this study showed the potential use of EOs in controlling bacterial speck in tomato and suggest the induced resistance as the major mode of action.

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Effects of copper-based compounds, antibiotics and a plant activator on population sizes and spread of Clavibacter michiganensis subsp. michiganensis in greenhouse tomato seedlings

Cited by 12 publications

References 27 publications

Streptomycin resistance in Clavibacter michiganensis subsp. michiganensis strains from Chile is related to an rpsL gene mutation

Streptomycin resistance in Clavibacter michiganensis subsp. michiganensis strains from Chile is related to an rpsL gene mutation

Perlakuan Panas Kering dan Bakterisida untuk Menekan Infeksi Pantoea stewartii subsp. stewartii pada Benih Jagung Manis

Essential oils for the control of bacterial speck in tomato crop

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