Effects of copper on gill structure and transport function in the rainbow trout, <i>oncorhynchus mykiss</i>

Sola, F. Gil de; Isaia, J.; Masoni, A.

doi:10.1002/jat.2550150509

Cited by 62 publications

(19 citation statements)

References 29 publications

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“…Some studies revealed that interstitial edema is one of the more frequent lesions observed in gill epithelium of fish exposed to heavy metals (Mallatt 1985). The results of this study confirm the occurrence of edema independently of copper levels, as in other fish species (Sola et al 1995, Bury et al 1998. The lifting of lamellar epithelium is other histological change observed, probably induced by the incidence of severe edema (Arellano et al 1999, Pane et al 2004, Schwaiger et al 2004.…”

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confidence: 85%

Histopathological changes in liver and gill epithelium of Nile tilapia, Oreochromis niloticus, exposed to waterborne copper

et al. 2007

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Nile tilapia, Oreochromis niloticus, of both sexes were reared in freshwater and exposed to 0.5, 1.0 and 2.5mg L-1 of waterborne copper for a period of 21 days. Liver and gill samples were collected after 21 days of exposure to copper and lesions were analyzed by light microscopy. The main histopathological changes observed in gills exposed to the highest concentration were edema, lifting of lamellar epithelia and an intense vasodilatation of the lamellar vascular axis. Although less frequent, lamellar fusion caused by the filamentar epithelium proliferation and some lamellar aneurisms were also found. The liver of control group exhibited a quite normal architecture, while the fish exposed to copper showed vacuolation and necrosis. These hepatic alterations were more evident in fish exposed to 1.0 and 2.5mg L-1 copper concentrations. The number of hepatocytes nucleus per mm² of hepatic tissue decreased with the increase of copper concentration. In contrast, the hepatic somatic index was high in fish exposed at 2.5mg L-1 of copper. In short, this work advance new knowledge as influence of copper in the gill and liver histology of O. niloticus and demonstrated that their effects could be observed at different concentrations.

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Section: Discus Discus Discus Discus Discussion Sion Sion Sion Sionsupporting

confidence: 85%

Histopathological changes in liver and gill epithelium of Nile tilapia, Oreochromis niloticus, exposed to waterborne copper

et al. 2007

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“…Lamellar epithelial lifting, hyperplasia, and lamellar fusion are nonspecific responses that are known to be induced by many gill tissue irritants (Mallatt, 1985). However, focal points of cellular hypertrophy and necrosis followed by epithelial rupture reflect the direct deleterious effects of heavy metals in fish gills (Mallatt, 1985;Sola et al, 1995). All these lesions may impair respiratory function.…”

Section: Discussionmentioning

confidence: 99%

Toxicity of copper in butterfish (Poronotus triacanthus): Tissues accumulation and ultrastructural changes

Jiraungkoorskul

Sahaphong

Kangwanrangsan

2007

Environmental Toxicology

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The present study investigated the accumulation of copper in various tissues and ultrastructural alterations in butterfish, Poronotus triacanthus. In acute toxicity test, butterfish were exposed to 250 g/L copper for 24 h. In subacute toxicity test, fish were exposed to 25 g/L copper for 7 days and then returned to normal water for 48 h. The levels of copper accumulation in the tissues were determined by using an atomic absorption spectrometer. After the 7 day exposure, the highest level of copper was found in the liver, followed by kidney, gills, and muscle tissues (3.64, 0.62, 0.59, and 0.34 g/L, respectively). The recovery group has shown some reduction in copper level in these tissues when compared with those of the 7 day exposure group (3.09, 0.34, 0.30, and 0.27 g/L, respectively). In gills, the major changes such as filament cell proliferation, increase in intercellular spaces, epithelial lifting, and thickening of the filament and lamellar epithelium were observed. In liver, the major changes such as swollen mitochondria, fragmented in rough endoplasmic reticulum, increases in number and size of lysosomes and lipid droplets. Infiltration of leukocytes, increasing hepatocyte size with pyknotic nuclei, and presence of vacuoles were also observed. In kidney, the changes included alterations of the first proximal tubules, as well as vacuolization of the cytoplasm, proliferation of lysosomes and mitochondria, dilation of endoplasmic reticulum, and finally, cell necrosis. The transmission electron microscopic analysis revealed that the 7 day exposure group had more severe effect in tissue alterations than the 24 h exposure group. Tissue regeneration was also observed in the 48 h recovery group. # 2007 Wiley Periodicals, Inc. Environ Toxicol 22: 92-100, 2007.

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“…One of the ways that waterborne Cu causes ionoregulatory disturbances in fish is via non-competitive inhibition of branchial Na + /K + -ATPase activity (Laurén and McDonald, 1987a;Sola et al, 1995;Pelgrom et al, 1995;Li et al, 1998). Intracellular Cu competes with Mg 2+ for binding sites on the ATP molecule and forms a physiologically inert ATP complex, Cu-ATP (Li et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Dietary sodium inhibits aqueous copper uptake in rainbow trout(Oncorhynchus mykiss)

Pyle

Kamunde

McDonald

et al. 2003

Journal of Experimental Biology

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SUMMARYOurs is the first study to demonstrate an influence of dietary sodium on waterborne copper uptake in fish. We examined possible interactions between dietary sodium and the response of freshwater rainbow trout (Oncorhynchus mykiss) to waterborne copper in light of recent evidence of interactions between sodium and copper metabolism in the gills. Trout were maintained for 6 days on one of four diets of increasing sodium concentration (0.25 mmol g-1, 0.51 mmol g-1, 0.76 mmol g-1 and 1.27 mmol g-1, which corresponds to 0.6%, 1.2%, 1.8% and 3% sodium by mass, respectively). At the end of 7 days, fish were exposed for 6 h to waterborne copper spiked with 64Cu to determine if the dietary sodium affected responses to a subsequent short-term waterborne copper exposure. The radiotracer allowed us to distinguish between Cu occurring in fish tissues before the experiment and `newly accumulated' Cu arising from the experimental exposure. Dietary sodium concentrations of 1.8% or 3% reduced newly accumulated copper concentrations in gill (from 93.9 ng g-1in control to 38.9 ng g-1 and 20.0 ng g-1 in fish fed 1.8% or 3% Na+-supplemented diets, respectively), liver (from 64.3 ng g-1 to 23.1 ng g-1 and 7.5 ng g-1,respectively), kidney (from 29.3 ng g-1 to 11.7 ng g-1and 7.8 ng g-1, respectively), plasma (from 64.7 ng g-1to 21.5 ng g-1 and 10.7 ng g-1, respectively) and gut(from 6.8 ng g-1 to 3.4 ng g-1 and 2.2 ng g-1, respectively) by 50.0-88.2%. The 3%Na+-supplemented diets also increased plasma and gut sodium concentrations by 38.1% (from 137.1 μmol g-1 to 189.3 μmol g-1) and 104.3% (from 56.5 μmol g-1 to 115.4 μmol g-1), respectively, relative to fish maintained on untreated diets. Whole body uptake rates of both sodium and copper were significantly reduced,and highly correlated (r=0.97) with one another, in fish fed high-sodium diets relative to controls. Moreover, sodium efflux was 12% and 38% higher in fish fed 1.8% and 3% sodium-enriched diets, respectively. Fish fed high-sodium diets also drank more water, but the contribution of drinking to waterborne copper uptake was negligible. From these results, we speculate that, at least in part, aqueous sodium and copper share a common branchial uptake route, probably through an apical sodium channel. According to this hypothesis, as the channel is downregulated with increasing internal sodium concentrations, both sodium and copper uptake from the water are inhibited.

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Effects of copper on gill structure and transport function in the rainbow trout, oncorhynchus mykiss

Cited by 62 publications

References 29 publications

Histopathological changes in liver and gill epithelium of Nile tilapia, Oreochromis niloticus, exposed to waterborne copper

Histopathological changes in liver and gill epithelium of Nile tilapia, Oreochromis niloticus, exposed to waterborne copper

Toxicity of copper in butterfish (Poronotus triacanthus): Tissues accumulation and ultrastructural changes

Dietary sodium inhibits aqueous copper uptake in rainbow trout(Oncorhynchus mykiss)

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