Effects of copper sulphate and cobalt chloride on &lt;i&gt;in vitro&lt;/i&gt; performances of traditional indica rice (&lt;i&gt;Oryza sativa&lt;/i&gt; L.) varieties in Sri Lanka

Amarasinghe, A. A. Y.

doi:10.4038/jas.v4i3.1652

Cited by 8 publications

(4 citation statements)

References 14 publications

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“…Increase in MDA content following Co 2+ treatment was also recorded in Ocimum basilicum plants (20). In harmony with our results, Amarasinghe (2009) recorded decrease in rate of callus proliferation in four rice cultivars growing on media containing 10 mg/L of cobalt chloride. In the same context, Javed and Anis (2015) demonstrated decrease in shoot length of Erythrina variegate in vitro plants at 100 and 150 µM of cobalt chloride.…”

Section: Discussionsupporting

confidence: 89%

Effect of cobalt nanoparticles and cobalt ions on alkaloids production and expression of CrMPK3 gene in Catharanthus roseus suspension cultures

Fouad

Hafez

2018

Cell Mol Biol (Noisy-le-grand)

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The capabilities of cobalt ions (Co 2+) and cobalt nanoparticles (CoNPs) in enhancing alkaloids accumulation in Catharanthus roseus suspension cultures were evaluated in relation to the expression of CrMPK3 gene. Four concentrations (5, 10, 15 and 20 mg/L) of each cobalt form were utilized in addition to control. Both forms induced oxidative stress that was more pronounced for Co 2+ treatments. A positive correlation was observed between cobalt concentration and expression of CrMPK3 gene. However, a characteristic temporal expression profile was recorded for each cobalt form. Also, positive correlations were detected between both cobalt concentration and expression of CrMPK3 gene on one hand and the activities of antioxidant enzymes (SOD and APX) and alkaloids content on the other hand. Such correlations suggest CrMPK3 gene as a common player in cobalt-induced stress signaling; regardless of cobalt form. Results revealed the higher capability of Co 2+ , compared with CoNPs, in enhancing alkaloids accumulation. However, results supported CoNPs as a novel tool in manipulating cobaltinduced alkaloids production in C. roseus.

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Section: Discussionsupporting

confidence: 89%

Effect of cobalt nanoparticles and cobalt ions on alkaloids production and expression of CrMPK3 gene in Catharanthus roseus suspension cultures

Fouad

Hafez

2018

Cell Mol Biol (Noisy-le-grand)

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“…Tissue culture techniques have become necessary for the production of the transgenic rice plants (Peng et al 1992), hybrids (Mariam et al 1996), and for the recovery of germplasm when seed availability is limited. These methods are based on adventitious shoot culture (Shahsavari 2011) and somatic embryogenesis (Amarasinghe 2009) which resulted into genetic variations in rice cultivars (Mannan et al 2013). Micropropagation protocols have been established for japonica and indica rice cultivars using as explants like as mature seeds (Ullah et al 2007), shoot segments (Verma et al 2011), embryos (Ali et al 2004; Evangelista et al 2009), anthers (Khatun et al 2010) which produced regenerated plantlets.…”

Section: Introductionmentioning

confidence: 99%

Optimization of regeneration using differential growth regulators in indica rice cultivars

Sankepally

Singh

2016

3 Biotech

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Paddy is a staple crop and being grown in major parts of India, but similarly it is facing some major challenges like abiotic stresses (drought, salinity, etc.) by which its productivity is greatly affected. De-husked seeds of 21 cultivars of indica rice were cultured onto MS medium for screening of their micropropagation capabilities. All the selected cultivars are being cultivated by farmers of Andhra Pradesh and Rajasthan states of India, these cultivars bear good quality of grain and high yield. Similarly, both states are facing the problem of drought so; we selected these cultivars for the micropropagation and transformation studies. The induction of callus and regeneration in these selected cultivars were achieved on MS culture medium with supplementation of various growth regulators. Maximum callus induction and regeneration frequency were observed in Sambha mahsuri at the concentration of 3.0 mg L−1 of 2,4-D and BAP (5.0 mg L−1) while the moderate response achieved in other cultivars of rice. Sambha mahsuri showed promising results in terms of callus induction frequency, and regeneration potentialities of embryogenic callus so, there is need to develop a rice cultivars that could thrive under drought condition if the country is to sustain rice production. Finally, regenerated plantlets were successfully transferred to natural conditions (in pots) after acclimatization.

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“…However, Cu is too toxic at high concentrations causing a reduced photosynthetic rate, increasing the formation of reactive oxygen species (ROS), causing rising leakage of potassium ions from the roots [ 10 ], and inducing the deficiency of iron [ 5 ]. Many reports have indicated the positive effects of higher Cu concentrations on in vitro culture of many plants, such as barley [ 11 ], tobacco [ 12 ], Lepidium sativum [ 13 ], rice [ 14 ], carrot [ 15 ] and Gymnema sylvestre [ 16 ], as reported by [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 95%

In Vitro Propagation and Acclimatization of Banana Plants: Antioxidant Enzymes, Chemical Assessments and Genetic Stability of Regenerates as a Response to Copper Sulphate

Elyazid

Salama

Zanaty

et al. 2021

Plants

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Developing a successful protocol for banana in vitro culture is a guarantee for the mass propagation of pathogen-free, high-quality, true-to-type planting materials with low production costs. The current work aimed to investigate the influence of increasing copper levels in an MS medium on endophytic bacterial contamination; shoot multiplication; rooting and the acclimatization of in vitro cultured banana; minerals and chlorophyll content; antioxidant enzymes activity; electrolyte leakage; and the genetic stability of banana regenerants. Four different concentrations of copper sulphate (0.025 as a control, and 30, 60, and 120 mg L−1) were examined. The growth of the endophytic bacteria was inhibited at 60 mg L−1 of copper sulphate which recorded zero contamination, without a significant difference at 120 mg L−1. However, 0.025 mg L−1 of copper sulphate was optimal for the maximum shoot number and shoot length (10 shoots and 6 cm, respectively) without significant differences at 30 mg L−1. The root length of banana plantlets was significantly enhanced at 30 mg L−1 of copper sulphate but without significant differences to the control, regarding the number of roots (9.92 cm and 3.80 roots, respectively). In vitro plants were acclimatized successfully at 30 mg L−1 of copper sulphate with 100% survival. The uptake of minerals, antioxidant enzyme activity and electrolyte leakage was improved because of the copper sulphate, but the chlorophyll level decreased. RAPD profiling showed polymorphism in only one plant treated with 60 mg L−1 of copper sulphate, with an average of 1.8%. The genome template stability percentage was almost 100% for all treated plants.

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Effects of copper sulphate and cobalt chloride on <i>in vitro</i> performances of traditional indica rice (<i>Oryza sativa</i> L.) varieties in Sri Lanka

Abstract: ABSTRACT

Cited by 8 publications

References 14 publications

Effect of cobalt nanoparticles and cobalt ions on alkaloids production and expression of CrMPK3 gene in Catharanthus roseus suspension cultures

Effect of cobalt nanoparticles and cobalt ions on alkaloids production and expression of CrMPK3 gene in Catharanthus roseus suspension cultures

Optimization of regeneration using differential growth regulators in indica rice cultivars

In Vitro Propagation and Acclimatization of Banana Plants: Antioxidant Enzymes, Chemical Assessments and Genetic Stability of Regenerates as a Response to Copper Sulphate

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