In Vitro Propagation and Acclimatization of Banana Plants: Antioxidant Enzymes, Chemical Assessments and Genetic Stability of Regenerates as a Response to Copper Sulphate

Elyazid, Doaa M. Abou; Salama, A. S.; Zanaty, Abdel Fattah M. El; Abdalla, Neama

doi:10.3390/plants10091853

Cited by 10 publications

(8 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The photosynthetic pigments (Chl a, Chl b, and carotenoids) of banana transplants during the acclimatization stage are crucial biological components because their efficiency may guarantee high survivorship of banana transplants. These components can support banana transplants with enough photosynthetic products, which strengthen the vegetative growth of transplants [38]. The applied nanofertilizers of Cu or Se increased the banana leaves' content of photosynthetic pigments up to 100 mg L −1 of nano-Cu or nano-Se.…”

Section: Photosynthetic Pigments and Fluorescencementioning

confidence: 99%

Acclimatization of In Vitro Banana Seedlings Using Root-Applied Bio-Nanofertilizer of Copper and Selenium

et al. 2022

View full text Add to dashboard Cite

The production of in vitro banana transplants has become an important practice in the global banana production. Proper and enough nutrients are needed for banana production particularly during the acclimatization period. To avoid the environmental problem resulting from the chemical fertilizers, nanofertilizers of Se and Cu were separately applied during the acclimatization of banana. The biological form of nano-Cu (50 and 100 mg L−1) and nano-Se (25, 50, 75, and 100 mg L−1) were studied on acclimatized banana transplants under greenhouse conditions. Both applied nanofertilizers enhanced the growth of transplant by 10.9 and 12.6% for dry weight after nano-Se and nano-Cu application up to 100 mg L−1, respectively. The survival rate was also increased by increasing applied doses of both nanofertilizers up to 100 mg L−1, whereas the highest survival rate (95.3%) was recorded for nano-Cu. All studied photosynthetic pigments and its fluorescence were improved by applying nanofertilizers. Studied antioxidant enzymatic activities (CAT, PPO, and POX) were also increased. A pH decrease in the growing medium was noticed after applying nano-Cu, which may explain the high bioavailability of studied nutrients (N, P, K, Cu, Fe, Se, and Zn) by banana transplants.

show abstract

Section: Photosynthetic Pigments and Fluorescencementioning

confidence: 99%

Acclimatization of In Vitro Banana Seedlings Using Root-Applied Bio-Nanofertilizer of Copper and Selenium

et al. 2022

View full text Add to dashboard Cite

show abstract

“…For Corylus avellana L., Akin et al (2017) tested the effect of Ca 2+ , Mg 2+ , K + , NH4 + , NO3and SO4 2ions and give the best concentrations, to be added in media, allowing the best callus formation. More recently, the study of Elyazid et al (2021) optimized the concentration of CuSO4 in banana tissue culture media to improve the growth and development of micropropagated banana plants by enhancing shooting and rooting. A concentration of 0.025 mg/L of copper sulphate was optimal for the maximum shoot number and length.…”

Section: Effects Of Potassium and Sodium Ions On The Callogenesis Ind...mentioning

confidence: 99%

Induction of organogenesis and callogenesis in Limbarda crithmoïdes L. (Asteraceae) explants cultured on MS media supplemented with various concentrations of Na+ and K+

BOKRI

KAHLAOUI

Hcini

et al. 2023

Not Bot Horti Agrobo

View full text Add to dashboard Cite

The present work describes an efficient micropropagation and callus induction protocol of Limbarda crithmoïdes L. (Asteraceae), a halophyte species with medicinal and horticultural interests. The objective was to identify the culture media that support the best organogenesis and callogenic expressions of four types of aerial explants; nodal and internodal segments and leaf portions, by varying the mineral composition of twenty culture media by adding increasing concentrations of Na+ (from 0-100 mM) and K+ (from 0-50 mM). After two months of culture, parameters relating to the various expressions of organogenesis and callogenesis were measured. Results showed that the K+ and Na+ interactions affect the explants development. The combination of high concentrations of Na+ (50, 100 mM) and K+ (50 mM) ions allowed the most important regeneration of the axillary shoots (70.6-100%), root neoformation (82.6-96.1%) and callus induction (76.3-100%). Rooted plantlets with well-developed axillary shoots have been successfully acclimatized with a 93% of survival rate. The selected media would allow a large-scale multiplication of this medicinal species, without adding exogenous phytohormones, and could be used for the micropropagation of other threatened halophyte species or for the production of callus; rich in secondary metabolites.

show abstract

“…Endophytic microbes that are present within the explants are considered a major constrain to the establishment and growth of tissue-cultured plants, as they are more difficult to remove by normal surface sterilization. However, the internal bacterial contamination could be eliminated by supplying the culture media with different substances (Table 2), for example, antibiotics [44,45], copper sulfate [44,46], or fungicides [47]. After identification of the contaminants, a low-phytotoxicity antibiotic should be selected [47].…”

Section: Sargassum Fusiformementioning

confidence: 99%

An Academic and Technical Overview on Plant Micropropagation Challenges

et al. 2022

Self Cite

View full text Add to dashboard Cite

The production of micropropagated plants in plant-tissue-culture laboratories and nurseries is the most important method for propagation of many economic plants. Micropropagation based on tissue-culture technology involves large-scale propagation, as it allows multiplication of a huge number of true-to-type propagules in a very short time and in a very limited space, as well as all year round, regardless of the climate. However, applying plant-tissue-culture techniques for the commercial propagation of plants may face a lot of obstacles or troubles that could result from technical, biological, physiological, and/or genetical reasons, or due to overproduction or the lack of facilities and professional technicians, as shown in the current study. Moreover, several disorders and abnormalities are discussed in the present review. This study aims to show the most serious problems and obstacles of plant micropropagation, and their solutions from both scientific and technical sides. This review, as a first report, includes different challenges in plant micropropagation (i.e., contamination, delay of subculture, burned plantlets, browning, in vitro rooting difficulty, somaclonal variations, hyperhydricity, shoot tip necrosis, albino plantlets, recalcitrance, shoot abnormalities, in vitro habituation) in one paper. Most of these problems are related to scientific and/or technical reasons, and they could be avoided by following the micropropagation protocol suitable for each plant species. The others are dominant in plant-tissue-culture laboratories, in which facilities are often incomplete, or due to poor infrastructure and scarce funds.

show abstract

In Vitro Propagation and Acclimatization of Banana Plants: Antioxidant Enzymes, Chemical Assessments and Genetic Stability of Regenerates as a Response to Copper Sulphate

Cited by 10 publications

References 51 publications

Acclimatization of In Vitro Banana Seedlings Using Root-Applied Bio-Nanofertilizer of Copper and Selenium

Acclimatization of In Vitro Banana Seedlings Using Root-Applied Bio-Nanofertilizer of Copper and Selenium

Induction of organogenesis and callogenesis in Limbarda crithmoïdes L. (Asteraceae) explants cultured on MS media supplemented with various concentrations of Na+ and K+

An Academic and Technical Overview on Plant Micropropagation Challenges

Contact Info

Product

Resources

About