Effects of Crude Oil, Dispersant, and Oil-Dispersant Mixtures on Human Fecal Microbiota in an
            <i>In Vitro</i>
            Culture System

Kim, Jong‐Nam; Kim, Bongsoo; Kim, Seong-Jae; Cerniglia, Carl E.

doi:10.1128/mbio.00376-12

Cited by 8 publications

(5 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is suggestive that hydrocarbon solubility varies between the dispersants and may directly impact bioavailability of these toxicants into the environment (Su, 2006; Wu et al, 2012). Given that WAF-9527 + oil induced DSB damage exhibited comparable levels to WAF-9527 only treatment, these findings corrobotate similar studies confirming differences in toxic response between dispersant only and dipsersed oil exposure (Hemmer, 2010; Kim et al, 2012). …”

Section: Discussionsupporting

confidence: 75%

Effects of corexit oil dispersants and the WAF of dispersed oil on DNA damage and repair in cultured human bronchial airway cells, BEAS-2B

et al. 2016

View full text Add to dashboard Cite

Large quantities of dispersants were used as a method to disperse the roughly 210 million gallons of spilled crude oil that consumed the Gulf of Mexico. Little is known if the oil-dispersant and oil-dispersant mixtures on human airway BEAS-2B epithelial cells. Here we present the cytotoxic and genotoxic in vitro effects on the human lung cells BEAS-2B following exposure to and oil-dispersant mixtures on human airway BEAS-2B epithelial cells. Here we present the cytotoxic and genotoxic in vitro effects on the human lung cells BEAS-2B following exposure to Corexit dispersants EC9500 and EC9527, Water Accommodated Fraction (WAF) -crude, WAF-9500 + Oil, and WAF-9527 + Oil. Cellular cytotoxicity to WAF-dispersed oil samples was observed at concentrations greater than 1000 ppm with over 70% of observed cellular death. At low concentration exposures (100 and 300 ppm) DNA damage was evidenced by the detection of single strand breaks (SSBs) and double strand breaks (DSBs) as measured by alkaline and neutral comet assay analyses. Immunoblot analyses of the phosphorylated histone H2A.X (ɣ-H2A.X) and tumor suppressor p53 protein confirmed activation of the DNA damage response due to the exposure-induced DNA breaks. Although, many xenobiotics interfere with DNA repair pathways, in vitro evaluation of the nucleotide excision repair (NER) and DSB repair pathways appear to be unaffected by the oil-dispersant mixtures tested. Overall, this study supports that oil-dispersant mixtures induce genotoxic effects in culture.

show abstract

Section: Discussionsupporting

confidence: 75%

Effects of corexit oil dispersants and the WAF of dispersed oil on DNA damage and repair in cultured human bronchial airway cells, BEAS-2B

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Pyrosequencing analysis was conducted as previously described (24,25). The taxonomic classification of reads was conducted using the EzTaxon-e database (26).…”

Section: Fecal Enzyme Activity Analysismentioning

confidence: 99%

Kimchi improves irritable bowel syndrome: results of a randomized, double-blind placebo-controlled study

Kim

Park²,

Choi

et al. 2022

Food & Nutrition Research

View full text Add to dashboard Cite

Background: Irritable bowel syndrome (IBS) can be caused by abnormal bowel movements, altered brain-gut axis, gut microbiota change, and low levels of inflammation or immune activation. The intake of food containing much fiber and lactic acid bacteria (LABs) can alleviate IBS. Objective: This study was undertaken to confirm the alleviative effect of kimchi on symptoms of IBS. Design: Three types of kimchi (standard kimchi, SK; dead nano-sized Lactobacillus plantarum nF1 (nLp) added to standard kimchi, nLpSK; or functional kimchi, FK) were given to 30 individuals in each of three groups, that is, the SK group (n = 30), the nLpSK group (n = 30), or the FK group (n = 30) at 210 g a day for 12 weeks. Food intake records, serum levels of inflammatory factors, fecal levels of harmful enzymes, and microbiome changes were investigated over the 12-week study period. Results: After intervention, dietary fiber intake was increased in all groups. Typical IBS symptoms (abdominal pain or inconvenience, desperation, incomplete evacuation, and bloating), defecation time, and stool type were also improved. In serum, all groups showed reductions in tumor necrosis factor (TNF)-α (P < 0.001) levels. In addition, serum IL-4 (P < 0.001), IL-10 (P < 0.001), and IL-12 (P < 0.01) were significantly reduced in the nLpSK and FK groups, and serum monocyte chemotactic protein (MCP)-1 (P < 0.05) was significantly reduced in the nLpSK group. Furthermore, activities of fecal β-glucosidase and β-glucuronidase were significantly decreased in all three groups, and these reductions were greatest in the nLpSK group. Gut microbiome analysis showed that kimchi consumption increased Firmicutes populations at the expense of Bacteroidetes and Tenericutes populations. In addition, the Bifidobacterium adolescentis population increased significantly in the FK group (P = 0.026). Conclusion: Kimchi intake helps alleviate IBS by increasing dietary fiber intake and reducing serum inflammatory cytokine levels and harmful fecal enzyme activities. Notably, nLp improved the immune system, and several functional ingredients in FK promoted the growth of Bifidobacterium adolescentis in gut.

show abstract

“…Laboratory experiments suggest that dispersant and dispersant-oil mixtures produce effects indicative of lung diseases such as asthma and chronic obstructive pulmonary disease (Liu et al, 2016) and mixtures may affect the gut microbiome (J.N. Kim et al, 2012).…”

Section: Health Effects On Non-workersmentioning

confidence: 99%

Human Health and Socioeconomic Effects of the Deepwater Horizon Oil Spill in the Gulf of Mexico

Sandifer¹,

Ferguson²,

Finucane³

et al. 2021

Oceanog

View full text Add to dashboard Cite

The Deepwater Horizon (DWH) oil spill is the only declared Spill of National Significance in US history, and it significantly impacted the health of people and communities in the Gulf of Mexico region. These impacts amplified adverse effects of prior disasters and may compound those of future traumas. Studies, both to date and ongoing, show some negative mental and physical health outcomes associated with DWH in some spill workers, as well as some coastal residents in all Gulf States. The spill was also associated with negative effects in the living resources, tourism, and recreation sectors, at least in the short term. Compared with others, people dependent on these sectors reported more health and financial concerns. Consumer concerns about the safety and marketability of seafood persisted well after data demonstrated very low risk. Parents were concerned about possible exposures of children as they played on beaches, but this risk was found to be minor. Spill-related stress was an overarching factor associated with adverse health outcomes, and some residents reported greater stress from navigating the legal and claims processes following the spill than from the spill itself. Research revealed a serious lack of baseline health, environmental, and socioeconomic data against which to compare spill effects. This finding highlighted the need for ongoing observing systems to monitor health and socioeconomic parameters and establish continuous baselines of such information.

show abstract

Effects of Crude Oil, Dispersant, and Oil-Dispersant Mixtures on Human Fecal Microbiota in an In Vitro Culture System

Cited by 8 publications

References 27 publications

Effects of corexit oil dispersants and the WAF of dispersed oil on DNA damage and repair in cultured human bronchial airway cells, BEAS-2B

Effects of corexit oil dispersants and the WAF of dispersed oil on DNA damage and repair in cultured human bronchial airway cells, BEAS-2B

Kimchi improves irritable bowel syndrome: results of a randomized, double-blind placebo-controlled study

Human Health and Socioeconomic Effects of the Deepwater Horizon Oil Spill in the Gulf of Mexico

Contact Info

Product

Resources

About