Effects of cryopreservation and coculture with pancreatic ductal epithelial cells on insulin secretion from human pancreatic islets

Gatto, Claudio; Callegari, Maura; Folin, Marcella; Conconi, Maria Teresa; Paolin, Adolfo; Falco, Giuseppe; Bredariol, Simonetta; Spinazzi, Raffaella; Parnigotto, Pier Paolo; Nussdorfer, Gastone G.

doi:10.3892/ijmm.12.6.851

Cited by 13 publications

(17 citation statements)

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“…Insulin secretion was expressed as absolute value, as percent of islet insulin content, and as stimulation index (SI), i.e. the ratio of stimulated over basal insulin secretion [35]. Insulin concentrations were measured by a commercially available immunoradiometric assay (Pantec Forniture Biomediche, Turin, Italy).…”

Section: Methodsmentioning

confidence: 99%

Functional and morphological alterations of mitochondria in pancreatic beta cells from type 2 diabetic patients

et al. 2005

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Aims/hypothesis: Little information is available on the insulin release properties of pancreatic islets isolated from type 2 diabetic subjects. Since mitochondria represent the site where important metabolites that regulate insulin secretion are generated, we studied insulin release as well as mitochondrial function and morphology directly in pancreatic islets isolated from type 2 diabetic patients. Methods: Islets were prepared by collagenase digestion and density gradient purification, and insulin secretion in response to glucose and arginine was assessed by the batch incubation method. Adenine nucleotides, mitochondrial membrane potential, the expression of UCP-2, complex I and complex V of the respiratory chain, and nitrotyrosine levels were evaluated and correlated with insulin secretion. Results: Compared to control islets, diabetic islets showed reduced insulin secretion in response to glucose, and this defect was associated with lower ATP levels, a lower ATP/ADP ratio and impaired hyperpolarization of the mitochondrial membrane. Increased protein expression of UCP-2, complex I and complex V of the respiratory chain, and a higher level of nitrotyrosine were also found in type 2 diabetic islets. Morphology studies showed that control and diabetic beta cells had a similar number of mitochondria; however, mitochondrial density volume was significantly higher in type 2 diabetic beta cells. Conclusions/ interpretation: In pancreatic beta cells from type 2 diabetic subjects, the impaired secretory response to glucose is associated with a marked alteration of mitochondrial function and morphology. In particular, UCP-2 expression is increased (probably due to a condition of fuel overload), which leads to lower ATP, decreased ATP/ADP ratio, with consequent reduction of insulin release.

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Section: Methodsmentioning

confidence: 99%

Functional and morphological alterations of mitochondria in pancreatic beta cells from type 2 diabetic patients

et al. 2005

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“…Pancreatic ductal epithelial cells have been considered as putative stem cells for islets and an essential component of the extracellular matrix, which plays an important role in secreting appropriate growth factors that support islet viability. Gatto et al (2003) found that long-term culture, as well as cryopreservation, decreased the viability of human pancreatic islets, which was prevented by coculture with ductal epithelial cells at 33°C. In a different study, coculture with ductal epithelial cells helped maintain structural integrity and viability of the islets (Ilieva et al, 1999).…”

Section: A Loss Of Islet Viability During Isolation and Culturementioning

confidence: 99%

“…Media composition, seeding density, and temperature play a significant role (Falqui et al, 1991;Murdoch et al, 2004). In addition, islet coculture with pancreatic ductal epithelial cells was shown to be useful for maintaining islet viability and function after isolation (Gatto et al, 2003). Pancreatic ductal epithelial cells have been considered as putative stem cells for islets and an essential component of the extracellular matrix, which plays an important role in secreting appropriate growth factors that support islet viability.…”

Section: A Loss Of Islet Viability During Isolation and Culturementioning

confidence: 99%

Biological and Biomaterial Approaches for Improved Islet Transplantation

2006

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“…Long-term culture as well as cryopreservation was shown to decrease the viability of human pancreatic islets, which was prevented by coculture with ductal epithelial cells (Gatto et al, 2003). It was also shown that after clinical islet transplantation, the insulin secretory response to glucose, a measure of the functional beta cell mass, correlates significantly with the number of duct cells originally present in the islets transplanted (Street et al, 2004).…”

Section: Pancreatic Progenitor Cellsmentioning

confidence: 84%

Pancreatic Islet Transplantation and Regeneration for Diabetes mellitus Treatment

Babarykin¹,

Nikolajeva²,

Eze³

et al. 2008

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

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Effects of cryopreservation and coculture with pancreatic ductal epithelial cells on insulin secretion from human pancreatic islets

Cited by 13 publications

References 0 publications

Functional and morphological alterations of mitochondria in pancreatic beta cells from type 2 diabetic patients

Functional and morphological alterations of mitochondria in pancreatic beta cells from type 2 diabetic patients

Biological and Biomaterial Approaches for Improved Islet Transplantation

Pancreatic Islet Transplantation and Regeneration for Diabetes mellitus Treatment

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