Effects of CSFS and their Combinations with Chemotherapeutic Agents (CH) on Leukemic Blasts (LB) in Children (MTT-Assay)

Литвинова, Е. Г.; Gurova, Katerina V.; Чимишкян, К. Л.; Менткевич, Г. Л.

doi:10.1007/978-1-4615-4811-9_65

Cited by 3 publications

(3 citation statements)

References 12 publications

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“…Cell proliferation was evaluated by the MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide] assay as described previously (15). The IC50 (half maximal inhibitory concentration) values were determined directly from the semilogarithmic dose-response curves.…”

Section: Mtt Analysismentioning

confidence: 99%

Comparative Mitochondrial Proteomic Analysis of Raji Cells Exposed to Adriamycin

Jiang

Sun

Fang

et al. 2009

Mol Med

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The antitumor mechanisms of adriamycin (ADR) have been thought to contribute to induction of apoptosis and inefficiency of DNA repair, processes that are to a large extent mediated by mitochondria. This study aimed to investigate characteristics of ADR, including its antineoplastic activity, drug resistance, and unexpected toxicity in non-Hodgkin lymphoma (NHL) Raji cells at the mitochondrial proteomic level. The alterations of the mitochondrial proteome of Raji cells treated with ADR were analyzed by twodimensional differential in-gel electrophoresis (2D-DIGE) coupled with linear ion trap quadrupole-electrospray ionization tandem mass spectrometry (LTQ-ESI-MS/MS). The altered patterns of three identified proteins were validated by Western blot and analyzed by pathway studio software. The results showed that 34 proteins were downregulated and 3 proteins upregulated in the study group compared with the control group. The differentially expressed proteins distributed their functions in reduction-oxidation reactions, DNA repair, cell cycle regulation, transporters and channels, and oxidative phosphorylation. Furthermore, heat shock protein 70 (HSP70), ATP-binding cassette transporter isoform B6 (ABCB6), and prohibitin (PHB) identified in this study may be closely related to chemoresistance and could serve as potential chemotherapeutic targets for NHL. Collectively, these results suggest that specific mitochondrial proteins are uniquely susceptible to alterations in abundance following exposure to ADR and carry implications for the investigation of therapeutic and prognostic markers. Further studies focusing on these identified proteins will be used to predict treatment response and reverse apoptosis resistance, and to explore drug-combination strategies associated with ADR for NHL therapy.

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Section: Mtt Analysismentioning

confidence: 99%

Comparative Mitochondrial Proteomic Analysis of Raji Cells Exposed to Adriamycin

Jiang

Sun

Fang

et al. 2009

Mol Med

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“…Litvinova et al [43] tested the effects of G-CSF combined with cytosine-arabinoside, etoposide or doxoru- Fig. 4 Drug sensitivity of KG-1 cells to daunorubicin after pretreatment with different concentrations of G-CSF.…”

Section: Discussionmentioning

confidence: 99%

Granulocyte Colony Stimulating Factor Increases Drug Resistance of Leukaemic Blast Cells to Daunorubicin

Márkász

Hajas

Kiss

et al. 2008

Pathol. Oncol. Res.

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Acute leukaemia is known as the most common cancer in childhood. Febrile neutropenia is a common serious side effect of the cytostatic treatment of malignancies. The clinical use of Granulocyte Colony Stimulating Factor (G-CSF) has become widespread to minimize chemotherapy-induced myelosuppression and febrile neutropenia in childhood solid tumors, acute lymphoid leukaemia (ALL) and in several trials with AML. In case of ALL this seems to be reasonable because, due to the absence of G-CSF receptor (G-CSFR) on the surface of normal lymphoid cells, G-CSF does not have any influence on the pathways of proliferation and differentiation of lymphoid lineage cells. It has been suggested, however, that ALL blasts with B or T cell surface antigens as well as biphenotypic leukaemia cells express G-CSFR, and they are able to respond to exogenously added G-CSF with proliferation. In this study we investigated how G-CSF might influence the sensitivity of leukemic cells to daunorubicin induced cell death using MTT assay, flow cytometry and Western blot analysis. After pretreatment of KG-1 leukaemic cells with G-CSF a moderate increase in the resistance of these cells to daunorubicin could be observed. These results draw attention to the risk of G-CSF application as an adjuvant therapy of childhood ALL. In addition, adjuvant treatment of AML patients with G-CSF in order to prevent neutropenia, or its use in priming regimens might result resistance to daunorubicin.

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“…Single doses of 2, 4, and 6 Gy were given and the cells were subsequently cultured for various times (24,48, and 72 h) for analysis. Cell proliferation was evaluated by the MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide] assay as described previously [10]. As shown in Fig.…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of Mitochondria in Raji Cells Following Exposure to Radiation: Implications for Radiotherapy Response

Jiang

Liu²,

Fang³

et al. 2009

PPL

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Radioresistance represents a major obstacle to a successful outcome for the treatment of non-Hodgkin's lymphoma (NHL). Here we performed a global differential proteome analysis of the mitochondria in Raji cells exposed to radiation. The results showed that 23 differentially expressed proteins were identified. Furthermore, GAPDH, RECQL4, MKI67, and ATAD3B could serve as potential biomarkers of radioresistance.

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Effects of CSFS and their Combinations with Chemotherapeutic Agents (CH) on Leukemic Blasts (LB) in Children (MTT-Assay)

Cited by 3 publications

References 12 publications

Comparative Mitochondrial Proteomic Analysis of Raji Cells Exposed to Adriamycin

Comparative Mitochondrial Proteomic Analysis of Raji Cells Exposed to Adriamycin

Granulocyte Colony Stimulating Factor Increases Drug Resistance of Leukaemic Blast Cells to Daunorubicin

Proteomic Analysis of Mitochondria in Raji Cells Following Exposure to Radiation: Implications for Radiotherapy Response

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