Effects of cyclic AMP and protein kinase on calcium uptake in a microsomal fraction from guinea pig taenia caecum

Hisayama, Tetsuhiro; Takayanagi, Issei

doi:10.1016/0006-2952(83)90204-6

Cited by 21 publications

(4 citation statements)

References 27 publications

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“…It is, therefore, likely that the relaxants exert their effect on the plasma membrane to produce relaxation, although, in addition to this, intracellular actions may also be involved. The relaxant effect of,-receptor agonists is generally believed to be due to intracellular Ca sequestration and/or Ca extrusion from the cell, effected by an increase in cyclic AMP (Hardman, 1981;Hisayama & Takayanagi, 1983). The fact that forskolin which activates adenylate cyclase and theophylline which inhibits phosphodiesterase produce relaxation also suggests an important role ofcyclic AMP in relaxation (Seamon & Daly, 1981;Muller & Baer, 1983), although the degree of relaxation is not quantitatively correlated with an increase in cyclic AMP concentrations (Fredholm et al, 1979;Kolbeck et al, 1979;Vegesna & Diamond, 1983;1984).…”

Section: Discussionmentioning

confidence: 99%

Effects of relaxants on electrical and mechanical activities in the guinea‐pig tracheal muscle

Honda

Satake

Takagi

et al. 1986

British J Pharmacology

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1 In isolated tracheal musces of the guinea-pig, effects of several relaxants were studied by simultaneously recording the membrane potential and mechanical response. 2 Intracellular recordings showed regular slow waves in most preparations. There was a close correlation between membrane potential and slow wave amplitude. The linear regression line of the slow wave amplitude (Y mV) on the membrane potential (X mV) could be expressed by Y = -0.35X -5.9. The mean values of resting potential and slow wave amplitude were -50.6 ± 0.6 m.V a-nd 11.9 ± 0.5 mV, respectively. 3 Relaxant drugs used (isoprenaline, terbutaline, adrenaline, noradrenaline, theophylline, forskolin and dibutyryl cyclic AMP) all produced hyperpolarization of the membrane and abolished the slow wave. The degree of relaxation was closely related to these electrical responses, although the recovery of electrical responses was faster than the mechanical response. 4 It was concluded that the relaxation caused by the agents, which are known to increase the intracellular cyclic AMP level, was accompanied by a clear hyperpolarization and suppression of slow waves.

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Section: Discussionmentioning

confidence: 99%

Effects of relaxants on electrical and mechanical activities in the guinea‐pig tracheal muscle

Honda

Satake

Takagi

et al. 1986

British J Pharmacology

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“…The supernatant was again cen trifuged at 15,000 x g for 20 min. Centrifugation of this supernatant at 100,000 x g for 60 min resulted in a pellet that was used as the microsomal fraction (23).…”

Section: Preparation Of Microsomal Fractionsmentioning

confidence: 99%

Involvement of β3-Adrenoceptor in the Relaxation Response in Guinea Pig Taenia Caecum

Koike

Takayanagi

Muramatsu

et al. 1994

Japanese Journal of Pharmacology

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ABSTRACT-13-Adrenoceptors in the guinea pig taenia caecum were investigated by measuring relaxation responses to agonists and by a radioligand binding assay using [3H]CGP 12177. The rightward shift of the isoprenaline concentration-response curve was observed by butoxamine, a p2-selective antagonist, and the pA2 value for butoxamine was 6.46. In control preparations, catecholamines caused relaxation with the following rank order of potency: isoprenaline > adrenaline > noradrenaline. However, in the presence of 10-6 M phentolamine, 3 x 10-4 M atenolol and 10-4 M butoxamine, the rank order of potency of the agonists was: isoprenaline > noradrenaline > adrenaline. CGP 12177 caused graded relaxation of the guinea pig taenia caecum, and this response was not influenced by 10-6 M phentolamine, 3 X 10-4 M atenolol, 10-4 M butoxamine or 10-6 M propranolol. The Scatchard plot of the specific [3H]CGP 12177 binding to microsomal fractions from the guinea pig taenia caecum showed two affinity sites of the receptor: high affin ity (KD=0.64 nM) and low affinity (KD=142.21 nM) sites. The pKD value of the high affinity site of [3H] CGP 12177 was in agreement with its pA2 value, and that of the low affinity site was in agreement with its pD2 value. These results suggest that isoprenaline-, noradrenaline and adrenaline-induced relaxations of the guinea pig taenia caecum predominantly involve /2 and (33-adrenoceptors, whereas CGP 12177-induced relaxation is mediated solely through 33-adrenoceptors.

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“…The relaxing effect of f-adrenoceptor agonists involves adenosine 3': 5'-cyclic monophosphate (cyclic AMP) (Takayanagi et al, 1972;Kramer & Hardman, 1980), and cyclic AMP stimulates 45Ca2+ uptake by smooth muscle microsomes (Fitzpatrick & Szentivanyi, 1977, Hisayama & Takayanagi, 1983Raeymaekers et al, 1988). However, few studies have critically evaluated the cause-effect relationship between the increased Ca2+ uptake by the stores and relaxation in intact smooth muscle preparations (Morgan & Morgan, 1984).…”

Section: Introductionmentioning

confidence: 99%

Ryanodine reveals multiple contractile and relaxant mechanisms in vascular smooth muscle: simultaneous measurements of mechanical activity and of cytoplasmic free Ca²⁺ level with fura‐2

Hisayama

Takayanagi

Okamoto

1990

British J Pharmacology

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1 The effects of ryanodine on changes in cytoplasmic Ca2+ level ([Ca2+]i) and muscle tension induced by maximum concentrations of phenylephrine (Phe; 1 pM), prostaglandin F2, (PGF2,, 10UM), caffeine (Caf, 30 mM) and isoprenaline (Iso, 1 M) were examined in rat aortic strips using fura-2. Treatment of the preparation with ryanodine had no effect on the concentration-response curves for Iso in relaxing the 0.1 M Phe-or 40 mm K+-induced precontraction.7 It is suggested that Phe and Caf mobilize Ca2 + from a ryanodine-sensitive Ca2 + store and that PGF2.releases Ca2+ from a ryanodine-insensitive Ca2+ store. The former contributes to the transient contraction through a Ca2'-dependent process, while the latter seems not to be directly associated with the contraction. The sustained contraction under Ca2 +-free conditions might involve a Ca2 '-independent process or a change in the sensitivity of the contractile filaments to Ca2 + 8 In addition to lowering cytoplasmic Ca2+ concentration, it is suggested that Iso counteracts the apparently Ca2 +-independent process. The ryanodine-sensitive Ca2+ store plays no substantial role in active relaxation by Iso, although it does play a major role in the maintenance of cytoplasmic Ca2+ in a quiescent muscle.

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Effects of cyclic AMP and protein kinase on calcium uptake in a microsomal fraction from guinea pig taenia caecum

Cited by 21 publications

References 27 publications

Effects of relaxants on electrical and mechanical activities in the guinea‐pig tracheal muscle

Effects of relaxants on electrical and mechanical activities in the guinea‐pig tracheal muscle

Involvement of β3-Adrenoceptor in the Relaxation Response in Guinea Pig Taenia Caecum

Ryanodine reveals multiple contractile and relaxant mechanisms in vascular smooth muscle: simultaneous measurements of mechanical activity and of cytoplasmic free Ca²⁺ level with fura‐2

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Effects of cyclic AMP and protein kinase on calcium uptake in a microsomal fraction from guinea pig taenia caecum

Cited by 21 publications

References 27 publications

Effects of relaxants on electrical and mechanical activities in the guinea‐pig tracheal muscle

Effects of relaxants on electrical and mechanical activities in the guinea‐pig tracheal muscle

Involvement of β3-Adrenoceptor in the Relaxation Response in Guinea Pig Taenia Caecum

Ryanodine reveals multiple contractile and relaxant mechanisms in vascular smooth muscle: simultaneous measurements of mechanical activity and of cytoplasmic free Ca2+ level with fura‐2

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Ryanodine reveals multiple contractile and relaxant mechanisms in vascular smooth muscle: simultaneous measurements of mechanical activity and of cytoplasmic free Ca²⁺ level with fura‐2