Effects of cysteine and ergothioneine on post-thawed Merino ram sperm and biochemical parameters

Çoyan, Kenan; Başpınar, Nuri; Bucak, Mustafa Numan; Akalın, Pınar Peker

doi:10.1016/j.cryobiol.2011.04.001

Cited by 65 publications

(41 citation statements)

References 54 publications

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“…It was observed that the percentage of motility in LE group was significantly higher cystein group and the more addition dose was increased the success of study increased in LE group. The motility percentages were significantly lower than those of the results in the study of Çoyan et al [34] similarly, in the present study, LE group was higher than cystein group. Moreover, Ari et al [35] determined that 10 mM LE improved motility and membrane integrity in replication with poor freezability compared with 0, 1, 2, 5 mM LE.…”

Section: Discussioncontrasting

confidence: 56%

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Farklı Kriyoprotektanların Eritme Sonrası Arı Sperm Kalitesi Üzerine Etkileri

Alçay¹,

Üstüner²,

Çakmak³

et al. 2015

Kafkas Univ Vet Fak Derg

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The aim of this study was to determine the influence of different doses of L-Ergothioneine (LE), cystein and N-acetylcystein (NAC) on post-thawing semen parameters in rams. Ejaculates collected from four Tushin Rams were evaluated and pooled at 35°C. Semen samples were diluted with skim-milk based extender containing LE (5, 10 mM), Cystein (5, 10 mM), NAC (5, 10 mM) or not containing any antioxidant (control) and loaded to 0.25 ml French straws. Straws were cooled to 5°C for 2 h, frozen in liquid nitrogen vapour (aprox. -120°C) for 15 min and then being stored in liquid nitrogen until thawing process. Straws were thawed in water bath (37°C for 1 min). The percentages of motility, viability, abnormal acrosome, total abnormalities, membrane integrity (hypoosmatic swelling test, HOST) were statistically assessed. Also total antioxidant capacity (TAC) and total oxidatif stres (TOS) were evaluated in samples from replications. It was seen that LE was superior to N-acetylcystein and cystein in motility, viability, defected acrosome, total morphological abnormalities, HOST and TAC, (P<0.05) except cystein in motility. Neverthless, there was not any statistically difference between LE and control groups (P>0.05). It was conlcluded that there was not any beneficial or detrimental effects of LE on post thawing semen parameters in rams while it was determined that cystein and NAC may have been some detrimental effects on post thawing semen parameters in rams. These results warrants future scientific studies on LE, NAC and cystein in ram semen cryopreservation. Keywords: Ram semen, Cryopreservation, L-Ergothioneine, Cystein, N-acetylcystein, Tushin Koç Spermasının Dondurulması Üzerine L-Ergothionin, N-asetil sistein ve Sisteinin Etkileri ÖzetBu çalışmanın amacı, koçlarda L-Ergothionin (LE), sistein ve N-asetil sisteinin (NAC) farklı dozlarının çözüm sonu sperma parametreleri üzerine etkisini belirlemekti. Dört Tuj ırkı koçtan alınan ejakülatlar değerlendirildi ve 35°C'de karışım yapıldı. Sperma örnekleri, LE (5, 10 mM), Sistein (5, 10 mM), NAC (5, 10 mM) içeren veya antioksidan içermeyen (kontrol), yağsız süt temelli sulandırıcıyla sulandırılarak, 0.25 ml'lik payetlere dolduruldu. Payetler 5°C'ye 2 saat süreyle soğutuldu, sıvı azot buharında (yaklaşık -120°C) 15 dak. süre ile donduruldu ve çözme işlemine kadar sıvı azot içinde saklandı. Payetler su banyosunda (37°C'de 1 dak.) çözüldü. Motilite, canlılık, anormal akrozom, toplam anormalite ve membran bütünlüğü (hipoozmotik şişme testi, HOST) yüzdeleri istatistiksel olarak değerlendirildi. Ayrıca, toplam antioksidan kapasite (TAK) ve toplam oksidatif stres (TOS), replikasyonlardan elde edilen örneklerde değerlendirildi. LE'nin motilite (sistein hariç), canlılık, akrozom hasarı, total morfolojik anormalite, HOST ve TAK oranları bakımından, NAC ve sisteine üstünlük sağladığı görüldü (P<0.05). Yine de, LE ve kontrol grubu arasında istatistiksel bir fark yoktu (P>0.05). Sistein ve NAC'ın koçlarda çözüm sonu spermatolojik parametreler üzerine bazı olumsuz etkileri belirlen...

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Section: Discussioncontrasting

confidence: 56%

“…Compared with Çoyan et al [34] study in ram semen, the findings obtained from control and LE groups of the present study were higher and similar to those of N-acetylcysteine and cysteine added.…”

Section: Discussioncontrasting

confidence: 43%

Farklı Kriyoprotektanların Eritme Sonrası Arı Sperm Kalitesi Üzerine Etkileri

Alçay¹,

Üstüner²,

Çakmak³

et al. 2015

Kafkas Univ Vet Fak Derg

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“…In addition, it is an intracellular glutathione precursor. When sperm is cooled, cysteine prevents the generation of ROS and protect the spermatozoa from LPO (Coyan et al 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Each pooled ejaculate was split into five equal aliquots and diluted at 37 °C with base extenders containing trehalose (10 and 25 mM), cysteine (2 and 4 mM) or no antioxidant (control), respectively. Specific trehalose and cysteine concentrations were selected based on results obtained from a previous trehalose and cysteine standardisation study with ram and bull semen (Aboagla and Terada 2003;Sariozkan et al 2009;Hu et al 2010;Coyan et al 2011;Jafaroghli et al 2011). The final semen concentration was 4 × 10 8 sperm/ml (single step dilution), in 15-ml polypropylene centrifuge tubes.…”

Section: Methodsmentioning

confidence: 99%

“…Cysteine is a lowmolecular weight amino acid containing a thiol group; it is an intracellular glutathione precursor. In addition, cysteine consists of thiol groups, which individually penetrate into the sperm and act as non-enzymatic antioxidants (Coyan et al 2011;Topraggaleh et al 2014). Cysteine can enhance intracellular glutathione (GSH) biosynthesis, which protects membrane lipids through indirect radical scavenging properties (Bansal and Bilaspuri 2011).…”

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confidence: 99%

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Positive effects of trehalose and cysteine on ram sperm parameters

Güngör¹,

Öztürk²,

Omur³

2017

Vet. Med. - Czech

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ABSTRACT:The aim of this study was to determine the effects of trehalose and cysteine on sperm motility, viability, mitochondrial activity and acrosome integrity during liquid storage of Merino ram semen. Ejaculates were collected using artificial vaginas from five Merino rams, microscopically evaluated and pooled at 37 °C. The pooled semen samples were diluted in a Tris-based extender, including cysteine (2 mM and 4 mM), trehalose (10 mM and 25 mM) and no antioxidant (control). Diluted semen samples were kept in tubes and cooled from 37 to 5 °C in a cold cabinet, and maintained at 5 °C. Cooled samples were evaluated for sperm motility, viability, mitochondrial activity and acrosome integrity at 0, 24, 48, 72 and 96 h. Extender supplemented with trehalose (10 and 25 mM) and cysteine (2 and 4 mM) led to higher motility in comparison to the control at 24, 48, 72 and 96 h of liquid storage (P < 0.05). Trehalose at the doses of 10 mM, 25 mM and 2 mM cysteine led to higher viability between 24-48-72 h and at 96 h of liquid storage (P < 0.05). Further, 4 mM of cysteine improved sperm viability rates at 24 and 48 h of storage compared to the control group (P < 0.05), and resulted in improved acrosome integrity rates compared to the control group at 72 and 96 h of storage (P < 0.05). Extender supplemented with 10 and 25 mM trehalose at 24 and 72 h and 4 mM cysteine at 24 and 96 h of storage led to higher sperm mitochondrial activity rates when compared to the control group (P < 0.05). In conclusion, the findings of this study show that trehalose and cysteine provided significant protection to ram sperm parameters during liquid storage.

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