Farklı Kriyoprotektanların Eritme Sonrası Arı Sperm Kalitesi Üzerine Etkileri

Abstract

The aim of this study was to determine the influence of different doses of L-Ergothioneine (LE), cystein and N-acetylcystein (NAC) on post-thawing semen parameters in rams. Ejaculates collected from four Tushin Rams were evaluated and pooled at 35°C. Semen samples were diluted with skim-milk based extender containing LE (5, 10 mM), Cystein (5, 10 mM), NAC (5, 10 mM) or not containing any antioxidant (control) and loaded to 0.25 ml French straws. Straws were cooled to 5°C for 2 h, frozen in liquid nitrogen vapour (aprox. -120°C) for 15 min and then being stored in liquid nitrogen until thawing process. Straws were thawed in water bath (37°C for 1 min). The percentages of motility, viability, abnormal acrosome, total abnormalities, membrane integrity (hypoosmatic swelling test, HOST) were statistically assessed. Also total antioxidant capacity (TAC) and total oxidatif stres (TOS) were evaluated in samples from replications. It was seen that LE was superior to N-acetylcystein and cystein in motility, viability, defected acrosome, total morphological abnormalities, HOST and TAC, (P<0.05) except cystein in motility. Neverthless, there was not any statistically difference between LE and control groups (P>0.05). It was conlcluded that there was not any beneficial or detrimental effects of LE on post thawing semen parameters in rams while it was determined that cystein and NAC may have been some detrimental effects on post thawing semen parameters in rams. These results warrants future scientific studies on LE, NAC and cystein in ram semen cryopreservation. Keywords: Ram semen, Cryopreservation, L-Ergothioneine, Cystein, N-acetylcystein, Tushin Koç Spermasının Dondurulması Üzerine L-Ergothionin, N-asetil sistein ve Sisteinin Etkileri ÖzetBu çalışmanın amacı, koçlarda L-Ergothionin (LE), sistein ve N-asetil sisteinin (NAC) farklı dozlarının çözüm sonu sperma parametreleri üzerine etkisini belirlemekti. Dört Tuj ırkı koçtan alınan ejakülatlar değerlendirildi ve 35°C'de karışım yapıldı. Sperma örnekleri, LE (5, 10 mM), Sistein (5, 10 mM), NAC (5, 10 mM) içeren veya antioksidan içermeyen (kontrol), yağsız süt temelli sulandırıcıyla sulandırılarak, 0.25 ml'lik payetlere dolduruldu. Payetler 5°C'ye 2 saat süreyle soğutuldu, sıvı azot buharında (yaklaşık -120°C) 15 dak. süre ile donduruldu ve çözme işlemine kadar sıvı azot içinde saklandı. Payetler su banyosunda (37°C'de 1 dak.) çözüldü. Motilite, canlılık, anormal akrozom, toplam anormalite ve membran bütünlüğü (hipoozmotik şişme testi, HOST) yüzdeleri istatistiksel olarak değerlendirildi. Ayrıca, toplam antioksidan kapasite (TAK) ve toplam oksidatif stres (TOS), replikasyonlardan elde edilen örneklerde değerlendirildi. LE'nin motilite (sistein hariç), canlılık, akrozom hasarı, total morfolojik anormalite, HOST ve TAK oranları bakımından, NAC ve sisteine üstünlük sağladığı görüldü (P<0.05). Yine de, LE ve kontrol grubu arasında istatistiksel bir fark yoktu (P>0.05). Sistein ve NAC'ın koçlarda çözüm sonu spermatolojik parametreler üzerine bazı olumsuz etkileri belirlen...

Arı Spermasının Protein Eklenmiş TL-Hepes Bazlı Sulandırıcı İle Dondurulması

“…In our study, the HOST rates in BSA3 and BSA5 group were higher than in the other groups (P<0.05). The HOST values have common points for the results with the previous research [12] .…”

“…In the study, BSA5 group yielded higher motility rates than other groups at post thaw time point (P<0.05). The post-thaw motility values of drone semen cryopreserved with different extenders ranged between 25%-62% [8,11,12,24] . After thawing, the motility rate in BSA5 group has a common point with the findings of other studies.…”

“…Sexually mature drones (15 days or more) were used for sperm collection. Ejaculation was triggered by exerting pressure on the thorax and then gently squeezing the abdomen [12] . Drone semen was taken up into the Schley syringe under a stereo microscope.…”

“…The same person conducted the processes and measurements along the research. Semen motility was determined using a phase-contrast microscope (Nikon Alphaphot YS, Japan) (400×) with a warm slide that heated to 37°C and the motility results were expressed in percent [12] .…”

“…Drone semen cryopreservation has been accomplished in recent years. However, the freezing success of drone semen has not reached the desired level nowadays [11,12] .…”